采用毛细管电泳-电化学检测法(CE-ECD)同时测定了菟丝子中芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素等5种主要生物活性成分的含量,考察了运行缓冲液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离检测的影响。在最佳实验...采用毛细管电泳-电化学检测法(CE-ECD)同时测定了菟丝子中芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素等5种主要生物活性成分的含量,考察了运行缓冲液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离检测的影响。在最佳实验条件下,以直径300μm的碳圆盘电极为工作电极,检测电位为+950m V(vs.参比电极),以50mm o l/L的硼砂缓冲溶液(pH9.0)为运行缓冲液,上述各组分在19m in内能完全分离。芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素在两个数量级的范围内呈良好线性关系,检测下限(按S/N=3计)分别为1.93×10-5,3.55×10-4,3.65×10-5,1.73×10-5和1.46×10-4g/L。该法已成功地应用于菟丝子中活性成分的分离检测,结果令人满意。展开更多
The development of a novel electrochemical sensor for the detection of concentration of atmospheric sulfur dioxide in the low-mg/m 3 range is described. The gas sensor is based on a platinum microelectrode and a porou...The development of a novel electrochemical sensor for the detection of concentration of atmospheric sulfur dioxide in the low-mg/m 3 range is described. The gas sensor is based on a platinum microelectrode and a porous polymer film, which is directly connected with the gas-containing atmosphere. The gas sensor is very sensitive, selective, and reproducible for detection of SO2. The linear range over 0 10 mg/m 3 to 150 mg/m 3 and the detection limits is 0 05 mg/m 3.展开更多
文摘采用毛细管电泳-电化学检测法(CE-ECD)同时测定了菟丝子中芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素等5种主要生物活性成分的含量,考察了运行缓冲液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离检测的影响。在最佳实验条件下,以直径300μm的碳圆盘电极为工作电极,检测电位为+950m V(vs.参比电极),以50mm o l/L的硼砂缓冲溶液(pH9.0)为运行缓冲液,上述各组分在19m in内能完全分离。芦丁、金丝桃甙、山柰酚、对香豆酸和槲皮素在两个数量级的范围内呈良好线性关系,检测下限(按S/N=3计)分别为1.93×10-5,3.55×10-4,3.65×10-5,1.73×10-5和1.46×10-4g/L。该法已成功地应用于菟丝子中活性成分的分离检测,结果令人满意。
文摘The development of a novel electrochemical sensor for the detection of concentration of atmospheric sulfur dioxide in the low-mg/m 3 range is described. The gas sensor is based on a platinum microelectrode and a porous polymer film, which is directly connected with the gas-containing atmosphere. The gas sensor is very sensitive, selective, and reproducible for detection of SO2. The linear range over 0 10 mg/m 3 to 150 mg/m 3 and the detection limits is 0 05 mg/m 3.
