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甲醛固定对Live/Dead BacLight Bacterial Viability Kit死活细菌染液荧光显微计数海洋细菌的影响 被引量:2
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作者 张瑜斌 崔焱芸 +1 位作者 郑运 孙省利 《生态科学》 CSCD 北大核心 2013年第5期636-641,共6页
利用Live/Dead BacLight Bacterial Viability Kit死活细菌染液对采自湛江东海大堤海水、沉积物细菌和大型海藻拟刚毛藻(Cladophoropsis zollingeri)内生细菌数量进行了甲醛固定处理前后的荧光显微计数对比分析。结果表明,新鲜样品(不... 利用Live/Dead BacLight Bacterial Viability Kit死活细菌染液对采自湛江东海大堤海水、沉积物细菌和大型海藻拟刚毛藻(Cladophoropsis zollingeri)内生细菌数量进行了甲醛固定处理前后的荧光显微计数对比分析。结果表明,新鲜样品(不加甲醛固定)、甲醛刚固定样品、甲醛固定1周样品和甲醛固定2周样品中海洋细菌数量差异不显著(p>0.05)。甲醛固定对Live/Dead BacLight Bacterial Viability Kit死活细菌染液荧光显微计数海洋细菌数量无显著影响,固定后的样品可在2周内完成计数。 展开更多
关键词 甲醛固定 LIVE DEAD BacLight Bacterial viability Kit染液 细菌荧光显微计数
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Effects of primary PCI and facilitated PCI on myocardial viability and ventricular systolic synchrony in acute myocardial infarction patients
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作者 谷新顺 傅向华 马宁 《介入放射学杂志》 CSCD 2003年第S1期150-,共1页
Objective To evaluate short time effects of primary percutaneous coronary intervention (pPCI) and rtPA thrombolysis+PCI (rtPA+PCI) on myocardial viability and ventricular systolic synchrony in AMI patients.Methods Eig... Objective To evaluate short time effects of primary percutaneous coronary intervention (pPCI) and rtPA thrombolysis+PCI (rtPA+PCI) on myocardial viability and ventricular systolic synchrony in AMI patients.Methods Eighty seven patients with first AMI were divided into two groups: group A ( n =42), pPCI group, the patients underwent PCI within 6h after onset of AMI; group B ( n =45), rtPA+PCI group, the patients underwent PCI after thrombolysis within 6h after onset of AMI; Myocardial viability was measured by 99m Tc MIBI SPECT. While, the parameters of cardiac function LVEF and ventricular systolic synchrony LVPS were measured by 99m Tc gated cardiac blood pool image on the first and the fourth weekend. Results (1) The peak CK MB was significantly lower in group A than that in group B( P <0.01 ). (2) Myocardial infarction area (MIA) was decreased and radioactivity counts in MIA was significantly increased in group A and B on the 4th weekend compared with that on the first weekend ( P <0.01 ), but there were no significant difference between group A and group B. (3) LVEF, LVPS were no significant difference between group A and group B.Conclusions (1)pPCI in acute myocardial infartion can limit infarct area, maintain ventricular systolic synchrony and improve ventricular function; (2) but, in those hospitals that there were no any condition for PCI, they should transfer the patients to central hospital for PCI after thrombolysis at the first time. It is beneficial to improve myocardial viability and ventricular systolic synchrony of AMI patients in short time. 展开更多
关键词 PCI 石家庄 河北 Effects of primary PCI and facilitated PCI on myocardial viability and ventricular systolic synchrony in acute myocardial infarction patients 河北医科大学第二医院 in on of
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Assessment of Economic Viability of Mini-livestock Production in Delta State,Nigeria:Implication for Extension Delivery Services
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作者 Ebewore Solomon Okeoghene Achoja Felix Odemero 《Journal of Northeast Agricultural University(English Edition)》 CAS 2016年第1期74-82,共9页
The study examined the popularity, role and economic viability of mini-livestock keeping in Delta State, Nigeria. The specific objectives were to describe the socio-economic characteristics of mini-livestock farmers, ... The study examined the popularity, role and economic viability of mini-livestock keeping in Delta State, Nigeria. The specific objectives were to describe the socio-economic characteristics of mini-livestock farmers, identify the types of animals reared as mini-livestock in the area, ascertain the reasons for keeping mini-livestock by farmers, determine the benefit level of mini- livestock farmers, and identify the problems facing mini-livestock farmers in the study area. Using snowball sampling technique, 674 respondents were sampled with the aid of a structured questionnaire. Various descriptive and inferential statistics were used for data analyses. The results of the study indicated various types of mini-livestock reared in the study area, mainly snail farming or heliciculture (about 90%) and rearing of cane rat (84.27%); the most important reasons for keeping mini-livestock were income (99.6%), employment (99.26%) and food (94.96%). The result further indicated that protein consumption and improved income were the most important benefits to respondents. In spite of these benefits, the respondents faced some constraints, amongst which were lack of technical information (X=4.70), lacking of enough space for expansion (X=4.58), inadequate training (X=4.20), poor extension delivery services (X=4.20) and problems of feed availability (X=3.86). In spite of these constraints, the production of mini-livestock was found to be profitable in the study area. Based on the t'mdings, it was recommended, among others, that mini-livestock farmers in the study area should be trained on various aspects of the management of mini-livestock especially by organising workshops for them. 展开更多
关键词 mini-livestock earle rat guinea pig viability SNAIL heliciculture
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Viability of Two Freeze-dried Strains of Bifidobacterium Preparations at Various Temperatures during Prolonged Storage
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作者 DUPeng DULi-hui HUOGui-cheng 《Journal of Northeast Agricultural University(English Edition)》 CAS 2005年第1期48-52,共5页
