A micro-video imaging system on-line coupled with UV detection and capillary electrophoresis has been set up and used for the investigation of human red blood cells（hRBC）. Both free and aggregated cells were observe...A micro-video imaging system on-line coupled with UV detection and capillary electrophoresis has been set up and used for the investigation of human red blood cells（hRBC）. Both free and aggregated cells were observed via the imaging and the broad peak overlain by bar-like peaks measured from UV detection was shown responsible for the free migrated and aggregated cells, respectively. More importantly, fast measurement of cell mobility was achieved within one second using the image acquired and the calculated mobility data agreed with that of UV, giving a deviation of less than 7%. Further more, this micro-video system allows us to vividly observe the adsorption-desorption process. In a fused-silica capillary, about 10% of the human red blood cells were turned out to adsorb on the tubing surface, they left the surface after 0.04 s to 3.12 s, giving an average retarding time of less than 1 s. This causes a loss of migration mobility of 5×10-6 cm2·V-1·s-1. It was thought that this system be applicable to the study of adsorption of other types of molecules with some modification.展开更多
A method for the fast and accurate measurement of diffusion coefficient of minute proteins was developed. A protein sample plug was introduced into the capillary and forced to flow through a capillary coated dynamical...A method for the fast and accurate measurement of diffusion coefficient of minute proteins was developed. A protein sample plug was introduced into the capillary and forced to flow through a capillary coated dynamically by poly(diallyldimethylammonium chloride). Its concentration profile was then monitored by the UV absorption detector mounted on Beckman MDQ capillary electrophoresis system. Accurate diffusion coefficient was obtained. The key conditions for the accurate measurement include: (1) systematic peak should be developed in a laminar flow; (2) radius diffusion is negligible compared with the longitudinal diffusion; (3) the capillary is well thermostated and (4) the solute should not be overloaded. In common case, the deviation of the measurement was kept within 4%.展开更多
A method of color surface plasmon resonance imaging was developed and true color SPR images of solvents and BSA microdot arrays were recorded by using our laboratory-built device.The color signals,which were observed ...A method of color surface plasmon resonance imaging was developed and true color SPR images of solvents and BSA microdot arrays were recorded by using our laboratory-built device.The color signals,which were observed to be complementary to the resonance absorption of the incident light,depended on the feature of samples and the incident angle as expected.This new method looks to be a potential color analytical tool for high throughput detection of biochips.展开更多
文摘A micro-video imaging system on-line coupled with UV detection and capillary electrophoresis has been set up and used for the investigation of human red blood cells（hRBC）. Both free and aggregated cells were observed via the imaging and the broad peak overlain by bar-like peaks measured from UV detection was shown responsible for the free migrated and aggregated cells, respectively. More importantly, fast measurement of cell mobility was achieved within one second using the image acquired and the calculated mobility data agreed with that of UV, giving a deviation of less than 7%. Further more, this micro-video system allows us to vividly observe the adsorption-desorption process. In a fused-silica capillary, about 10% of the human red blood cells were turned out to adsorb on the tubing surface, they left the surface after 0.04 s to 3.12 s, giving an average retarding time of less than 1 s. This causes a loss of migration mobility of 5×10-6 cm2·V-1·s-1. It was thought that this system be applicable to the study of adsorption of other types of molecules with some modification.
文摘A method for the fast and accurate measurement of diffusion coefficient of minute proteins was developed. A protein sample plug was introduced into the capillary and forced to flow through a capillary coated dynamically by poly(diallyldimethylammonium chloride). Its concentration profile was then monitored by the UV absorption detector mounted on Beckman MDQ capillary electrophoresis system. Accurate diffusion coefficient was obtained. The key conditions for the accurate measurement include: (1) systematic peak should be developed in a laminar flow; (2) radius diffusion is negligible compared with the longitudinal diffusion; (3) the capillary is well thermostated and (4) the solute should not be overloaded. In common case, the deviation of the measurement was kept within 4%.
文摘A method of color surface plasmon resonance imaging was developed and true color SPR images of solvents and BSA microdot arrays were recorded by using our laboratory-built device.The color signals,which were observed to be complementary to the resonance absorption of the incident light,depended on the feature of samples and the incident angle as expected.This new method looks to be a potential color analytical tool for high throughput detection of biochips.
