作为微纳米科学理论与技术迅猛发展的代表,原子力显微镜(atomic force microscopy,AFM)在其25年的发展过程中极大地推进了生物学在微纳米尺度上的拓展,为微纳米生物学的诞生与发展提供了重要技术手段。本文在介绍AFM基本原理和检测模式...作为微纳米科学理论与技术迅猛发展的代表,原子力显微镜(atomic force microscopy,AFM)在其25年的发展过程中极大地推进了生物学在微纳米尺度上的拓展,为微纳米生物学的诞生与发展提供了重要技术手段。本文在介绍AFM基本原理和检测模式的基础上,结合作者在该领域的研究成果和工作经验,从生物结构与形态学研究、表面物化性质表征、生物大分子的力学操纵三方面综述了AFM在细胞与生物大分子超微结构与生物力学特性研究中的具体应用,并重点探讨了AFM在细胞与生物大分子科学研究中亟待改进和解决的科学与技术问题,提出了一些探讨性的见解和建议。展开更多
We developed a method for patterning organic light emitting molecules with polyaniline(PANI) microstructure as a template.Its microstructure was characterized with atomic force microscopy(AFM).PANI microstructure was ...We developed a method for patterning organic light emitting molecules with polyaniline(PANI) microstructure as a template.Its microstructure was characterized with atomic force microscopy(AFM).PANI microstructure was treated with O2 plasma at low power and short time to get the hydroxyl groups on the silicon substrate,meanwhile,which can minimize the impact on PANI structure.We modified the structure with fluoro-silane reagent through self-assembly to make substrate bearing different surface energies on different areas.Finally,we deposited dyes on substrate by evaporation.The results show that the dyes on the fluoro-silane monolayer can move toward the PANI structure during storage duration and form the structure of organic light emitting molecules.展开更多
文摘作为微纳米科学理论与技术迅猛发展的代表,原子力显微镜(atomic force microscopy,AFM)在其25年的发展过程中极大地推进了生物学在微纳米尺度上的拓展,为微纳米生物学的诞生与发展提供了重要技术手段。本文在介绍AFM基本原理和检测模式的基础上,结合作者在该领域的研究成果和工作经验,从生物结构与形态学研究、表面物化性质表征、生物大分子的力学操纵三方面综述了AFM在细胞与生物大分子超微结构与生物力学特性研究中的具体应用,并重点探讨了AFM在细胞与生物大分子科学研究中亟待改进和解决的科学与技术问题,提出了一些探讨性的见解和建议。
文摘We developed a method for patterning organic light emitting molecules with polyaniline(PANI) microstructure as a template.Its microstructure was characterized with atomic force microscopy(AFM).PANI microstructure was treated with O2 plasma at low power and short time to get the hydroxyl groups on the silicon substrate,meanwhile,which can minimize the impact on PANI structure.We modified the structure with fluoro-silane reagent through self-assembly to make substrate bearing different surface energies on different areas.Finally,we deposited dyes on substrate by evaporation.The results show that the dyes on the fluoro-silane monolayer can move toward the PANI structure during storage duration and form the structure of organic light emitting molecules.
