Commercial Isomaltooligosaccharide was purified by glucose oxidase(GOD)and catalase(CAT).Temperature,pH value,sugar concentration and enzyme concentration were determined by uniform design and single factor experiment...Commercial Isomaltooligosaccharide was purified by glucose oxidase(GOD)and catalase(CAT).Temperature,pH value,sugar concentration and enzyme concentration were determined by uniform design and single factor experiment.The optimal condition was that:temperature was 35-40℃,pH value was about 4.0,sugar concentration was 200g/L,reaction time was 24h,GOD concenation was 100U/g sugar,the best ratio of GOD to CAT was 15.Under the optinal condition,the isomalto-oligosaccharide with a purity of 85.28% was obtained.展开更多
文摘Commercial Isomaltooligosaccharide was purified by glucose oxidase(GOD)and catalase(CAT).Temperature,pH value,sugar concentration and enzyme concentration were determined by uniform design and single factor experiment.The optimal condition was that:temperature was 35-40℃,pH value was about 4.0,sugar concentration was 200g/L,reaction time was 24h,GOD concenation was 100U/g sugar,the best ratio of GOD to CAT was 15.Under the optinal condition,the isomalto-oligosaccharide with a purity of 85.28% was obtained.
