A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using ...A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.展开更多
A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crys...A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al . on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable.展开更多
基金The Research Grants Council of the Hong Kong Special Adm inistration RegionChina(No.HKBU 2 0 5 4/98p) +1 种基金国家杰出青年基金 (批准号 :2 982 5 110 )湖南省自然科学基金 (批准号 :0 0 GKY10 10 )资助
文摘A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.
文摘A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al . on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable.
