Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and 1H NMR.Linoleoyl insulin was prepared by chemical modifi...Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and 1H NMR.Linoleoyl insulin was prepared by chemical modification of insulin with linoleoyl benzotriazole without using any specific protecting group.MALDI-TOF-MS analysis showed that the modification of insulin was successful and the monoacylated insulin was the major product.A little diacylated insulin was observed and no triacylated insulin was detected.展开更多
文摘Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and 1H NMR.Linoleoyl insulin was prepared by chemical modification of insulin with linoleoyl benzotriazole without using any specific protecting group.MALDI-TOF-MS analysis showed that the modification of insulin was successful and the monoacylated insulin was the major product.A little diacylated insulin was observed and no triacylated insulin was detected.
