目的 探讨眼组织银染核仁组成区 (Ag NOR)染色的效果和意义。方法 对部分正常眼组织、激光屈光性角膜切削术后角膜组织及小梁切除术滤过泡区组织进行 Ag NOR染色 ,光镜下观察组织结构和银染颗粒。结果 Ag NOR染色可清晰地显示出眼...目的 探讨眼组织银染核仁组成区 (Ag NOR)染色的效果和意义。方法 对部分正常眼组织、激光屈光性角膜切削术后角膜组织及小梁切除术滤过泡区组织进行 Ag NOR染色 ,光镜下观察组织结构和银染颗粒。结果 Ag NOR染色可清晰地显示出眼组织结构和细胞核中银染颗粒。增殖旺盛的细胞核中银染颗粒数增多。结论 Ag展开更多
Objective To evaluate the level of apoptosis in the corneal epithelium after photorefractive keratectomy(PRK).Methods Six rabbits were anesthetized systemically with sodium pentobarbital.PRK was then performed and the...Objective To evaluate the level of apoptosis in the corneal epithelium after photorefractive keratectomy(PRK).Methods Six rabbits were anesthetized systemically with sodium pentobarbital.PRK was then performed and the central 5.6mm area of the cornea was ablated to a depth of 156μm(-8.0D).Rabbits were killed 1 month and 3 months after the performance respectivelly,and the cornea were prepared for frozen tissue sections.The apoptosis of the corneal epithelium was evaluated by TUNEL method.Results The normal corneal epithelium exhibited a limited level of apoptosis,primarily in the superficial epithelium.The entire epithelial layer was found to be apoptotic 1 month after PRK.The apoptosis of the corneal epithelium 3 months after PRK was the same as the normal corneal epithelium.Conclusion The apoptosis of corneal epithelium exist in wound healing process after PRK.The findings suggest that apoptosis plays an important role in the corneal wound healing process.展开更多
文摘目的 探讨眼组织银染核仁组成区 (Ag NOR)染色的效果和意义。方法 对部分正常眼组织、激光屈光性角膜切削术后角膜组织及小梁切除术滤过泡区组织进行 Ag NOR染色 ,光镜下观察组织结构和银染颗粒。结果 Ag NOR染色可清晰地显示出眼组织结构和细胞核中银染颗粒。增殖旺盛的细胞核中银染颗粒数增多。结论 Ag
文摘Objective To evaluate the level of apoptosis in the corneal epithelium after photorefractive keratectomy(PRK).Methods Six rabbits were anesthetized systemically with sodium pentobarbital.PRK was then performed and the central 5.6mm area of the cornea was ablated to a depth of 156μm(-8.0D).Rabbits were killed 1 month and 3 months after the performance respectivelly,and the cornea were prepared for frozen tissue sections.The apoptosis of the corneal epithelium was evaluated by TUNEL method.Results The normal corneal epithelium exhibited a limited level of apoptosis,primarily in the superficial epithelium.The entire epithelial layer was found to be apoptotic 1 month after PRK.The apoptosis of the corneal epithelium 3 months after PRK was the same as the normal corneal epithelium.Conclusion The apoptosis of corneal epithelium exist in wound healing process after PRK.The findings suggest that apoptosis plays an important role in the corneal wound healing process.
