Glutaraldehyde crosslinked chitosan used in the adsorption upon model uremic middle molecular toxins was studied. In comparison with untreated glutaraldehyde crosslinked chitosan resin beads, the hydrogen reduced ones...Glutaraldehyde crosslinked chitosan used in the adsorption upon model uremic middle molecular toxins was studied. In comparison with untreated glutaraldehyde crosslinked chitosan resin beads, the hydrogen reduced ones showed a quite realistic behavior in the adsorption upon those model toxins, and the amount of adsorption was fairly high while the equilibrium time was obviously shortened.展开更多
Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peak...Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic serum and urine were less than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 8 to 9 subpeaks at 230 nm by anion exchange chromatography. One of these subpeaks, A 3, was detected in uremic serum and normal urine but undetectable in uremic urine. After a gel permeation chromatography with bidistilled water as eluent for desalting, subfraction A 3 was seperated into two parts designated A 3 Ⅰ and A 3 Ⅱ in order. The results of MALDI TOF MS revealed that the two peaks from both samples were identical respectively, fraction A 3 Ⅰ contained three kinds of components with molecular weight 839.69, 1 007.94 and 2 015.16 and fraction A 3 Ⅱ consisted of other three kinds of components with molecular weight 873.69, 1 106.67 and 1 680.28.展开更多
Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular pe...Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular peaks（A and B） were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight {1 214}, which could hardly be seen in the fraction A-Ⅰ of uremic urine.展开更多
文摘Glutaraldehyde crosslinked chitosan used in the adsorption upon model uremic middle molecular toxins was studied. In comparison with untreated glutaraldehyde crosslinked chitosan resin beads, the hydrogen reduced ones showed a quite realistic behavior in the adsorption upon those model toxins, and the amount of adsorption was fairly high while the equilibrium time was obviously shortened.
文摘Sera and urine from patients with severe uremia and healthy subjects were seperated by means of gel permeation chromatography on Sephadex G15 column with N(C 2H 5) 3 H 2CO 3 buffer as eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic serum and urine were less than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 8 to 9 subpeaks at 230 nm by anion exchange chromatography. One of these subpeaks, A 3, was detected in uremic serum and normal urine but undetectable in uremic urine. After a gel permeation chromatography with bidistilled water as eluent for desalting, subfraction A 3 was seperated into two parts designated A 3 Ⅰ and A 3 Ⅱ in order. The results of MALDI TOF MS revealed that the two peaks from both samples were identical respectively, fraction A 3 Ⅰ contained three kinds of components with molecular weight 839.69, 1 007.94 and 2 015.16 and fraction A 3 Ⅱ consisted of other three kinds of components with molecular weight 873.69, 1 106.67 and 1 680.28.
文摘Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N（C2H5）3-H2CO3 buffer as the eluent. Two middle molecular peaks（A and B） were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight {1 214}, which could hardly be seen in the fraction A-Ⅰ of uremic urine.