Viability of bifidobacteria in freeze-dried probiotic products at various temperatures during prolonged storage was assessed. Bifidobacterium longum and Bifidobacterium infantis were freeze-dried. The freeze-dried pre... Viability of bifidobacteria in freeze-dried probiotic products at various temperatures during prolonged storage was assessed. Bifidobacterium longum and Bifidobacterium infantis were freeze-dried. The freeze-dried preparations were stored at -18 , 4 , and 20 ℃. Cell counts were enumerated using BS agar at 37 ℃ for 48 h under anaerobic conditions at 0, 45 and 120 days. Storage at 20 ℃ showed the greatest decline in the viability of bifidobacteria, whereas that at -18 ℃ showed the least decrease. 展开更多
关键词 BIFIDOBACTERIUM viability storage temperature FREEZE-DRYING
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小麦种子在不同贮存条件下保存10年后生活力的差异 被引量:3
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作者 张志娥 石思信 肖建平 《种子》 CSCD 北大核心 1993年第1期19-22,共4页
冬小麦农大139含水量为6.7%和11.8%。分别密封保存于室温和0~5℃冰箱。10年后,测定其发芽势,发芽率等指标,结果表明:①在室温条件下,含水量为11.8%的种子全部死亡,含水量为6.7%的种子有近50%的发芽率同保存前种子发芽率相比明显下降。... 冬小麦农大139含水量为6.7%和11.8%。分别密封保存于室温和0~5℃冰箱。10年后,测定其发芽势,发芽率等指标,结果表明:①在室温条件下,含水量为11.8%的种子全部死亡,含水量为6.7%的种子有近50%的发芽率同保存前种子发芽率相比明显下降。②在0~5℃冰箱保存的种子,两种含水量均达到80%左右的发芽率,同保存前相比没有明显差别。统计分析表明,贮存温度及贮存温度和种子含水量的互作对贮存种子活力的影响达到极显著水平。 展开更多
关键词 Seed viability Storage Temperature Water content.
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Generation of dynamically perfused functional vascular network system within hydrogel using3D bio-printing technology 被引量:1
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作者 Vivian K.Lee Seung-Schik Yoo +1 位作者 Peter Vincent Guohao Dai 《医用生物力学》 EI CAS CSCD 北大核心 2013年第S1期9-11,共3页
Introduction:3D bio-printing technology<sup>[1-3]</sup>(Figure 1)capable of dispensing live cells,soluble factors,and phase-changing hydrogel in a desired pattern,has great potential in creating 3D tissu... Introduction:3D bio-printing technology<sup>[1-3]</sup>(Figure 1)capable of dispensing live cells,soluble factors,and phase-changing hydrogel in a desired pattern,has great potential in creating 3D tissue.However,maintaining the viability of a thick tissue structure during tissue growth and maturation is challenging due to lack of adequate vascular perfusion.In a simple tissue model with 展开更多
关键词 HYDROGEL PRINTING PERFUSION dynamically challenging viability maintaining DESIRED thick capable
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Where are You Going,Philosophy, and What are Your Methods?
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作者 Gila Sher 《学术界》 CSSCI 北大核心 2020年第12期185-196,共12页
The viability of philosophy as a genuine field of knowledge has been challenged time and again.Some have challenged“traditional”philosophy,or what was considered“traditional philosophy”at a given time;others have ... The viability of philosophy as a genuine field of knowledge has been challenged time and again.Some have challenged“traditional”philosophy,or what was considered“traditional philosophy”at a given time;others have challenged philosophy in general.But there has been considerable progress in philosophical methodology in the 20th-and 21st-centuries.In this paper I raise challenges to some of the current misconceptions of analytic philosophy,and I propose constructive methodological solutions. 展开更多
关键词 viability of philosophy fundementalism Quinean holism foundational holism INTELLECT
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Analysis on Membrane Stability of Crown in Winter Wheat in Frigid Region in China
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作者 Wang Xiao-nan Xie Dong-wei +3 位作者 Fu Lian-shuang Sun Ying-lu Li Zhuo-fu Luan Fei-shi 《Journal of Northeast Agricultural University(English Edition)》 CAS 2013年第2期1-11,F0003,共12页
Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive ... Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water composition and content, and the changes in cell viability in the crowns, which contain the growing point, were studied during the period from cold acclimation to the deep freezing. The results showed that electrical conductivity which reflects the degree of plasma membrane damaged under low temperature was significantly correlated with the free water and the total water content. The malondialdehyde (MDA) content, which reflects the degree of membrane peroxidation, was very significantly correlated with superoxide dismutase (SOD), peroxidase, and ascorbic acid. During the deep freezing period, the SOD activity and glutathione (GSH) content of the winter wheat varieties were relative to their cold resistance. During this period, the MDA stability, SOD, GSH, and the total water and the free water content might be used to identify the cold resistance of winter wheat varieties. 展开更多
关键词 winter wheat cold resistance cell viability protective enzyme plasma membrane peroxidation water
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Componentwise Asymptotic Stability of Continuous-Time Interval Systems
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作者 赵胜民 唐万生 +1 位作者 李光泉 李文秀 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2003年第4期34-38,共5页
A special type of asymptotic (exponential) stability, namely componentwise asymptotic (exponential) stability for the continuous-time interval system is investigated. A set-valued map that represents the constraint of... A special type of asymptotic (exponential) stability, namely componentwise asymptotic (exponential) stability for the continuous-time interval system is investigated. A set-valued map that represents the constraint of the state of the system is defined. And, by applying the viability theory of differential equation, sufficient and necessary conditions for the componentwise asymptotical (exponential) stability of this kind of systems are given. 展开更多
关键词 interval system componentwise stability viability theory.
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