采用用计算化学方法研究金丝桃素分子结构特征,并用分子动力学方法研究其与HIV蛋白酶的相互作用,探讨其可能的抗HIV病毒作用机理.结果表明,金丝桃素分子结构具有刚性特征,与HIV蛋白酶在酶的催化活性位点与ASP-A25 and ASP-B25以氢键作...采用用计算化学方法研究金丝桃素分子结构特征,并用分子动力学方法研究其与HIV蛋白酶的相互作用,探讨其可能的抗HIV病毒作用机理.结果表明,金丝桃素分子结构具有刚性特征,与HIV蛋白酶在酶的催化活性位点与ASP-A25 and ASP-B25以氢键作用相结合.展开更多
The interaction between β-K 4H 3 [SiW 9O 37(CpTi) 3]·11H 2O and DNA was investigated by agarose gel electrophoresis. The results suggest that β-K 4H 3[SiW 9O 37(CpTi) 3]·11H 2O can degrade the genomic DNA....The interaction between β-K 4H 3 [SiW 9O 37(CpTi) 3]·11H 2O and DNA was investigated by agarose gel electrophoresis. The results suggest that β-K 4H 3[SiW 9O 37(CpTi) 3]·11H 2O can degrade the genomic DNA. The treatment of plasmid DNA with β-K 4H 3 [SiW 9O 37(CpTi) 3]·11H 2O resulted in a fast moving band in front of the three normal conformations(super coiled DNA, linear DNA and relaxed circular DNA) in agarose gel electrophoresis . These results indicate that this chemical material may have the ability to specifically cleavage DNA or to alter conformation of DNA molecules.展开更多
The kinetic analysis of the interaction between tumor necrosis factor(TNF) and its monoclonal antibody was performed by surface plasmon resonance(SPR) technique. The monoclonal antibody was immobilized to the surface ...The kinetic analysis of the interaction between tumor necrosis factor(TNF) and its monoclonal antibody was performed by surface plasmon resonance(SPR) technique. The monoclonal antibody was immobilized to the surface of CM5 sensor chip by amine coupling. TNF at different concentrations was injected across the mAb immobilized surface. The interaction was recorded in real time and could be seen on the sensorgram. One cycle, including association, dissociation and regeneration, lasted no more than 15 min . The interaction results was evaluated using 1∶1 Langmuir binding model. The kinetic rate constants were calculated to be: k a=1.68×10 3 L·mol -1 ·s -1 , k d=1.73×10 -4 s -1 , and the affinity constants K A=9.7×10 6 L·mol -1 , K D=1.03×10 -7 mol·L -1 . The χ \+2 was 3.47, which showed that the interaction is consistent with the 1∶1 model. We can see from the results that although there are two binding sites in one mAb molecule, TNF reacts with each site in an independent and noncooperative manner.展开更多
In this paper, the fixing and stretching effect of Ni(phen) 2+ 3 with different concentrations on DNA had been studied by Tapping mode AFM. Under an ambient condition, the high resolution DNA images were obtained, the...In this paper, the fixing and stretching effect of Ni(phen) 2+ 3 with different concentrations on DNA had been studied by Tapping mode AFM. Under an ambient condition, the high resolution DNA images were obtained, the average height, width and length of well spread DNA molecules were measured. The results showed that because of the variations of ionic concentration, the density and topography of DNA molecules on substrate had a great difference. The AFM and gel electrophoresis results also showed that, under our experimental condition, DNA molecules kept intact, Ni(phen) 2+ 3 did not catalyze the cleavage activity of EcoRI, therefore, Ni(phen) 2+ 3 would be used to make high resolution physical mapping of DNA by AFM.展开更多
文摘The interaction between β-K 4H 3 [SiW 9O 37(CpTi) 3]·11H 2O and DNA was investigated by agarose gel electrophoresis. The results suggest that β-K 4H 3[SiW 9O 37(CpTi) 3]·11H 2O can degrade the genomic DNA. The treatment of plasmid DNA with β-K 4H 3 [SiW 9O 37(CpTi) 3]·11H 2O resulted in a fast moving band in front of the three normal conformations(super coiled DNA, linear DNA and relaxed circular DNA) in agarose gel electrophoresis . These results indicate that this chemical material may have the ability to specifically cleavage DNA or to alter conformation of DNA molecules.
文摘The kinetic analysis of the interaction between tumor necrosis factor(TNF) and its monoclonal antibody was performed by surface plasmon resonance(SPR) technique. The monoclonal antibody was immobilized to the surface of CM5 sensor chip by amine coupling. TNF at different concentrations was injected across the mAb immobilized surface. The interaction was recorded in real time and could be seen on the sensorgram. One cycle, including association, dissociation and regeneration, lasted no more than 15 min . The interaction results was evaluated using 1∶1 Langmuir binding model. The kinetic rate constants were calculated to be: k a=1.68×10 3 L·mol -1 ·s -1 , k d=1.73×10 -4 s -1 , and the affinity constants K A=9.7×10 6 L·mol -1 , K D=1.03×10 -7 mol·L -1 . The χ \+2 was 3.47, which showed that the interaction is consistent with the 1∶1 model. We can see from the results that although there are two binding sites in one mAb molecule, TNF reacts with each site in an independent and noncooperative manner.
文摘In this paper, the fixing and stretching effect of Ni(phen) 2+ 3 with different concentrations on DNA had been studied by Tapping mode AFM. Under an ambient condition, the high resolution DNA images were obtained, the average height, width and length of well spread DNA molecules were measured. The results showed that because of the variations of ionic concentration, the density and topography of DNA molecules on substrate had a great difference. The AFM and gel electrophoresis results also showed that, under our experimental condition, DNA molecules kept intact, Ni(phen) 2+ 3 did not catalyze the cleavage activity of EcoRI, therefore, Ni(phen) 2+ 3 would be used to make high resolution physical mapping of DNA by AFM.
