The elucidation of key influence factors for electrostatic adsorption is very important to control protein nonspecific adsorption on modified surfaces. In this study, real time surface plasmon resonance technique is u...The elucidation of key influence factors for electrostatic adsorption is very important to control protein nonspecific adsorption on modified surfaces. In this study, real time surface plasmon resonance technique is used to characterize the electrostatic adsorption of two proteins (mouse IgG and protein A) on carboxymethyldextran modified surface. The results show that protein solution pH and ionic strength are key influence factors for efficient electrostatic adsorption. The influence of protein solution pH on the amount of electrostatic adsorption depends on the type of the charge and the charge density of both protein and modified matrix on the surface. The electrostatic adsorption process involves a competition between the positively charged protein and other positively charged species in the buffer solution. A decrease of ionic strength leads to an increasing electrostatic adsorption. The kinetic adsorption constants of protein A at different pH values were also calculated and compared.展开更多
Manipulation of electroosmotic flow in capillary electrophoresis is an importantstep for separation of inorganic anions.The type and concentratlon of electroosmotic flowmodifier (OFM) exert a tremendous influence on t...Manipulation of electroosmotic flow in capillary electrophoresis is an importantstep for separation of inorganic anions.The type and concentratlon of electroosmotic flowmodifier (OFM) exert a tremendous influence on the electroosmotic mobllity. ln the pres-ence of CTAB as buffer solutions’ pH values increase, the electroosmotic mobility becomeslower. At the same ionic strength, the buffer type affects the electroosmotic veloclty and themigration order for iodide and iodate.展开更多
文摘The elucidation of key influence factors for electrostatic adsorption is very important to control protein nonspecific adsorption on modified surfaces. In this study, real time surface plasmon resonance technique is used to characterize the electrostatic adsorption of two proteins (mouse IgG and protein A) on carboxymethyldextran modified surface. The results show that protein solution pH and ionic strength are key influence factors for efficient electrostatic adsorption. The influence of protein solution pH on the amount of electrostatic adsorption depends on the type of the charge and the charge density of both protein and modified matrix on the surface. The electrostatic adsorption process involves a competition between the positively charged protein and other positively charged species in the buffer solution. A decrease of ionic strength leads to an increasing electrostatic adsorption. The kinetic adsorption constants of protein A at different pH values were also calculated and compared.
文摘Manipulation of electroosmotic flow in capillary electrophoresis is an importantstep for separation of inorganic anions.The type and concentratlon of electroosmotic flowmodifier (OFM) exert a tremendous influence on the electroosmotic mobllity. ln the pres-ence of CTAB as buffer solutions’ pH values increase, the electroosmotic mobility becomeslower. At the same ionic strength, the buffer type affects the electroosmotic veloclty and themigration order for iodide and iodate.
