In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H 2O 2/p-iodophenol(P...In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H 2O 2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab 2-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.展开更多
A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-comp...A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-competitive immunoassay procedure,seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by（p-iodophenol）.The detection limit（S/N=3） of AFP is 0.85 μg/L.The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera,which coincides with the results detected by Beckman automatic CL analyzer-access IA system.展开更多
A new method for sensitive and rapid indirect electrochemiluminescence(IECL)detection of norepinephrine(NE),synephrine,and isoproterenol with capillary electrophoresis(CE)was developed.IECL detection is based on their...A new method for sensitive and rapid indirect electrochemiluminescence(IECL)detection of norepinephrine(NE),synephrine,and isoproterenol with capillary electrophoresis(CE)was developed.IECL detection is based on their quenching effects on Ru(bpy)2+3/tripropylamine(TPA)system.Under the optimum conditions,the detection limits(S/N=3)were 2.6×10-8 mol/L for NE,6.6×10-9 mol/L for synephrine,and 8.4×10-8 mol/L for isoproterenol.The RSD of migration time and peak area were less than 1.3% and 4.4%,respectively.The proposed method was successfully applied to the analysis of NE in the urine samples of 10 healthy people and 10 diabetic nephropathy patients.The results indicate that the content of NE in diabetic nephropathy patients is much higher than that in healthy people.展开更多
A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,...A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,BMIMBF4). The conditions for the CE separation,ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions,the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME,thebaine,CP,and AC were 2.1×10-8,1.4×10-7,6.3×10-8,and 3.6×10-8 mol/L(S/N=3),respectively. The limits of quantitation(LOQ,S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME,3.6 ×10-6 mol/L for thebaine,6.5×10-7 mol/L for CP,and 4.6×10-7 mol/L for AC,respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.展开更多
文摘In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H 2O 2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab 2-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.
文摘A novel detection method of a tumor marker alpha-fetoprotein（AFP） in human sera via capillary（electrophoresis） immunoassay coupled with chemiluminescence detection（CEIACL） was established.After a simple non-competitive immunoassay procedure,seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H2O2 catalyzed by horseradish peroxidase and enhanced by（p-iodophenol）.The detection limit（S/N=3） of AFP is 0.85 μg/L.The CEIACL method proposed was successfully applied to the sensitive detection of AFP in human sera,which coincides with the results detected by Beckman automatic CL analyzer-access IA system.
文摘A new method for sensitive and rapid indirect electrochemiluminescence(IECL)detection of norepinephrine(NE),synephrine,and isoproterenol with capillary electrophoresis(CE)was developed.IECL detection is based on their quenching effects on Ru(bpy)2+3/tripropylamine(TPA)system.Under the optimum conditions,the detection limits(S/N=3)were 2.6×10-8 mol/L for NE,6.6×10-9 mol/L for synephrine,and 8.4×10-8 mol/L for isoproterenol.The RSD of migration time and peak area were less than 1.3% and 4.4%,respectively.The proposed method was successfully applied to the analysis of NE in the urine samples of 10 healthy people and 10 diabetic nephropathy patients.The results indicate that the content of NE in diabetic nephropathy patients is much higher than that in healthy people.
文摘A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,BMIMBF4). The conditions for the CE separation,ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions,the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME,thebaine,CP,and AC were 2.1×10-8,1.4×10-7,6.3×10-8,and 3.6×10-8 mol/L(S/N=3),respectively. The limits of quantitation(LOQ,S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME,3.6 ×10-6 mol/L for thebaine,6.5×10-7 mol/L for CP,and 4.6×10-7 mol/L for AC,respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.
