Field trials with a set of 108 doubled haploid lines(DHs) derived from a cross between the Chinese winter wheat cvs.CA9613 and H1488 were run at Beijing(China).Phenotypic data were recorded for major agronomic yield t...Field trials with a set of 108 doubled haploid lines(DHs) derived from a cross between the Chinese winter wheat cvs.CA9613 and H1488 were run at Beijing(China).Phenotypic data were recorded for major agronomic yield traits,i.e.grain weight per ear,grain number per ear and thousand grain weight(Tgw) in two field trials at Beijing.Based on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci(QTL) was carried out for yield and yield parameters using the composite interval mapping(CIM) approach.A total of 14 QTL were detected for these traits across two environments.Four of these QTL located on chromosomes 1A and 2B,respectively,exhibited pleiotropic effects.Loci showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate("pyramide") favorable alleles at different loci.展开更多
mtDNAs of T type wheat cytoplasmic male sterile lines Ning Drawf 14(ND14) and their maintainers Ning Drawf 13 (ND13) were isolated and digested completely with restriction endonucleases EcoRI, PstI, EcoRV, BamHI. The ...mtDNAs of T type wheat cytoplasmic male sterile lines Ning Drawf 14(ND14) and their maintainers Ning Drawf 13 (ND13) were isolated and digested completely with restriction endonucleases EcoRI, PstI, EcoRV, BamHI. The results revealed that the molecular structure of mtDNAs from ND14 and ND13 cytoplasms were significantly deviated. The mitochondrial genomic difference between CMS line and maintainers were uncovered by southern hybridization with probes of 18S+5S rRNA、atpA genes from wheat and pea mitochondria, respectively. Due to the complexity of mtDNA and no proof of protein difference, it has not yet been demonstrated whether mtDNA difference of Normal and Male Sterile Cytoplasm of wheat is associated with CMS.展开更多
Yield characters, maturity and grain protein content of somaclones derived both from immature embryo of cultivar 77(2)-Spring and single-cell culture of cultivar NE7742 in vitro were studied and the wide variation was...Yield characters, maturity and grain protein content of somaclones derived both from immature embryo of cultivar 77(2)-Spring and single-cell culture of cultivar NE7742 in vitro were studied and the wide variation was found. Somaclones with maturity 8 days earlier than or the same as CK NE 7742 (high yield, early maturity and high quality), combining with high quality (grain protein content 15.5% - 18%) and high yield (the same as 7724 or higher) have been found and selected and now multiplied for 8 generations. The results of cultivar comparison trial in 1995 showed that several somaclones (the yields were significantly higher than CK DN120) could be used directly in wheat production. The studies confirmed that somaclonal variation is one of the effective ways for early maturity, high-yielding and high-quality improvement in wheat.展开更多
文摘Field trials with a set of 108 doubled haploid lines(DHs) derived from a cross between the Chinese winter wheat cvs.CA9613 and H1488 were run at Beijing(China).Phenotypic data were recorded for major agronomic yield traits,i.e.grain weight per ear,grain number per ear and thousand grain weight(Tgw) in two field trials at Beijing.Based on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci(QTL) was carried out for yield and yield parameters using the composite interval mapping(CIM) approach.A total of 14 QTL were detected for these traits across two environments.Four of these QTL located on chromosomes 1A and 2B,respectively,exhibited pleiotropic effects.Loci showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate("pyramide") favorable alleles at different loci.
文摘mtDNAs of T type wheat cytoplasmic male sterile lines Ning Drawf 14(ND14) and their maintainers Ning Drawf 13 (ND13) were isolated and digested completely with restriction endonucleases EcoRI, PstI, EcoRV, BamHI. The results revealed that the molecular structure of mtDNAs from ND14 and ND13 cytoplasms were significantly deviated. The mitochondrial genomic difference between CMS line and maintainers were uncovered by southern hybridization with probes of 18S+5S rRNA、atpA genes from wheat and pea mitochondria, respectively. Due to the complexity of mtDNA and no proof of protein difference, it has not yet been demonstrated whether mtDNA difference of Normal and Male Sterile Cytoplasm of wheat is associated with CMS.
文摘Yield characters, maturity and grain protein content of somaclones derived both from immature embryo of cultivar 77(2)-Spring and single-cell culture of cultivar NE7742 in vitro were studied and the wide variation was found. Somaclones with maturity 8 days earlier than or the same as CK NE 7742 (high yield, early maturity and high quality), combining with high quality (grain protein content 15.5% - 18%) and high yield (the same as 7724 or higher) have been found and selected and now multiplied for 8 generations. The results of cultivar comparison trial in 1995 showed that several somaclones (the yields were significantly higher than CK DN120) could be used directly in wheat production. The studies confirmed that somaclonal variation is one of the effective ways for early maturity, high-yielding and high-quality improvement in wheat.
