Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were d...Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism （RFLP） analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria （71%） and Bacillus （23%）. Scanning electron micrographs revealed that there were abundant rod and coceoidal forms encased in sulphur and sodium chloride precipitate. These results revealed that there were a diversity of moderate thermophilic bacterial populations thrived in Lau Basin hydrothermal vents that were previously not detected by either molecular retrieval or strain purification techniques.展开更多
The effects of vents on thermal ventilation to save energy in the cold rolling workshop of Baosteel were investigated.According to the scale modeling theory,a small chamber was established.The details about constructi...The effects of vents on thermal ventilation to save energy in the cold rolling workshop of Baosteel were investigated.According to the scale modeling theory,a small chamber was established.The details about construction of experiment on thermal ventilation and the preparation and arrangement of apparatus were discussed,and then the effects of vents on thermal ventilation were studied through experiments,which includes the temperature distribution,the volume of ventilation,the temperature difference between inlets and outlets,the neutral plane,and the effective thermal coefficient of thermal natural ventilation.Based on this,the effects of natural ventilation based on varied area of inlets and outlets and those of vents on one side and on different sides were compared.According to the experiments,the area of inlet vents and outlet vents affect the temperature distribution in chamber,and their effects on ventilation volume are different,but the effects of vents in single side or different sides are the same under the condition that only thermal ventilation is considered.展开更多
RBOE is a new type of DNAN-based high-energy melt-cast mixed explosive,whose safety under thermal stimulation is significantly affected by heating conditions and venting area of the warhead.Based on the thermal decomp...RBOE is a new type of DNAN-based high-energy melt-cast mixed explosive,whose safety under thermal stimulation is significantly affected by heating conditions and venting area of the warhead.Based on the thermal decomposition reaction characteristics and combustion characteristics of each component of RBOE explosive,the cook-off calculation models of RBOE warhead before and after ignition were established.In addition,closed and vented warheads were designed,as well as fast and slow cook-off test devices.The cook-off characteristics and thermal safety venting area of RBOE warhead were extensively studied.The results showed that the closed RBOE warhead underwent deflagration reaction under both slow and fast cook-off conditions.The calculation result of the shell wall temperature before slow cookoff ignition response of the warhead was 454.06 K,with an error of+1.75%compared to the test result of462.15 K,and the temperature rise rate calculated was in good agreement with the test.The calculated ignition time of RBOE warhead under fast cook-off was 161 s,with an error of+8.8%compared to the test result of 148 s,which verified the accuracy of cook-off model of RBOE warhead before ignition.According to the cook-off calculation model of the warhead after ignition and cook-off test of the vented warhead,it was determined that the thermal safety venting area was 1124.61 mm^(2)for fast cook-off and 530.66 mm~2 for slow cook-off,effectively preventing the reaction of warhead above combustion.Therefore,this study provides a scientific basis for the thermal safety design and evaluation of insensitive warheads.展开更多
The internal and external flow fields during vented explosions of methane were characterized through numerical simulation,and the capability of numerical simulation thereof was validated by previous experimental data ...The internal and external flow fields during vented explosions of methane were characterized through numerical simulation,and the capability of numerical simulation thereof was validated by previous experimental data at three ignition positions.The venting mechanism was revealed by the simulated concentration distribution,temperature profile,and airflow velocity.The results show rear ignition results in the external methane mass distribution taking the form of"mushroom"and columnar flames in the external space,which can be expressed as a third-order polynomial relationship with distance;central ignition forms a relationship of the form y=AxB.Front ignition causes the temperature to show a tendency to repeated oscillations(rising,falling,and rising).Central ignition generates the maximum vented airflow velocity(V_(max)=320 m/s)upon vent opening.The results indicate that it is acceptable to apply numerical simulation of methane explosions in practice.展开更多
To research the characteristics of vented explosion of methane-air mixture in the pipeline,coal mine tunnel or other closed space,the experiments and numerical simulations were carried out.In this work,explosion chara...To research the characteristics of vented explosion of methane-air mixture in the pipeline,coal mine tunnel or other closed space,the experiments and numerical simulations were carried out.In this work,explosion characteristics and flame propagation characteristics of methane in pipeline and coal mine tunnel are studied by using an explosion test system,combined with FLACS software,under different vented conditions.The numerical simulation results of methane explosion are basically consistent with the physical experiment results,which indicates that the numerical simulation for methane explosion is reliable to be applied to the practice.The results show that explosion parameters(pressure,temperature and product concentration)of methane at five volume fractions have the same change trend.Nevertheless,the explosion intension of 10.0%methane is the largest and that of 9.5%methane is relatively weak,followed by 11.0%methane,8.0%methane and 7.0%methane respectively.Under different vented conditions,the pressure and temperature of methane explosion are the highest in the pipeline without a vent,followed by the pipeline where ignition or vent position is in each end,and those are the lowest in the pipeline with ignition and vent at the same end.There is no significant effect on final product concentration of methane explosion under three vented conditions.For coal mine tunnel,it is indicated that the maximum explosion pressure at the airproof wall in return airway with the branch roadway at 50 m from goaf is significantly decreased while that in intake airway does not change overwhelmingly.In addition,when the branch roadway is longer or its section is larger,the peak pressure of airproof wall reduces slightly.展开更多
基金Project(200805032) supported by Grands from Scientific Research Program of Marine Public Welfare Industry of ChinaProject (DYXM-115-02-2-07) supported by China Ocean Mineral Resources R&D Association (COMRA)
文摘Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 ℃ and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism （RFLP） analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria （71%） and Bacillus （23%）. Scanning electron micrographs revealed that there were abundant rod and coceoidal forms encased in sulphur and sodium chloride precipitate. These results revealed that there were a diversity of moderate thermophilic bacterial populations thrived in Lau Basin hydrothermal vents that were previously not detected by either molecular retrieval or strain purification techniques.
