Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with ...Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.展开更多
Traditional Chinese Medicine (TCM) based on natural products is one important part of the Chinese civilization. Owing to the complexity of the composition, the study on medical effective components and curative effect...Traditional Chinese Medicine (TCM) based on natural products is one important part of the Chinese civilization. Owing to the complexity of the composition, the study on medical effective components and curative effects are very difficult. Fuzi (Radix Aconiti Lateralis Praeparata) has been widely used for cardiotonic and analgesics in China,however, the component-aconitine in which is very toxic and may cause some side-effect.This paper reported the application of electrospray iohization mass spectrometric(ESIMS)technique on study of the compound prescriptions containing fuzi, Renshensini concoction and Baweidihuang concoction, to explore the mechanism of synergy between fuzi and other herbs. From chemical point of view, the detoxification mechanism can be attributed to the hydrolysis of the solubility of toxic diester-alkaloids from concoctions of fuzi. Radix Glycyrrhizae Praeparata, Rhizoma Zingiberis, Radix Ginsengp promote the hydrolysis to produce the less toxic monoester-alkaloids and Fructus Corni reduce the solubility of hypa-conitine from fuzi. However pinellia fritillary and bletilla were forbidden to use with fuzi,because they suppress the hydrolysis of fuzi in the solution. This work applied electrospray ionization mass spectrometry, tandem mass spectrometry (MSn) , high performance liquid chromatography (HPLC) techniques to study the reactions (including hydrolysis and ester-exchange reaction etc. ) occurred during the decoction and the concoction process via the gas phase ion chemical reactions and discuss the conditions to the reactions. On the other hand, the fragmentation mechanism of aconitine alkaloids in mass spectrometry was studied as well.展开更多
Electrospray ionization tandem mass spectrometry (ESI-MS^n) was used to char-acterize the non-covalent complexes between baicalin and α-, β-, γ-cyclodextrins (CDs).α-,β-, γ-CDs and baicalin form the 1 ： 1 and 2...Electrospray ionization tandem mass spectrometry (ESI-MS^n) was used to char-acterize the non-covalent complexes between baicalin and α-, β-, γ-cyclodextrins (CDs).α-,β-, γ-CDs and baicalin form the 1 ： 1 and 2 ： 1 complexes. The relative stability of complexes was studied using ESI-MS^n.展开更多
A monoterpene lactone, loliolide, was separated from ornithogalum caudatum and its structure was identified by NMR and electrospay ionization tandem mass spectrometry (ESI-MS^n). To the best of our knowledge, loliolid...A monoterpene lactone, loliolide, was separated from ornithogalum caudatum and its structure was identified by NMR and electrospay ionization tandem mass spectrometry (ESI-MS^n). To the best of our knowledge, loliolide was first separated from the genus Ornithogalum.展开更多
A novel kind of alkaloid was isolated from the aerial of Epimedium koreanum Naki. The molecular weight and molecular formula were achieved by FT-ICRMS as 370 128 5012 and C 20H 20O 6N. The molecular structure of the c...A novel kind of alkaloid was isolated from the aerial of Epimedium koreanum Naki. The molecular weight and molecular formula were achieved by FT-ICRMS as 370 128 5012 and C 20H 20O 6N. The molecular structure of the compound C 20H 20O 6N was determined on the basis of spectral analyses as 6-hydroxy-11,12-dimethoxy-2,2-dimethyl-1,8-dioxo-1,3,4,8-tetrahydro-2H-7-oxa-2-azonia-benzo phenanthene. Using electrospray ionization multi-stage mass spectrometry, the structure was confirmed further. The compound was first reported from this kind of plant.展开更多
The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cell...The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.展开更多
In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic re...In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.展开更多
The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One pr...The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein（sorcin）. Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.展开更多
Two flavonol glycoside isomers of Icariside I and Caohuoside C were differentiated by electrospray ionization tandem mass spectrometry (ESI-MS^n) method. In the negative ion mode, the two compounds with different glyc...Two flavonol glycoside isomers of Icariside I and Caohuoside C were differentiated by electrospray ionization tandem mass spectrometry (ESI-MS^n) method. In the negative ion mode, the two compounds with different glycosyl moieties showed the same ESI-MS spectrum, but obvious different MS2 spectra and thus they can be distinguished rapidly, accurately and easily by ESI-MS.展开更多
文摘Oligodeoxynucleotide from SARS virus was selected and synthesized. Its complexes with alkaloid compounds were investigated by electrospray mass spectrometry. We found that three alkaloid compounds could interact with the DNA target molecule in five alkaloids analyzed. With increasing molar ratio of the three alkaloid compounds, the complexes between alkaloids and DNA with different stoichiometric ratios were found in their MS spectra. The order of the alkalinity of their gas phase corresponds to that of liquid phase, that is: Palmatine>Jatrorrhizine>Berberin. The relative abundance of these complexes in mass spectra can assess the relative affinities of above three alkaloid compounds with the DNA target molecule. In this paper, the binding sites between jatrorrhizine and DNA was deduced from the interaction experiments of jatrorrhizine with three nucleosides.
