In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic re...In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.展开更多
目的：合成α_1-肾上腺素受体特异性激动剂——苯肾上腺素(phenylephrine，PE)荧光标记物，检测其药理学生物活性。方法：用化学合成的方法将绿色荧光染料 BODIPY-FL 和 PE 进行微量缩合反应，薄层色谱法分离和纯化，用薄层色谱和质谱...目的：合成α_1-肾上腺素受体特异性激动剂——苯肾上腺素(phenylephrine，PE)荧光标记物，检测其药理学生物活性。方法：用化学合成的方法将绿色荧光染料 BODIPY-FL 和 PE 进行微量缩合反应，薄层色谱法分离和纯化，用薄层色谱和质谱分析对其纯度和分子结构进行鉴定。通过分子生物学 Western blot 方法检测其药理学生物活性。结果：将 BODIPY-FL 与 PE 在无水、无氧和避光条件下进行羧合反应，经薄层色谱分离、纯化得到具有荧光的新化合物——BODIPY-FL-PE，结构经紫外光谱和质谱分析得到证实。Western blot 实验表明 BODIPY-FL-PE与 PE 同样具有激动α_1-肾上腺素受体，使胞外信号调节激酶(extracellular signal-regulated kinase，ERK)激活的药理作用。结论：经微量缩合反应合成的 BODIPY-FL-PE 具有α_1-肾上腺素受体激动剂药理学特性，可以用于肾上腺素受体行为观测，为进一步研究活细胞中肾上腺素受体的动态行为提供了工具。展开更多
目的:利用心肌肥大病理过程中基因表达谱的变化,构建基因/蛋白质调控网络。方法:以苯肾上腺素诱导新生大鼠心肌细胞肥大为模型,在分析肥大心肌细胞基因表达谱变化的基础上,进一步利用Pathwaystudio和Agilent Literature Search软件结合...目的:利用心肌肥大病理过程中基因表达谱的变化,构建基因/蛋白质调控网络。方法:以苯肾上腺素诱导新生大鼠心肌细胞肥大为模型,在分析肥大心肌细胞基因表达谱变化的基础上,进一步利用Pathwaystudio和Agilent Literature Search软件结合文献挖掘方法,构建基因/蛋白质相互作用网络。结果:构建的网络包含450个相互作用的基因/蛋白质(节点)以及592个它们之间相互作用的关系(边)。拓扑分析表明该网络具有无尺度特性,同时分析确定14个基因/蛋白质是网络的关键节点。通过GO(gene ontology)分析,发现在苯肾上腺素诱导新生大鼠心肌细胞肥大的过程中,与物质代谢、细胞信号转导及细胞骨架等密切相关的基因可能发挥了重要作用。结论:构建基因/蛋白质网络为研究心肌肥大的分子机制提供了有用的信息和方法。展开更多
文摘In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.
文摘目的：合成α_1-肾上腺素受体特异性激动剂——苯肾上腺素(phenylephrine，PE)荧光标记物，检测其药理学生物活性。方法：用化学合成的方法将绿色荧光染料 BODIPY-FL 和 PE 进行微量缩合反应，薄层色谱法分离和纯化，用薄层色谱和质谱分析对其纯度和分子结构进行鉴定。通过分子生物学 Western blot 方法检测其药理学生物活性。结果：将 BODIPY-FL 与 PE 在无水、无氧和避光条件下进行羧合反应，经薄层色谱分离、纯化得到具有荧光的新化合物——BODIPY-FL-PE，结构经紫外光谱和质谱分析得到证实。Western blot 实验表明 BODIPY-FL-PE与 PE 同样具有激动α_1-肾上腺素受体，使胞外信号调节激酶(extracellular signal-regulated kinase，ERK)激活的药理作用。结论：经微量缩合反应合成的 BODIPY-FL-PE 具有α_1-肾上腺素受体激动剂药理学特性，可以用于肾上腺素受体行为观测，为进一步研究活细胞中肾上腺素受体的动态行为提供了工具。
文摘目的:利用心肌肥大病理过程中基因表达谱的变化,构建基因/蛋白质调控网络。方法:以苯肾上腺素诱导新生大鼠心肌细胞肥大为模型,在分析肥大心肌细胞基因表达谱变化的基础上,进一步利用Pathwaystudio和Agilent Literature Search软件结合文献挖掘方法,构建基因/蛋白质相互作用网络。结果:构建的网络包含450个相互作用的基因/蛋白质(节点)以及592个它们之间相互作用的关系(边)。拓扑分析表明该网络具有无尺度特性,同时分析确定14个基因/蛋白质是网络的关键节点。通过GO(gene ontology)分析,发现在苯肾上腺素诱导新生大鼠心肌细胞肥大的过程中,与物质代谢、细胞信号转导及细胞骨架等密切相关的基因可能发挥了重要作用。结论:构建基因/蛋白质网络为研究心肌肥大的分子机制提供了有用的信息和方法。
