运用PCR产物直接测序的方法对海南省文昌市90只文昌鸡样品的线粒体DNA控制区(mtDNA D-loop)第Ⅰ高变区序列进行了分析。用CLUSTAL X 1.81软件进行序列比较,其中38只个体共检测到27个核苷酸多态位点,全部为转换无颠换,序列突变率为5.29%...运用PCR产物直接测序的方法对海南省文昌市90只文昌鸡样品的线粒体DNA控制区(mtDNA D-loop)第Ⅰ高变区序列进行了分析。用CLUSTAL X 1.81软件进行序列比较,其中38只个体共检测到27个核苷酸多态位点,全部为转换无颠换,序列突变率为5.29%。用DnaSP软件估计出序列存在16个单倍型,单倍型多样度(Hd)为0.935,核苷酸多样度(π)为0.01479,平均核苷酸差异数(k)为7.541。运用Mega4.0软件计算出不同个体间的遗传距离,并据此构建了NJ系统树。文昌鸡样品在NJ无根树上聚为4大分支。研究结果表明,文昌鸡群体内个体序列变异程度较大,遗传多样性丰富,揭示文昌鸡在遗传组成上具有4个母系来源。展开更多
The genus Simocephalus Schoedler 1858,a large,nonpelagic daphniidae,are abundant in the littoral zone,expecially with submerged aquatic vegetation in freshwater bodies all over the world.More than 63 specific and subs...The genus Simocephalus Schoedler 1858,a large,nonpelagic daphniidae,are abundant in the littoral zone,expecially with submerged aquatic vegetation in freshwater bodies all over the world.More than 63 specific and subspecific names have been proposed.But in the recent research,all of them were divided into 4 subgenus and regarded as 20valid names[1].展开更多
文摘运用PCR产物直接测序的方法对海南省文昌市90只文昌鸡样品的线粒体DNA控制区(mtDNA D-loop)第Ⅰ高变区序列进行了分析。用CLUSTAL X 1.81软件进行序列比较,其中38只个体共检测到27个核苷酸多态位点,全部为转换无颠换,序列突变率为5.29%。用DnaSP软件估计出序列存在16个单倍型,单倍型多样度(Hd)为0.935,核苷酸多样度(π)为0.01479,平均核苷酸差异数(k)为7.541。运用Mega4.0软件计算出不同个体间的遗传距离,并据此构建了NJ系统树。文昌鸡样品在NJ无根树上聚为4大分支。研究结果表明,文昌鸡群体内个体序列变异程度较大,遗传多样性丰富,揭示文昌鸡在遗传组成上具有4个母系来源。
基金Yunnan Provincial Science and Technology Program(2014FB178)National Key Technology R&D Program of China(2008BAC39B03)
文摘The genus Simocephalus Schoedler 1858,a large,nonpelagic daphniidae,are abundant in the littoral zone,expecially with submerged aquatic vegetation in freshwater bodies all over the world.More than 63 specific and subspecific names have been proposed.But in the recent research,all of them were divided into 4 subgenus and regarded as 20valid names[1].
