为获得耐高浓度丙烯腈的菌株,利用紫外线对珊瑚诺卡氏菌进行诱变,并将诱变后生长情况较好的突变株在含一定浓度丙烯腈的培养基中进行筛选.结果表明:在紫外灯功率为20 W,照射距离为10 cm的条件下,诱变时间为3 m in和4 m in,致死率均大于9...为获得耐高浓度丙烯腈的菌株,利用紫外线对珊瑚诺卡氏菌进行诱变,并将诱变后生长情况较好的突变株在含一定浓度丙烯腈的培养基中进行筛选.结果表明:在紫外灯功率为20 W,照射距离为10 cm的条件下,诱变时间为3 m in和4 m in,致死率均大于90%;而诱变时间为5 m in,致死率为100%.诱变之后,从含低浓度丙烯腈的的培养基中,筛选出了9株有利突变的菌株.通过在含高浓度丙烯腈的培养基中复筛得到2株耐5.82%丙烯腈的突变株,其诱变时间为4 m in.展开更多
A SPE-HPLC method has been developed for the determination of pesticide malathion residue in garden spgarden stuff.The research method showed that the liner relationship between peak area and sample size was satisfact...A SPE-HPLC method has been developed for the determination of pesticide malathion residue in garden spgarden stuff.The research method showed that the liner relationship between peak area and sample size was satisfactory in the range of 0.5 μg·mL-1～50.0μg·mL-1,the coefficient of correlation(r2) was 0.9992;the limit of detection(LOD)was 0.05 μg·kg-1 and the limit of quantification(LOQ) was 0.5μg·mL-1;the recoveries were 86.3%～98.0% at the spiked levels of 0.5,30.0 and 50.0 μg·mL-1with the relative standard deviation(RSD) of 9.1%～0.6%.展开更多
In this paper,one novel Schiff base(HL) derived from 1-phenyl-3-methyl-4-benzoyl-pyrazolone-5(PMBP) and sulfanilamide and its corresponding transitional metallic copper complex have been synthesized in the nonaqueous ...In this paper,one novel Schiff base(HL) derived from 1-phenyl-3-methyl-4-benzoyl-pyrazolone-5(PMBP) and sulfanilamide and its corresponding transitional metallic copper complex have been synthesized in the nonaqueous solvent.The formula of complex is CuL2 based on elemental analysis,complexometric titration,molar conductance value and mass spectra data.Its molecular structure is characterized by means of IR,TG and 1HNMR,etc.,and the antibacterial activities under different concentrations of these two compounds are also measured with Oxford cup method.展开更多
文摘为获得耐高浓度丙烯腈的菌株,利用紫外线对珊瑚诺卡氏菌进行诱变,并将诱变后生长情况较好的突变株在含一定浓度丙烯腈的培养基中进行筛选.结果表明:在紫外灯功率为20 W,照射距离为10 cm的条件下,诱变时间为3 m in和4 m in,致死率均大于90%;而诱变时间为5 m in,致死率为100%.诱变之后,从含低浓度丙烯腈的的培养基中,筛选出了9株有利突变的菌株.通过在含高浓度丙烯腈的培养基中复筛得到2株耐5.82%丙烯腈的突变株,其诱变时间为4 m in.
文摘A SPE-HPLC method has been developed for the determination of pesticide malathion residue in garden spgarden stuff.The research method showed that the liner relationship between peak area and sample size was satisfactory in the range of 0.5 μg·mL-1～50.0μg·mL-1,the coefficient of correlation(r2) was 0.9992;the limit of detection(LOD)was 0.05 μg·kg-1 and the limit of quantification(LOQ) was 0.5μg·mL-1;the recoveries were 86.3%～98.0% at the spiked levels of 0.5,30.0 and 50.0 μg·mL-1with the relative standard deviation(RSD) of 9.1%～0.6%.
文摘In this paper,one novel Schiff base(HL) derived from 1-phenyl-3-methyl-4-benzoyl-pyrazolone-5(PMBP) and sulfanilamide and its corresponding transitional metallic copper complex have been synthesized in the nonaqueous solvent.The formula of complex is CuL2 based on elemental analysis,complexometric titration,molar conductance value and mass spectra data.Its molecular structure is characterized by means of IR,TG and 1HNMR,etc.,and the antibacterial activities under different concentrations of these two compounds are also measured with Oxford cup method.
