目的 基于现代科学技术对四妙勇安汤进行改良,验证其促进糖尿病足伤口愈合的效果。方法 采用乙氧二羟丁酮辅助的单链DNA测序(kethoxal-assisted single stranded DNA sequencing, KAS-seq)技术检测糖尿病足患者创面组织和正常创面组织,...目的 基于现代科学技术对四妙勇安汤进行改良,验证其促进糖尿病足伤口愈合的效果。方法 采用乙氧二羟丁酮辅助的单链DNA测序(kethoxal-assisted single stranded DNA sequencing, KAS-seq)技术检测糖尿病足患者创面组织和正常创面组织,筛选糖尿病足相关的关键基因,并结合网络药理学,根据这些关键基因寻找有针对性的中药从而对四妙勇安汤进行改良,最后通过糖尿病足动物模型验证改良方的治疗效果。结果 通过临床样本测序分析得到CREB1、GRID2、PIK3R3、DRD2、ADORA1、TNFSF11、EDNRB、HTR1B 8个关键基因,靶向到黄柏、苍术、地黄和半夏4味中药配伍入原方。在灌胃干预第7天时改良方组(S+)小鼠伤口愈合效率显著高于对照组(Control)(P<0.05),第12天时S+组伤口愈合率显著高于原方组(S)(P<0.05)。改良方中药靶点在原方基础上更多富集到了神经系统、缺氧和血液循环调节相关通路中。结论 改良方与经方相比具有更好地促进糖尿病足伤口愈合的效果,相关机制可能与调节炎性反应、改善周围神经病变和促进伤口愈合等通路有关,同时也为中药方剂的临床加减应用提供了一种新方法和现代科学理论依据。展开更多
To evaluate the safety of oral cabamazepine during breast milk feeding.The carbamazepine concentration in breast milk of one epilepsy maternal patient was assayed by high performance liquid chromatography,and the lite...To evaluate the safety of oral cabamazepine during breast milk feeding.The carbamazepine concentration in breast milk of one epilepsy maternal patient was assayed by high performance liquid chromatography,and the literature was reviewed to find the nursing evidence in the use of cabamazepine.The carbamazepine concentration in breast milk ranged from 0.34-0.86 mg/L.The neonate daily dose intake was estimated ranging from 0.34 mg to 0.86 mg through breast-feeding in theory.The literature showed that carbamazepine was generally considered safe for use during breast feeding;however,adverse effects should be monitored as recommended.It is better to avoid feeding at high concentration level to minimize the harm of carbamazepine to the baby.展开更多
文摘To evaluate the safety of oral cabamazepine during breast milk feeding.The carbamazepine concentration in breast milk of one epilepsy maternal patient was assayed by high performance liquid chromatography,and the literature was reviewed to find the nursing evidence in the use of cabamazepine.The carbamazepine concentration in breast milk ranged from 0.34-0.86 mg/L.The neonate daily dose intake was estimated ranging from 0.34 mg to 0.86 mg through breast-feeding in theory.The literature showed that carbamazepine was generally considered safe for use during breast feeding;however,adverse effects should be monitored as recommended.It is better to avoid feeding at high concentration level to minimize the harm of carbamazepine to the baby.