文摘Traditional Chinese Medicine (TCM) based on natural products is one important part of the Chinese civilization. Owing to the complexity of the composition, the study on medical effective components and curative effects are very difficult. Fuzi (Radix Aconiti Lateralis Praeparata) has been widely used for cardiotonic and analgesics in China,however, the component-aconitine in which is very toxic and may cause some side-effect.This paper reported the application of electrospray iohization mass spectrometric(ESIMS)technique on study of the compound prescriptions containing fuzi, Renshensini concoction and Baweidihuang concoction, to explore the mechanism of synergy between fuzi and other herbs. From chemical point of view, the detoxification mechanism can be attributed to the hydrolysis of the solubility of toxic diester-alkaloids from concoctions of fuzi. Radix Glycyrrhizae Praeparata, Rhizoma Zingiberis, Radix Ginsengp promote the hydrolysis to produce the less toxic monoester-alkaloids and Fructus Corni reduce the solubility of hypa-conitine from fuzi. However pinellia fritillary and bletilla were forbidden to use with fuzi,because they suppress the hydrolysis of fuzi in the solution. This work applied electrospray ionization mass spectrometry, tandem mass spectrometry (MSn) , high performance liquid chromatography (HPLC) techniques to study the reactions (including hydrolysis and ester-exchange reaction etc. ) occurred during the decoction and the concoction process via the gas phase ion chemical reactions and discuss the conditions to the reactions. On the other hand, the fragmentation mechanism of aconitine alkaloids in mass spectrometry was studied as well.
文摘Electrospray ionization tandem mass spectrometry (ESI-MS^n) was used to char-acterize the non-covalent complexes between baicalin and α-, β-, γ-cyclodextrins (CDs).α-,β-, γ-CDs and baicalin form the 1 ： 1 and 2 ： 1 complexes. The relative stability of complexes was studied using ESI-MS^n.
文摘A monoterpene lactone, loliolide, was separated from ornithogalum caudatum and its structure was identified by NMR and electrospay ionization tandem mass spectrometry (ESI-MS^n). To the best of our knowledge, loliolide was first separated from the genus Ornithogalum.
文摘A novel kind of alkaloid was isolated from the aerial of Epimedium koreanum Naki. The molecular weight and molecular formula were achieved by FT-ICRMS as 370 128 5012 and C 20H 20O 6N. The molecular structure of the compound C 20H 20O 6N was determined on the basis of spectral analyses as 6-hydroxy-11,12-dimethoxy-2,2-dimethyl-1,8-dioxo-1,3,4,8-tetrahydro-2H-7-oxa-2-azonia-benzo phenanthene. Using electrospray ionization multi-stage mass spectrometry, the structure was confirmed further. The compound was first reported from this kind of plant.
文摘The proteomic maps of human ovarian adenocarcinoma cell line in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE.The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment.Approximate 1 300 protein spots were resolved in the two ranges of pI（3—10）.The spots which have 10-fold difference in light density were digested by in-gel（enzyme）,identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and finally detected by Profound and Mascot search engine in IDI databases.Seven proteins with obviously altered abundances were detected specifically,including four up-regulated protein spots and three down-regulated protein spots.The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the therapy and pathogenesis.
文摘In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.
文摘The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein（sorcin）. Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.
文摘Two flavonol glycoside isomers of Icariside I and Caohuoside C were differentiated by electrospray ionization tandem mass spectrometry (ESI-MS^n) method. In the negative ion mode, the two compounds with different glycosyl moieties showed the same ESI-MS spectrum, but obvious different MS2 spectra and thus they can be distinguished rapidly, accurately and easily by ESI-MS.
