According to the published nucleotide sequences of the VP1 gene of foot-and-mouth disease virus serotype Asia 1 isolates, a pair of primers were designed and synthesized to clone the VP1 gene of YNBS/58 strain. PCR pr...According to the published nucleotide sequences of the VP1 gene of foot-and-mouth disease virus serotype Asia 1 isolates, a pair of primers were designed and synthesized to clone the VP1 gene of YNBS/58 strain. PCR product was cloned into pProexHTb vector, and E.coli BL21 was transformed by the recombinant plasmid pProex-VP1 for sequencing and expression .The expressed product was identified by SDS-PAGE and Western blot, and purified by Ni-NTA His.Bind resins. The results showed that the nucleotide sequence identity of VP1 gene between YNBS/58 and India93, India 97,India99, Iseral and YNAs1.1 strains is from 80.3% to 97.5% and amino acid identity is from 85.8% to 96.5%, the recombinant VP1 protein was significant at 34 ku by SDS-PAGE and Western blot, which accounted for 30% of total protein in E.coli lysates,and the recombinant protein was purified successfully.展开更多
口蹄疫（Foot and mouth disease，FMD）是由FMD病毒（FMDV）引起的偶蹄动物的烈性传染病，患病动物的口、舌、唇、蹄、乳房等部位发生水泡，水泡破溃后形成烂斑。由于该病传染性强，造成的经济损失严重，世界动物卫生组织（OIE）将该...口蹄疫（Foot and mouth disease，FMD）是由FMD病毒（FMDV）引起的偶蹄动物的烈性传染病，患病动物的口、舌、唇、蹄、乳房等部位发生水泡，水泡破溃后形成烂斑。由于该病传染性强，造成的经济损失严重，世界动物卫生组织（OIE）将该病列为实时通报的传染病之一。展开更多
文摘According to the published nucleotide sequences of the VP1 gene of foot-and-mouth disease virus serotype Asia 1 isolates, a pair of primers were designed and synthesized to clone the VP1 gene of YNBS/58 strain. PCR product was cloned into pProexHTb vector, and E.coli BL21 was transformed by the recombinant plasmid pProex-VP1 for sequencing and expression .The expressed product was identified by SDS-PAGE and Western blot, and purified by Ni-NTA His.Bind resins. The results showed that the nucleotide sequence identity of VP1 gene between YNBS/58 and India93, India 97,India99, Iseral and YNAs1.1 strains is from 80.3% to 97.5% and amino acid identity is from 85.8% to 96.5%, the recombinant VP1 protein was significant at 34 ku by SDS-PAGE and Western blot, which accounted for 30% of total protein in E.coli lysates,and the recombinant protein was purified successfully.
