Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and 1H NMR.Linoleoyl insulin was prepared by chemical modifi...Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and 1H NMR.Linoleoyl insulin was prepared by chemical modification of insulin with linoleoyl benzotriazole without using any specific protecting group.MALDI-TOF-MS analysis showed that the modification of insulin was successful and the monoacylated insulin was the major product.A little diacylated insulin was observed and no triacylated insulin was detected.展开更多
An enzymatic kinetic spectrophotometric method was applied to determine zinc based on the inhibition of zinc for the catalytic reaction of pyruvate and nicotinamide adenine dinucleotide reduced form(NADH) by lactate d...An enzymatic kinetic spectrophotometric method was applied to determine zinc based on the inhibition of zinc for the catalytic reaction of pyruvate and nicotinamide adenine dinucleotide reduced form(NADH) by lactate dehydrogenase.The rate of the absorbance change of NADH was determined for calculating the velocity of the enzymatic reaction and calibration curve of zinc was prepared.The effects of the pH value and the coexisted ions in the solution were discussed.This method has advatages of rapidity,sensitivity and less interference.展开更多
文摘Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and 1H NMR.Linoleoyl insulin was prepared by chemical modification of insulin with linoleoyl benzotriazole without using any specific protecting group.MALDI-TOF-MS analysis showed that the modification of insulin was successful and the monoacylated insulin was the major product.A little diacylated insulin was observed and no triacylated insulin was detected.
文摘An enzymatic kinetic spectrophotometric method was applied to determine zinc based on the inhibition of zinc for the catalytic reaction of pyruvate and nicotinamide adenine dinucleotide reduced form(NADH) by lactate dehydrogenase.The rate of the absorbance change of NADH was determined for calculating the velocity of the enzymatic reaction and calibration curve of zinc was prepared.The effects of the pH value and the coexisted ions in the solution were discussed.This method has advatages of rapidity,sensitivity and less interference.
