ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. I...ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. It has been used widely as an endothelial cell model and an useful re search tool in biomedicine and pharmacology. However, several distinct differenc es exist between ECV304 and HUVEC. Some studies even pointed out that ECV304 is not of HUVEC origin. According to the research data including ours, this reporte dly endothelial-derived permanent human cell line ECV304 may be dedifferentiated towards an epithelial phenotype. It is therefore not an appropriate cell line t o study endothelial cell biology. But cultured ECV304 cells can still be used as a model, tool or target in the pathophysiological and pharmacological studies, depending on whether or not their functional expression or markers are suitable for the research work.展开更多
基金国家自然科学基金青年基金项目“基于本体方法的我国上市甲乙肝疫苗相关不良反应数据挖掘与建模分析”(61801067)重庆市教育委员会科学技术研究重点项目“卡介苗两种用途下相应不良反应信息的本体化表征与建模分析”(KJZD-K202000603)+4 种基金中国医学科学院中央级公益性科研院所基本科研业务费专项资金资助,“国家卫生健康委员会肺脏免疫性疾病诊治重点实验室”建设项目(2019PT320003)University of Michigan Medical School Global Reach FundMichigan Medicine-Peking University Health Sciences Center Joint Institute for Clinical and Translational Research“Systemic Investigation of the Microbiome-host interactions in H.pylori-associated Gastric Cancer Patients”(U063430,BMU2019JI010)国家重点研发计划精准医学研究专项“疾病表型组-实验组学数据分析、注释与整合”(2017YFC0908404)中国医学科学院医学与健康科技创新工程项目“生物医学本体支持的通用数据元素表示和应用系统建设”(2018-I2M-AI-009)。
文摘ECV304 was reported first in 1990 as a spont aneously-transformed and immortalized cell line derived from a Japanese HUVEC. S ubsequently, many studies validated that the ECV304 is a permanent endothelial cell line. It has been used widely as an endothelial cell model and an useful re search tool in biomedicine and pharmacology. However, several distinct differenc es exist between ECV304 and HUVEC. Some studies even pointed out that ECV304 is not of HUVEC origin. According to the research data including ours, this reporte dly endothelial-derived permanent human cell line ECV304 may be dedifferentiated towards an epithelial phenotype. It is therefore not an appropriate cell line t o study endothelial cell biology. But cultured ECV304 cells can still be used as a model, tool or target in the pathophysiological and pharmacological studies, depending on whether or not their functional expression or markers are suitable for the research work.
