将荧光定量PCR技术与等位基因特异性扩增(Allele specific amplification,ASA)方法相结合,发展了一种可以快速检测基因点突变的实时荧光等位基因特异性扩增(Real-time ASA)方法.将该法用于检测K-ras癌基因第12位密码子发生的点突变,分...将荧光定量PCR技术与等位基因特异性扩增(Allele specific amplification,ASA)方法相结合,发展了一种可以快速检测基因点突变的实时荧光等位基因特异性扩增(Real-time ASA)方法.将该法用于检测K-ras癌基因第12位密码子发生的点突变,分别采用针对其不同点突变方式(GAT,GTT,CGT)设计的突变型引物对待测样品进行ASA,只有突变型样品能被顺利扩增出双链DNA产物,该产物才能与双链DNA染料SYBR GreenⅠ结合,发出荧光信号从而被检测到.用该法检测31例结肠癌组织中的K-ras癌基因点突变,其中有15例样品检出为突变型.Real-time ASA法可检测到样品中含量为1/1000的突变型基因,具有灵敏、快速、简便、安全、高通量和低成本等优点,可望用于大量临床样本的点突变筛查.展开更多
提出了一个新的利用延迟荧光强度来表征植物光合速率的系统。根据延迟荧光光谱特征,选用硅雪崩光电二极管(Si-APD),使其运行在盖革(Geiger)模式下作为延迟荧光单光子探测器,采用有源抑制和门控模式(AQGC)进行驱动,对温度、偏压、鉴别电...提出了一个新的利用延迟荧光强度来表征植物光合速率的系统。根据延迟荧光光谱特征,选用硅雪崩光电二极管(Si-APD),使其运行在盖革(Geiger)模式下作为延迟荧光单光子探测器,采用有源抑制和门控模式(AQGC)进行驱动,对温度、偏压、鉴别电平等参数进行了优化。为了验证系统性能,以大豆(Zao Shu No.18)叶片为模型样品进行了延迟荧光衰减动力学和本底的测量以及延迟荧光强度与最大净光合速率的对比测量,实验结果表明延迟荧光的信噪比达到1000:1,延迟荧光强度与最大净光合速率的线性相关度达到0.984。该系统具有准确和受外界环境影响小的优点。展开更多
The relation of the electrochemiluminescence(ECL) intensity with the shape of perforated and imperforated platinum electrodes has been investigated. ICCD(Intensified charge coupled device) was used to get the images o...The relation of the electrochemiluminescence(ECL) intensity with the shape of perforated and imperforated platinum electrodes has been investigated. ICCD(Intensified charge coupled device) was used to get the images of ECL of Ru(bpy) 3 Cl 2 ·6H 2 O and tripropylamine(TPA) on the electrodes. The results showed that the ECL was concentrated on the edge of the electrode. For the perforated platinum electrode, the ECL intensity was found to be dependent both on the electrode edge and the edge of the holes on the disk electrode. The ECL intensity of Ru(bpy) 3 Cl 2 ·6H 2 O measured on the four hole disk electrode was double higher than that measured on the simple disk electrode and the detection limit was increased approximately by three orders of magnitude from 1 fmol/L to 1 amol/L.展开更多
文摘提出了一个新的利用延迟荧光强度来表征植物光合速率的系统。根据延迟荧光光谱特征,选用硅雪崩光电二极管(Si-APD),使其运行在盖革(Geiger)模式下作为延迟荧光单光子探测器,采用有源抑制和门控模式(AQGC)进行驱动,对温度、偏压、鉴别电平等参数进行了优化。为了验证系统性能,以大豆(Zao Shu No.18)叶片为模型样品进行了延迟荧光衰减动力学和本底的测量以及延迟荧光强度与最大净光合速率的对比测量,实验结果表明延迟荧光的信噪比达到1000:1,延迟荧光强度与最大净光合速率的线性相关度达到0.984。该系统具有准确和受外界环境影响小的优点。
文摘The relation of the electrochemiluminescence(ECL) intensity with the shape of perforated and imperforated platinum electrodes has been investigated. ICCD(Intensified charge coupled device) was used to get the images of ECL of Ru(bpy) 3 Cl 2 ·6H 2 O and tripropylamine(TPA) on the electrodes. The results showed that the ECL was concentrated on the edge of the electrode. For the perforated platinum electrode, the ECL intensity was found to be dependent both on the electrode edge and the edge of the holes on the disk electrode. The ECL intensity of Ru(bpy) 3 Cl 2 ·6H 2 O measured on the four hole disk electrode was double higher than that measured on the simple disk electrode and the detection limit was increased approximately by three orders of magnitude from 1 fmol/L to 1 amol/L.
