The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activ...The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1\|cell stage fertilized eggs were semi\|quantitatively assayed by Western blotting and RT\|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1\|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1\|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1\|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1\|cell stage fertilized eggs.After microinjecting LY294002 the development from 1\|cell stage to 2\|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1\|cell to 2\|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.展开更多
Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse tes...Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.展开更多
文摘The role of protein kinase B (PKB) in the regulation of early development of fertilized eggs in mouse and its biological function on the development of mouse 1\|cell stage fertilized eggs were studied.The kinase activity of PKB was detected,and mRNA and protein level of PKB both in MⅡ oocytes and 1\|cell stage fertilized eggs were semi\|quantitatively assayed by Western blotting and RT\|PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1\|cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1\|cell stage fertilized eggs.The results showed that the activity of PKB increased obviously after the fertilization,the mRNA and protein level of PKB also increased obviously in 1\|cell stage fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The protein level was lower than that in the 1\|cell stage fertilized eggs.After microinjecting LY294002 the development from 1\|cell stage to 2\|cell stage was inhibited obviously.All the results demonstrate that PKB exists in the fertilized eggs,and the activity,expression and transcription level increase after fertilization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1\|cell to 2\|cell stage,but the mechanism is still unknown.These experiments are helpful for the further study of PKB on the regulation of early development of fertilized eggs.
文摘Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.
