Glutaraldehyde crosslinked chitosan used in the adsorption upon model uremic middle molecular toxins was studied. In comparison with untreated glutaraldehyde crosslinked chitosan resin beads, the hydrogen reduced ones...Glutaraldehyde crosslinked chitosan used in the adsorption upon model uremic middle molecular toxins was studied. In comparison with untreated glutaraldehyde crosslinked chitosan resin beads, the hydrogen reduced ones showed a quite realistic behavior in the adsorption upon those model toxins, and the amount of adsorption was fairly high while the equilibrium time was obviously shortened.展开更多
Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peak...Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic serum and urine were less than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 8 to 9 subpeaks at 230 nm by anion exchange chromatography. One of these subpeaks, A 3, was detected in uremic serum and normal urine but undetectable in uremic urine. After a gel permeation chromatography with bidistilled water as eluent for desalting, subfraction A 3 was seperated into two parts designated A 3 Ⅰ and A 3 Ⅱ in order. The results of MALDI TOF MS revealed that the two peaks from both samples were identical respectively, fraction A 3 Ⅰ contained three kinds of components with molecular weight 839.69, 1 007.94 and 2 015.16 and fraction A 3 Ⅱ consisted of other three kinds of components with molecular weight 873.69, 1 106.67 and 1 680.28.展开更多
Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular pe...Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular peaks（A and B） were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight {1 214}, which could hardly be seen in the fraction A-Ⅰ of uremic urine.展开更多
In this paper, we use glucose as the contrast to study the mechanism of β-Dextrine and fixed β-Dextrine acting on the bilirublin. The results show that the hydrogen bond is the main force between β-cyclodextrin and...In this paper, we use glucose as the contrast to study the mechanism of β-Dextrine and fixed β-Dextrine acting on the bilirublin. The results show that the hydrogen bond is the main force between β-cyclodextrin and bilirubin. After the adsorption of bilirubin for 2 h, the adsorption capacity is over 90 %.展开更多
The endotoxin adsorbents were prepared by coupling divinyl amine ligands on crosslinked chitosan matrix. Their adsorption ability to endotoxin was compared. It was found that the adsorbent with polyethylene polyamine ...The endotoxin adsorbents were prepared by coupling divinyl amine ligands on crosslinked chitosan matrix. Their adsorption ability to endotoxin was compared. It was found that the adsorbent with polyethylene polyamine ligand had the highest adsorption capacity and its clearance percent for endotoxin in endotoxemia plasma was over 80%. The results also showed that the adsorbent has good blood compatibility and clinical application foreground.展开更多
本文报道用a-萘胺和琥珀酸酐合成1-萘基-琥珀酰亚胺(NPr),并用FT-IR、DSC、X-射线衍射和荧光等方法研究了该化合物的结构与性能.DSC和X-射线衍射结果表明,纯净NPr的熔点与John L Hubbard报道结果(147～149℃)不同,应为(158.3±0.2...本文报道用a-萘胺和琥珀酸酐合成1-萘基-琥珀酰亚胺(NPr),并用FT-IR、DSC、X-射线衍射和荧光等方法研究了该化合物的结构与性能.DSC和X-射线衍射结果表明,纯净NPr的熔点与John L Hubbard报道结果(147～149℃)不同,应为(158.3±0.2℃);熔融淬火样品仍存在部分结晶,并且在一定温度下发生冷结晶;FT-IR结果表明羰基伸缩振动产生反对称振动(1705cm-1)和对称振动(1779cm-1)的能级分裂;荧光光谱结果则进一步表明该化合物的电子云排布结构与单体萘环相似,其原因可归于羰基的诱导效应和共轭效应对氮原子的影响.展开更多
Biodegradable triblock copolymer PLA/PEG/PLA was synthesized by ring-opening bulk polymerization of D,L-lactide in the presence of poly(ethylene glycol) (PEG), in the molecular structure of which, the length of PEG an...Biodegradable triblock copolymer PLA/PEG/PLA was synthesized by ring-opening bulk polymerization of D,L-lactide in the presence of poly(ethylene glycol) (PEG), in the molecular structure of which, the length of PEG and PLA chain segments was made to be quite different. Nanoparticles were prepared by using the copolymer via a double emulsion-evaporation technique. The paticles tended to form the configuration like capsules, i.e., the nanocapsules, because of the great size difference in PEG and PLA segments of the copolymer. Insulin, chosen as a model drug, was encapsulated into nanocapsules. The effect of preparation conditions on the size, insulin encapsulation efficiency, and in vitro drug release behavour of the nanoparticles were investigated. The experimental results show that the nanocapsules had a smooth spherical surface and the mean diameter was in the range from 180 nm to 350 nm, and the entrapment of insulin achieved up to 78.4. The drug-loaded nanocapsules released their content continuously, remarkably different from the corresponding micelles which gave a significant initial burst release followed by a slow release.展开更多
Thermal spectra of liquid crystal, \%p\|n\%\|dodecyloxy benzoic acid were collected at 26\150 ℃ by FTIR microspectroscopy. Conformational apparent enthalpy differences Δ\%H\% in phase transitions have been determine...Thermal spectra of liquid crystal, \%p\|n\%\|dodecyloxy benzoic acid were collected at 26\150 ℃ by FTIR microspectroscopy. Conformational apparent enthalpy differences Δ\%H\% in phase transitions have been determined by vant Hoff equation at constant pressure and plotted with temperature. Compared to DSC, these enthalpies plot with temperature could be divided into three types. The first one was long alkyl chain group which has only one apparent endothermic enthalpy peak at 95 ℃. The second one was partial carbonyl and aromatic ether groups has three endothermic enthalpy peaks in the phase transition. The third type including benzoyl group which has one exothermic enthalpy peak at 95 ℃ and a broad endothermic enthalpy peak in the range of 128\140 ℃. These differences indicated that oxy\|benzoic acid part of dodecyloxy benzoic acid recrystallized to cycle dimer during alkyl chain melting in crystal\|smectic phase transition.展开更多
文摘Glutaraldehyde crosslinked chitosan used in the adsorption upon model uremic middle molecular toxins was studied. In comparison with untreated glutaraldehyde crosslinked chitosan resin beads, the hydrogen reduced ones showed a quite realistic behavior in the adsorption upon those model toxins, and the amount of adsorption was fairly high while the equilibrium time was obviously shortened.
