Derivation conditions of pre-column derivation-HPLC method used for determinating artemisinin content were compared and selected,and artemisinin content in above-ground part of Artemisia annua L.from seventeen locatio...Derivation conditions of pre-column derivation-HPLC method used for determinating artemisinin content were compared and selected,and artemisinin content in above-ground part of Artemisia annua L.from seventeen locations was compared by optimal pre-column derivation-HPLC method.The optimal derivation condition is selected via comparing of 0.2% NaOH solution addition(3,4,5,6 and 7 mL),derivation temperature(30 ℃,35 ℃,40 ℃,45 ℃ and 50 ℃) and derivation time(0,2,5,10,20,40 and 60 min) during derivation process.The optimal derivation condition is adding 5 mL 0.2% NaOH solution,then insulating in 40 ℃ water bath for 10 min.Differences of artemisinin content in A.annua from seventeen locations are obvious,the general trend is that artemisinin content decreases gradually with location from south to north,in which artemisinin content in A.annua from Youyang of Chongqing is the highest with a value of 7.08 mg·g-1.The method is simple and accurate with good reproducibility,and can be used to determinate artemisinin content in medicinal material of A.annua.展开更多
Thirteen compounds were isolated from dry above-ground part of Scutellaria barbata D.Don and their structures were identified.These compounds are p-hydroxybenzaldehyde(Ⅰ),p-coumaric acid(Ⅱ),hydroxyhydroquinone(Ⅲ),h...Thirteen compounds were isolated from dry above-ground part of Scutellaria barbata D.Don and their structures were identified.These compounds are p-hydroxybenzaldehyde(Ⅰ),p-coumaric acid(Ⅱ),hydroxyhydroquinone(Ⅲ),hispidulin(IV),5,7,4′-trihydroxy-8-methoxyflavone(Ⅴ),apigenin(Ⅵ),luteolin(Ⅶ),apigenin-5-O-β-D-glucopyranoside(Ⅷ),scutellaric acid(Ⅸ),stigmasta-5,22-dien-3-O-β-D-glucopyranoside(Ⅹ),barbatin C(Ⅺ),β-sito-sterol(Ⅻ) and daucosterol(ⅩⅢ).Compound Ⅷ is isolated from Scutellaria L.for the first time,and compound Ⅲ and compound X are isolated from S.barbata for the first time.展开更多
文摘Derivation conditions of pre-column derivation-HPLC method used for determinating artemisinin content were compared and selected,and artemisinin content in above-ground part of Artemisia annua L.from seventeen locations was compared by optimal pre-column derivation-HPLC method.The optimal derivation condition is selected via comparing of 0.2% NaOH solution addition(3,4,5,6 and 7 mL),derivation temperature(30 ℃,35 ℃,40 ℃,45 ℃ and 50 ℃) and derivation time(0,2,5,10,20,40 and 60 min) during derivation process.The optimal derivation condition is adding 5 mL 0.2% NaOH solution,then insulating in 40 ℃ water bath for 10 min.Differences of artemisinin content in A.annua from seventeen locations are obvious,the general trend is that artemisinin content decreases gradually with location from south to north,in which artemisinin content in A.annua from Youyang of Chongqing is the highest with a value of 7.08 mg·g-1.The method is simple and accurate with good reproducibility,and can be used to determinate artemisinin content in medicinal material of A.annua.
文摘Thirteen compounds were isolated from dry above-ground part of Scutellaria barbata D.Don and their structures were identified.These compounds are p-hydroxybenzaldehyde(Ⅰ),p-coumaric acid(Ⅱ),hydroxyhydroquinone(Ⅲ),hispidulin(IV),5,7,4′-trihydroxy-8-methoxyflavone(Ⅴ),apigenin(Ⅵ),luteolin(Ⅶ),apigenin-5-O-β-D-glucopyranoside(Ⅷ),scutellaric acid(Ⅸ),stigmasta-5,22-dien-3-O-β-D-glucopyranoside(Ⅹ),barbatin C(Ⅺ),β-sito-sterol(Ⅻ) and daucosterol(ⅩⅢ).Compound Ⅷ is isolated from Scutellaria L.for the first time,and compound Ⅲ and compound X are isolated from S.barbata for the first time.
