mtDNAs of T type wheat cytoplasmic male sterile lines Ning Drawf 14(ND14) and their maintainers Ning Drawf 13 (ND13) were isolated and digested completely with restriction endonucleases EcoRI, PstI, EcoRV, BamHI. The ...mtDNAs of T type wheat cytoplasmic male sterile lines Ning Drawf 14(ND14) and their maintainers Ning Drawf 13 (ND13) were isolated and digested completely with restriction endonucleases EcoRI, PstI, EcoRV, BamHI. The results revealed that the molecular structure of mtDNAs from ND14 and ND13 cytoplasms were significantly deviated. The mitochondrial genomic difference between CMS line and maintainers were uncovered by southern hybridization with probes of 18S+5S rRNA、atpA genes from wheat and pea mitochondria, respectively. Due to the complexity of mtDNA and no proof of protein difference, it has not yet been demonstrated whether mtDNA difference of Normal and Male Sterile Cytoplasm of wheat is associated with CMS.展开更多
Rich genetic resources are contained among wheat wild relatives. Cytogenetics Institute of Nanjing Agricultural University commences to transfer these genetic resources from relatives into common wheat. The hybrids be...Rich genetic resources are contained among wheat wild relatives. Cytogenetics Institute of Nanjing Agricultural University commences to transfer these genetic resources from relatives into common wheat. The hybrids between common wheat and Haynaldia villosa, Leymus racemosus,Roegneria ciliaris, Roegneria Kamoji, Secale cereale or Thinopyrum baserabicum, and their alien chromosome stocks such as addition, substitution, translocation and introgression lines were developed via successive pollinations and immature embryo rescues. The resulted progenies were characterized using mitotic and meiotic analysis combining with chromosome banding and in situ hybridization. Isozyme pattern and RFLP analysis were used to determine the homoeologous relationship between alien chromosomes and those of common wheat. Irradiation, Ph system (homoeologous pairing control system), gametocidal chromosome effect were successfully employed to induce translocation and deletion lines. Chromosome C banding, in situ hybridization, RFLP and trait tracing were used to identify alien chromosome, chromosome segments, breakage point of translocation or deletion, and to map the introgressed genes such as those conferring resistance to powdery mildew, yellow rust, Fusarium head blight and take all diseases. As the result of above effort, new genetic materials, which contained multiple useful alien genes, were developed. To improve the agronomic characters of the derived lines, back crosses or “rolling” crosses using superior varieties or lines as recurrent parents are currently being conducted.展开更多
文摘mtDNAs of T type wheat cytoplasmic male sterile lines Ning Drawf 14(ND14) and their maintainers Ning Drawf 13 (ND13) were isolated and digested completely with restriction endonucleases EcoRI, PstI, EcoRV, BamHI. The results revealed that the molecular structure of mtDNAs from ND14 and ND13 cytoplasms were significantly deviated. The mitochondrial genomic difference between CMS line and maintainers were uncovered by southern hybridization with probes of 18S+5S rRNA、atpA genes from wheat and pea mitochondria, respectively. Due to the complexity of mtDNA and no proof of protein difference, it has not yet been demonstrated whether mtDNA difference of Normal and Male Sterile Cytoplasm of wheat is associated with CMS.
文摘Rich genetic resources are contained among wheat wild relatives. Cytogenetics Institute of Nanjing Agricultural University commences to transfer these genetic resources from relatives into common wheat. The hybrids between common wheat and Haynaldia villosa, Leymus racemosus,Roegneria ciliaris, Roegneria Kamoji, Secale cereale or Thinopyrum baserabicum, and their alien chromosome stocks such as addition, substitution, translocation and introgression lines were developed via successive pollinations and immature embryo rescues. The resulted progenies were characterized using mitotic and meiotic analysis combining with chromosome banding and in situ hybridization. Isozyme pattern and RFLP analysis were used to determine the homoeologous relationship between alien chromosomes and those of common wheat. Irradiation, Ph system (homoeologous pairing control system), gametocidal chromosome effect were successfully employed to induce translocation and deletion lines. Chromosome C banding, in situ hybridization, RFLP and trait tracing were used to identify alien chromosome, chromosome segments, breakage point of translocation or deletion, and to map the introgressed genes such as those conferring resistance to powdery mildew, yellow rust, Fusarium head blight and take all diseases. As the result of above effort, new genetic materials, which contained multiple useful alien genes, were developed. To improve the agronomic characters of the derived lines, back crosses or “rolling” crosses using superior varieties or lines as recurrent parents are currently being conducted.
