CeTTA4·HP(here,TTA=2-Thenoyltrifluoroacetone,HP=Piperidine)has been synthesized and characterized.we found that the CeTTA4·HP complex can cleave circular plasmid pBR322 DNA at pH7.2 and 37℃.In addition,we c...CeTTA4·HP(here,TTA=2-Thenoyltrifluoroacetone,HP=Piperidine)has been synthesized and characterized.we found that the CeTTA4·HP complex can cleave circular plasmid pBR322 DNA at pH7.2 and 37℃.In addition,we choose BDNPP(bis(2,4-dinitro phenyl)-phosphate)as a model compound to further study the cleavage mechanism for pBR322 DNA.From the second-order kinetics equation,we prove indirectly the mechanism may be hydrolytic cleavage.展开更多
文摘CeTTA4·HP(here,TTA=2-Thenoyltrifluoroacetone,HP=Piperidine)has been synthesized and characterized.we found that the CeTTA4·HP complex can cleave circular plasmid pBR322 DNA at pH7.2 and 37℃.In addition,we choose BDNPP(bis(2,4-dinitro phenyl)-phosphate)as a model compound to further study the cleavage mechanism for pBR322 DNA.From the second-order kinetics equation,we prove indirectly the mechanism may be hydrolytic cleavage.
