通过富集分离筛选高效长链烷烃降解菌,采用形态学、生理生化实验、16S r DNA序列比对进行菌种鉴定,通过UV和GC-MS检测菌株对长链烷烃降解特性。结果表明:分离到1株长链烷降解烃菌,经鉴定为希瓦氏菌属,命名为Shewanella sp.LZ02。该菌生...通过富集分离筛选高效长链烷烃降解菌,采用形态学、生理生化实验、16S r DNA序列比对进行菌种鉴定,通过UV和GC-MS检测菌株对长链烷烃降解特性。结果表明:分离到1株长链烷降解烃菌,经鉴定为希瓦氏菌属,命名为Shewanella sp.LZ02。该菌生长温度范围0~45℃,最适生长温度35℃,在接种量为106CFU/m L,原油浓度为0.5%(W/V),振荡培养7 d时,降解率可达62.90%。GC-MS分析表明:石油中长链烷烃C15~C21被完全降解,C22~C32部分降解,降解方式为单末端氧化。展开更多
基金supported by the National Natural Science Foundation of China(51203138,51273179)Intenatonal S&T Cooperation Program,China(2012DFA51210)+1 种基金Natural Science Foundation of Zhejiang Province,China(LY15E030006)Natural Science Foundation of Zhejiang University of Technology,China(1401101002408)~~
基金supported by the National Natural Science Foundation of China(Nos.21404124,51572303)the Program of Innovative Talent(in Science and Technology)in University of Henan Province,China(No.17HASTIT007)+3 种基金the Project of Innovation Scientists and Technicians Troop Construction Projects of Henan Province,China(No.2013259)the Project of Henan Province Key Discipline of Applied Chemistry,China(No.201218692)the Program for Innovative Research Team(in Science and Technology)in University of Henan Province,China(No.14IRTSTHN009)the Project of Science and Technology Program of Henan Province,China(No.162102310591)~~
