面对日益增长的新病毒种类和数量,及其复杂的内在关系,现有的病毒分类方法已凸显出其局限性。2019年,病毒分类和命名的权威机构国际病毒分类委员会(the International Committee on Taxonomy of Viruses,ICTV),基于病毒的系统进化关系...面对日益增长的新病毒种类和数量,及其复杂的内在关系,现有的病毒分类方法已凸显出其局限性。2019年,病毒分类和命名的权威机构国际病毒分类委员会(the International Committee on Taxonomy of Viruses,ICTV),基于病毒的系统进化关系提出了一种全新的、包含15个分类等级或阶元的病毒分类系统,并已经在ICTV的官方网站https://ictv.global/上线发布,公众可以在线免费查找动态的病毒分类信息。新的病毒分类系统能更好地揭示病毒错综复杂的内在关系以及群体进化机制,使我们能以更客观及系统的方法对病毒进行分类和命名。本文就ICTV网站有关病毒分类的主要概况,特别是最新的病毒分类和ICTV报告进展作一综述。展开更多
An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical an...An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical and epidemiological char acte ristics: 86.4% cases occurred in the age group of 20-59 years; with male prepon d erance and autumn-winter excess; 75% patients had a history of contact with hep a titis cases, and / or eating out or drinking unboiled water; clinical features w ere s imilar to hepatitis A, with a higher fatality rate, especially in pregnant women (up to 21%). Two strains of hepatitis E virus (HEV) isolated from Xinjiang were completely seq uenced by dideoxy chain termina tion method, with nucleotide homology of 93.5% and 94.5% with Burma strain (geno type 1), 75.7% and 75.9% with Mexico strain (genotype 2), 73.7% and 74.2 % with American strain (genotype 3), respectively. It suggests that these two st rains belong to genotype 1. The partial sequencing of open reading frame (ORF)2 region of 98 HEV isol ates from 19 cities of China revealed that 62 of them (63.3%) shared the same ge notype with Burma and Xinjiang strains, and 36 (36.7%) isolates were of genotype 4. One of the 36 strains was completely sequenced, with nucleotide identity of 75% with Burma strain, 74.5% with Mexico strain and 75.3% with American strain , respectively. Sera collected from 419 pigs, 190 cattle and 316 goats from var ious regions of China were detected for anti-HEV antibodies and HEV RNA using a n in-house enzyme immunoassay (EIA) and reverse transcriptase nested polymerase chain re action (RT-nPCR) with primers from ORF 2. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6. 3%, respectively, but none of the goat sera was anti-HEV positive. The PCR product s (nt 6007- 6354) of 5 HEV RNA positive sera were sequenced and compared to othe r HEV sequences in the nucleotide databases. The five sequences shared 74%-79%, 73%-74%, 73%-78%, and 83%-99% identity to HEV genotypes 1,2,3 and 4, respe c tively. An enzyme immunoassay (EIA) and Western immunobloting (WB) for detection of anti-HEV and a reverse transcriptase nested polymerase chain reaction (RT- nPCR) of HEV RNA were developed. The anti-HEV EIA kit was approved by the State Drug Administration, China. An experimental model of HEV was established in rhe sus monkeys (Macaca mulatta). The animal studies on HEV cDNA vaccine and recombi nant vaccine showed that the humoral immune response to HEV was induced in mice after inoculation of the HEV RNA vaccine, and the HEV recombinant vaccine provid ed a protection from HEV infection in Rhesus monkeys.展开更多
文摘面对日益增长的新病毒种类和数量,及其复杂的内在关系,现有的病毒分类方法已凸显出其局限性。2019年,病毒分类和命名的权威机构国际病毒分类委员会(the International Committee on Taxonomy of Viruses,ICTV),基于病毒的系统进化关系提出了一种全新的、包含15个分类等级或阶元的病毒分类系统,并已经在ICTV的官方网站https://ictv.global/上线发布,公众可以在线免费查找动态的病毒分类信息。新的病毒分类系统能更好地揭示病毒错综复杂的内在关系以及群体进化机制,使我们能以更客观及系统的方法对病毒进行分类和命名。本文就ICTV网站有关病毒分类的主要概况,特别是最新的病毒分类和ICTV报告进展作一综述。
文摘An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical and epidemiological char acte ristics: 86.4% cases occurred in the age group of 20-59 years; with male prepon d erance and autumn-winter excess; 75% patients had a history of contact with hep a titis cases, and / or eating out or drinking unboiled water; clinical features w ere s imilar to hepatitis A, with a higher fatality rate, especially in pregnant women (up to 21%). Two strains of hepatitis E virus (HEV) isolated from Xinjiang were completely seq uenced by dideoxy chain termina tion method, with nucleotide homology of 93.5% and 94.5% with Burma strain (geno type 1), 75.7% and 75.9% with Mexico strain (genotype 2), 73.7% and 74.2 % with American strain (genotype 3), respectively. It suggests that these two st rains belong to genotype 1. The partial sequencing of open reading frame (ORF)2 region of 98 HEV isol ates from 19 cities of China revealed that 62 of them (63.3%) shared the same ge notype with Burma and Xinjiang strains, and 36 (36.7%) isolates were of genotype 4. One of the 36 strains was completely sequenced, with nucleotide identity of 75% with Burma strain, 74.5% with Mexico strain and 75.3% with American strain , respectively. Sera collected from 419 pigs, 190 cattle and 316 goats from var ious regions of China were detected for anti-HEV antibodies and HEV RNA using a n in-house enzyme immunoassay (EIA) and reverse transcriptase nested polymerase chain re action (RT-nPCR) with primers from ORF 2. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6. 3%, respectively, but none of the goat sera was anti-HEV positive. The PCR product s (nt 6007- 6354) of 5 HEV RNA positive sera were sequenced and compared to othe r HEV sequences in the nucleotide databases. The five sequences shared 74%-79%, 73%-74%, 73%-78%, and 83%-99% identity to HEV genotypes 1,2,3 and 4, respe c tively. An enzyme immunoassay (EIA) and Western immunobloting (WB) for detection of anti-HEV and a reverse transcriptase nested polymerase chain reaction (RT- nPCR) of HEV RNA were developed. The anti-HEV EIA kit was approved by the State Drug Administration, China. An experimental model of HEV was established in rhe sus monkeys (Macaca mulatta). The animal studies on HEV cDNA vaccine and recombi nant vaccine showed that the humoral immune response to HEV was induced in mice after inoculation of the HEV RNA vaccine, and the HEV recombinant vaccine provid ed a protection from HEV infection in Rhesus monkeys.
