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香茶子叶片总酚、总黄酮提取及抗氧化性研究 被引量:16
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作者 邹维娜 王微 +2 位作者 徐启江 杜伟伟 李灵玉 《食品工业科技》 CAS CSCD 北大核心 2017年第2期266-272,共7页
目的:获得微波辅助提取香茶藨子叶片中总酚及总黄酮的最佳工艺,以及香茶藨子叶片中总酚及总黄酮的体外抗氧化活性。方法:在单因素的基础上,采用响应面法对影响总酚和总黄酮提取量的主要因素进行分析,探讨微波辅助提取香茶藨子叶片中总... 目的:获得微波辅助提取香茶藨子叶片中总酚及总黄酮的最佳工艺,以及香茶藨子叶片中总酚及总黄酮的体外抗氧化活性。方法:在单因素的基础上,采用响应面法对影响总酚和总黄酮提取量的主要因素进行分析,探讨微波辅助提取香茶藨子叶片中总酚及总黄酮的最优工艺,并与超声提取、索氏提取进行比较。采用DPPH和FRAP法对香茶藨子叶片中总酚及总黄酮的自由基清除能力和总抗氧化活性进行评价。结果:最佳的提取工艺条件为:提取温度58℃,液固比39∶1 m L/g,乙醇浓度57%,提取时间15 min和微波功率600 W,此条件下总酚及总黄酮的提取量分别为99.89、38.16 mg/g。微波辅助提取得到的香茶藨子叶片提取物对DPPH自由基有很好的清除作用,IC50值为0.0445 mg/m L,此外其还有较高的总抗氧化能力,FRAP值为4.832 mmol/g。结论:优选的总酚、总黄酮提取工艺稳定可行,香茶藨子叶片中总酚及总黄酮有较好的抗氧化活性,为其研究开发提供一定的科学依据。 展开更多
关键词 香茶藨子 总酚 总黄酮 响应面法 微波辅助提取 抗氧化
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Optimization of Solvent-Free Microwave Extraction of Essential Oil from the Fruits of Schisandra chinensis and Its DPPH Radical Scavenging Activity
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作者 CHEN Xiao-qiang ZHANG Ying +2 位作者 ZU Yuan-gang YU Xue-ying LI Jia-lei 《食品科学》 EI CAS CSCD 北大核心 2011年第14期85-89,共5页
This study was undertaken to optimize the solvent-free microwave extraction conditions and DPPH radicalscavenging activity of essential oil from Schisandra chinesis fruits.The uniform design method was employed for pr... This study was undertaken to optimize the solvent-free microwave extraction conditions and DPPH radicalscavenging activity of essential oil from Schisandra chinesis fruits.The uniform design method was employed for process optimization.The optimal extraction conditions were determined as follows:extraction time,50 rain;microwave power,800 W;and amount of water addition for pretreatment,40%.Under these conditions,the extraction yield of essential oil was 0.92%.A total of 35 compounds were identified by GC-MS in the obtained essential oil with a total content of 91.06%,mostly consisting of ylangene(34.81%),β-himachalene(10.74%)andα-bergamotene(9.22%).The ICs0 value of the essential oil against DPPH free radicals was determined as 3.01 mg/mL.In conclusion,solvent-free microwave extraction is a feasible method for essential oil extraction from Schisandra chinensis fruits. 展开更多
关键词 Schisandra chinesis essential oil solvent-free microwave extraction chemical composition DPPH radical scavenging activity
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