A novel monolithic stationary phase with long alkyl chain ligands was introduced and evaluated in pressurized capillary electrochromatography(p-CEC) by using a mixture of neutral and charged compounds. The capillary m...A novel monolithic stationary phase with long alkyl chain ligands was introduced and evaluated in pressurized capillary electrochromatography(p-CEC) by using a mixture of neutral and charged compounds. The capillary monolithic-column was prepared by in situ copolymerization of ethylene dimethacrylate,alkyl alcohol and 2-acrylamido-2-methyl-1-propanesulfonic acid in a quadruple porogenic solvent consisting of 1,4-butanediol/cyclohexanol/docecanol/water. Effects of experimental parameters in p-CEC,such as pressure and electrical field strength,on the separation were investigated. The baseline separation of the components in the mixture was achieved. The monolithic column can be used as an alternative to the conventional capillary column such as the reversed phase ODS.展开更多
以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋...以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋白(BSA)和溶菌酶(Lys)保留行为的影响。实验结果表明,该染料亲和填料具有良好的色谱性能。利用前沿色谱法测定了染料与溶菌酶之间的表观解离常数为5.26×10-5mol/L。使用该填料成功地从鸡蛋清和小牛血清中分别分离纯化了Lys和BSA,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示Lys和BSA的纯度分别为95%和92%。展开更多
文摘A novel monolithic stationary phase with long alkyl chain ligands was introduced and evaluated in pressurized capillary electrochromatography(p-CEC) by using a mixture of neutral and charged compounds. The capillary monolithic-column was prepared by in situ copolymerization of ethylene dimethacrylate,alkyl alcohol and 2-acrylamido-2-methyl-1-propanesulfonic acid in a quadruple porogenic solvent consisting of 1,4-butanediol/cyclohexanol/docecanol/water. Effects of experimental parameters in p-CEC,such as pressure and electrical field strength,on the separation were investigated. The baseline separation of the components in the mixture was achieved. The monolithic column can be used as an alternative to the conventional capillary column such as the reversed phase ODS.
文摘以3.0μm无孔单分散亲水性交联聚甲基丙烯酸环氧丙酯树脂为基质,与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制备出一种固定化染料聚合物高效亲和色谱填料。考察了应用该填料时流动相中的盐离子浓度、有机溶剂及流速等对牛血清白蛋白(BSA)和溶菌酶(Lys)保留行为的影响。实验结果表明,该染料亲和填料具有良好的色谱性能。利用前沿色谱法测定了染料与溶菌酶之间的表观解离常数为5.26×10-5mol/L。使用该填料成功地从鸡蛋清和小牛血清中分别分离纯化了Lys和BSA,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示Lys和BSA的纯度分别为95%和92%。