文摘The effects of vents on thermal ventilation to save energy in the cold rolling workshop of Baosteel were investigated.According to the scale modeling theory,a small chamber was established.The details about construction of experiment on thermal ventilation and the preparation and arrangement of apparatus were discussed,and then the effects of vents on thermal ventilation were studied through experiments,which includes the temperature distribution,the volume of ventilation,the temperature difference between inlets and outlets,the neutral plane,and the effective thermal coefficient of thermal natural ventilation.Based on this,the effects of natural ventilation based on varied area of inlets and outlets and those of vents on one side and on different sides were compared.According to the experiments,the area of inlet vents and outlet vents affect the temperature distribution in chamber,and their effects on ventilation volume are different,but the effects of vents in single side or different sides are the same under the condition that only thermal ventilation is considered.
基金National Natural Science Foundation of china(Grant No.12402468)。
文摘RBOE is a new type of DNAN-based high-energy melt-cast mixed explosive,whose safety under thermal stimulation is significantly affected by heating conditions and venting area of the warhead.Based on the thermal decomposition reaction characteristics and combustion characteristics of each component of RBOE explosive,the cook-off calculation models of RBOE warhead before and after ignition were established.In addition,closed and vented warheads were designed,as well as fast and slow cook-off test devices.The cook-off characteristics and thermal safety venting area of RBOE warhead were extensively studied.The results showed that the closed RBOE warhead underwent deflagration reaction under both slow and fast cook-off conditions.The calculation result of the shell wall temperature before slow cookoff ignition response of the warhead was 454.06 K,with an error of+1.75%compared to the test result of462.15 K,and the temperature rise rate calculated was in good agreement with the test.The calculated ignition time of RBOE warhead under fast cook-off was 161 s,with an error of+8.8%compared to the test result of 148 s,which verified the accuracy of cook-off model of RBOE warhead before ignition.According to the cook-off calculation model of the warhead after ignition and cook-off test of the vented warhead,it was determined that the thermal safety venting area was 1124.61 mm^(2)for fast cook-off and 530.66 mm~2 for slow cook-off,effectively preventing the reaction of warhead above combustion.Therefore,this study provides a scientific basis for the thermal safety design and evaluation of insensitive warheads.
基金supported by the Young Scientists Fund of National Natural Science Foundation of China(Grant Nos.12202202 and 12202494)the National Key Research and Development Program of China(Grant No.2021YFC3100700)。
文摘The internal and external flow fields during vented explosions of methane were characterized through numerical simulation,and the capability of numerical simulation thereof was validated by previous experimental data at three ignition positions.The venting mechanism was revealed by the simulated concentration distribution,temperature profile,and airflow velocity.The results show rear ignition results in the external methane mass distribution taking the form of"mushroom"and columnar flames in the external space,which can be expressed as a third-order polynomial relationship with distance;central ignition forms a relationship of the form y=AxB.Front ignition causes the temperature to show a tendency to repeated oscillations(rising,falling,and rising).Central ignition generates the maximum vented airflow velocity(V_(max)=320 m/s)upon vent opening.The results indicate that it is acceptable to apply numerical simulation of methane explosions in practice.
基金Project(51674193)supported by the National Natural Science Foundation of ChinaProject(2019-JLM-9)supported by the Natural Science Foundation of Shaanxi Province,ChinaProject(2019-M-663780)supported by the Postdoctoral Science Foundation,China。
文摘To research the characteristics of vented explosion of methane-air mixture in the pipeline,coal mine tunnel or other closed space,the experiments and numerical simulations were carried out.In this work,explosion characteristics and flame propagation characteristics of methane in pipeline and coal mine tunnel are studied by using an explosion test system,combined with FLACS software,under different vented conditions.The numerical simulation results of methane explosion are basically consistent with the physical experiment results,which indicates that the numerical simulation for methane explosion is reliable to be applied to the practice.The results show that explosion parameters(pressure,temperature and product concentration)of methane at five volume fractions have the same change trend.Nevertheless,the explosion intension of 10.0%methane is the largest and that of 9.5%methane is relatively weak,followed by 11.0%methane,8.0%methane and 7.0%methane respectively.Under different vented conditions,the pressure and temperature of methane explosion are the highest in the pipeline without a vent,followed by the pipeline where ignition or vent position is in each end,and those are the lowest in the pipeline with ignition and vent at the same end.There is no significant effect on final product concentration of methane explosion under three vented conditions.For coal mine tunnel,it is indicated that the maximum explosion pressure at the airproof wall in return airway with the branch roadway at 50 m from goaf is significantly decreased while that in intake airway does not change overwhelmingly.In addition,when the branch roadway is longer or its section is larger,the peak pressure of airproof wall reduces slightly.
