目的研究CYP3A5基因多态性对肾移植受者他克莫司(FK506)血药浓度/剂量比(blood drug concentration/dose,C/D),术后急性排斥反应(AR)及不良反应发生率的影响。方法采用聚合酶链反应-限制性片段长度多态性技术检测肾移植受者CYP3A5基因型...目的研究CYP3A5基因多态性对肾移植受者他克莫司(FK506)血药浓度/剂量比(blood drug concentration/dose,C/D),术后急性排斥反应(AR)及不良反应发生率的影响。方法采用聚合酶链反应-限制性片段长度多态性技术检测肾移植受者CYP3A5基因型,并以直接测序法进行验证。比较不同基因型患者之间的FK506C/D值,AR、不良反应的发生率。结果肾移植术后3个月内,*3/*3基因型患者FK506的C/D值为149±59,明显高于*1/*1基因型和*1/*3基因型患者的78±24和90±42(均为P<0.05);移植术后3个月内*1/*1基因型患者的AR发生率与*1/*3基因型和*3/*3基因型患者的AR发生率比较差异无统计学意义。与*1/*1基因型和*1/*3基因型患者比较,*3/*3基因型患者术后肝功能异常、药物肾毒性不良反应的发生率较高(均为P<0.05)。结论 CYP3A5基因多态性对服用FK506肾移植患者的C/D值、肝功能异常、药物肾毒性有一定影响。CYP3A5*3/*3基因型患者的FK506的C/D值、肝功能异常和药物肾毒性不良反应的发生率较高。展开更多
It is difficult to determine estrogens directly by electrochemical methods because estradiol, estrone and estriol are hydrophobic and electroinactive. Thus, they are only determined by the indirect electrochemical tec...It is difficult to determine estrogens directly by electrochemical methods because estradiol, estrone and estriol are hydrophobic and electroinactive. Thus, they are only determined by the indirect electrochemical techniques. In this paper, based on the voltammetric behavior of estrogens(estradiol, estrone and estriol) on the Nafion\|modified glassy carbon electrode in the presence of CTAB, a novel direct electroanalytical method for the determination of the total amounts of estradiol, estrone and estriol was developed. In pH=8.00 phosphate buffer and in the presence of 6×10\+\{-5\} mol/L CTAB, estradiol, estrone and estriol have very sensitive oxidation peaks at 0.38 V(\%vs\%. SCE) at Nafion\|modified glassy carbon electrode after being accumulated for 3 min at -0.50 V. This peak can be used to determine trace level of estradiol, estrone and estriol. It was found that estradol, estrone and estriol have the similar electrochemical responses on the Nafion\|modified electrode in the presence of other long\|chain cationic surfactants, such as CPB, STAB, CATAB, LTAB. It is observed that the enhancement effect is highest in the presence of CTAB, and the peak current is CTAB concentration\|dependent. However, in the presence of long\|chain anionic surfactants, such as SDBS, and long\|chain nonionic surfactants, such as Triton X\|100, estradiol, estrone and estriol have no voltammetric responses on the Nafion\|modified glassy carbon electrode. The mechanism for the oxidation of estradiol in this system has been discussed, and the reactant site for the oxidation of estradiol has been verified to be the hyrdoxyl group in A\|ring.展开更多
文摘目的研究CYP3A5基因多态性对肾移植受者他克莫司(FK506)血药浓度/剂量比(blood drug concentration/dose,C/D),术后急性排斥反应(AR)及不良反应发生率的影响。方法采用聚合酶链反应-限制性片段长度多态性技术检测肾移植受者CYP3A5基因型,并以直接测序法进行验证。比较不同基因型患者之间的FK506C/D值,AR、不良反应的发生率。结果肾移植术后3个月内,*3/*3基因型患者FK506的C/D值为149±59,明显高于*1/*1基因型和*1/*3基因型患者的78±24和90±42(均为P<0.05);移植术后3个月内*1/*1基因型患者的AR发生率与*1/*3基因型和*3/*3基因型患者的AR发生率比较差异无统计学意义。与*1/*1基因型和*1/*3基因型患者比较,*3/*3基因型患者术后肝功能异常、药物肾毒性不良反应的发生率较高(均为P<0.05)。结论 CYP3A5基因多态性对服用FK506肾移植患者的C/D值、肝功能异常、药物肾毒性有一定影响。CYP3A5*3/*3基因型患者的FK506的C/D值、肝功能异常和药物肾毒性不良反应的发生率较高。
文摘It is difficult to determine estrogens directly by electrochemical methods because estradiol, estrone and estriol are hydrophobic and electroinactive. Thus, they are only determined by the indirect electrochemical techniques. In this paper, based on the voltammetric behavior of estrogens(estradiol, estrone and estriol) on the Nafion\|modified glassy carbon electrode in the presence of CTAB, a novel direct electroanalytical method for the determination of the total amounts of estradiol, estrone and estriol was developed. In pH=8.00 phosphate buffer and in the presence of 6×10\+\{-5\} mol/L CTAB, estradiol, estrone and estriol have very sensitive oxidation peaks at 0.38 V(\%vs\%. SCE) at Nafion\|modified glassy carbon electrode after being accumulated for 3 min at -0.50 V. This peak can be used to determine trace level of estradiol, estrone and estriol. It was found that estradol, estrone and estriol have the similar electrochemical responses on the Nafion\|modified electrode in the presence of other long\|chain cationic surfactants, such as CPB, STAB, CATAB, LTAB. It is observed that the enhancement effect is highest in the presence of CTAB, and the peak current is CTAB concentration\|dependent. However, in the presence of long\|chain anionic surfactants, such as SDBS, and long\|chain nonionic surfactants, such as Triton X\|100, estradiol, estrone and estriol have no voltammetric responses on the Nafion\|modified glassy carbon electrode. The mechanism for the oxidation of estradiol in this system has been discussed, and the reactant site for the oxidation of estradiol has been verified to be the hyrdoxyl group in A\|ring.