文摘Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic serum and urine were less than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 8 to 9 subpeaks at 230 nm by anion exchange chromatography. One of these subpeaks, A 3, was detected in uremic serum and normal urine but undetectable in uremic urine. After a gel permeation chromatography with bidistilled water as eluent for desalting, subfraction A 3 was seperated into two parts designated A 3 Ⅰ and A 3 Ⅱ in order. The results of MALDI TOF MS revealed that the two peaks from both samples were identical respectively, fraction A 3 Ⅰ contained three kinds of components with molecular weight 839.69, 1 007.94 and 2 015.16 and fraction A 3 Ⅱ consisted of other three kinds of components with molecular weight 873.69, 1 106.67 and 1 680.28.
文摘Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular peaks（A and B） were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight {1 214}, which could hardly be seen in the fraction A-Ⅰ of uremic urine.
文摘In this paper, we use glucose as the contrast to study the mechanism of β-Dextrine and fixed β-Dextrine acting on the bilirublin. The results show that the hydrogen bond is the main force between β-cyclodextrin and bilirubin. After the adsorption of bilirubin for 2 h, the adsorption capacity is over 90 %.
文摘The endotoxin adsorbents were prepared by coupling divinyl amine ligands on crosslinked chitosan matrix. Their adsorption ability to endotoxin was compared. It was found that the adsorbent with polyethylene polyamine ligand had the highest adsorption capacity and its clearance percent for endotoxin in endotoxemia plasma was over 80%. The results also showed that the adsorbent has good blood compatibility and clinical application foreground.
文摘本文报道用a-萘胺和琥珀酸酐合成1-萘基-琥珀酰亚胺(NPr),并用FT-IR、DSC、X-射线衍射和荧光等方法研究了该化合物的结构与性能.DSC和X-射线衍射结果表明,纯净NPr的熔点与John L Hubbard报道结果(147～149℃)不同,应为(158.3±0.2℃);熔融淬火样品仍存在部分结晶,并且在一定温度下发生冷结晶;FT-IR结果表明羰基伸缩振动产生反对称振动(1705cm-1)和对称振动(1779cm-1)的能级分裂;荧光光谱结果则进一步表明该化合物的电子云排布结构与单体萘环相似,其原因可归于羰基的诱导效应和共轭效应对氮原子的影响.
文摘Biodegradable triblock copolymer PLA/PEG/PLA was synthesized by ring-opening bulk polymerization of D,L-lactide in the presence of poly(ethylene glycol) (PEG), in the molecular structure of which, the length of PEG and PLA chain segments was made to be quite different. Nanoparticles were prepared by using the copolymer via a double emulsion-evaporation technique. The paticles tended to form the configuration like capsules, i.e., the nanocapsules, because of the great size difference in PEG and PLA segments of the copolymer. Insulin, chosen as a model drug, was encapsulated into nanocapsules. The effect of preparation conditions on the size, insulin encapsulation efficiency, and in vitro drug release behavour of the nanoparticles were investigated. The experimental results show that the nanocapsules had a smooth spherical surface and the mean diameter was in the range from 180 nm to 350 nm, and the entrapment of insulin achieved up to 78.4. The drug-loaded nanocapsules released their content continuously, remarkably different from the corresponding micelles which gave a significant initial burst release followed by a slow release.
文摘Thermal spectra of liquid crystal, \%p\|n\%\|dodecyloxy benzoic acid were collected at 26\150 ℃ by FTIR microspectroscopy. Conformational apparent enthalpy differences Δ\%H\% in phase transitions have been determined by vant Hoff equation at constant pressure and plotted with temperature. Compared to DSC, these enthalpies plot with temperature could be divided into three types. The first one was long alkyl chain group which has only one apparent endothermic enthalpy peak at 95 ℃. The second one was partial carbonyl and aromatic ether groups has three endothermic enthalpy peaks in the phase transition. The third type including benzoyl group which has one exothermic enthalpy peak at 95 ℃ and a broad endothermic enthalpy peak in the range of 128\140 ℃. These differences indicated that oxy\|benzoic acid part of dodecyloxy benzoic acid recrystallized to cycle dimer during alkyl chain melting in crystal\|smectic phase transition.
