An improved method for showing Gc band by sulfosalicylic acidafter IEF is reported. This method provides us an effective mean to identify theGc subtypes and its variants within 4 h without using anti-serum for immunof...An improved method for showing Gc band by sulfosalicylic acidafter IEF is reported. This method provides us an effective mean to identify theGc subtypes and its variants within 4 h without using anti-serum for immunofix-ation. And new variants Gc<sup>1c3</sup> and Gc<sup>2c7</sup> are discovered in Chinese people, andtheir frequencies are 0. 0008 and 0. 0004 respectively.展开更多
OBJECTIVE The specificity of drug therapy in individuals and races has promoted the development and improvement of pharmacogenomics and precision medicine.While there is a few cognition on the minorities in China,espe...OBJECTIVE The specificity of drug therapy in individuals and races has promoted the development and improvement of pharmacogenomics and precision medicine.While there is a few cognition on the minorities in China,especially in Lisu nationality from the Yunnan province.Therefore,we performed the research to improve the role of pharmacogenomics in the Lisu population from the Yunnan province of China.METHODS 54 variants of very important pharmacogenes selected from the PharmGKB database were genotyped in 199 unrelated and healthy Lisu adults from the Yunnan province of China,and then,genotyping data wtih χ^(2) test were analyzed.RESULTS We compared our data with those of other 26 populations from the 1000 Genomes Project,and acquired that the Lisu ethnicity is similar with the CDX(Chinese Dai in Xishuangbanna,China)and CHS(Southern Han Chinese,China).Furthermore,rs776746(CYP3A5),rs1805123(KCNH2),rs4291(ACE),rs1051298(SLC19A1)and rs1065852(CYP2D6)were deemed as the most varying loci.The MAF of“G”at rs1805123(KCNH2)in the Lisu population was the largest with the value of 51.0%.CONCLUSION There are significant differences in single nucleotide polymorphism loci,supplementing the pharmacogenomic information of the Lisu population in Yunnan province,China,and can provide a theoretical basis for individualized medication in the future.展开更多
Physiological and genetic variations exist richlyin the cotton plants regenerated from somaticembryo.A stable homeotic variant(chvl)ofcotton from regenerated plants was identified.Using light microscope and scanning e...Physiological and genetic variations exist richlyin the cotton plants regenerated from somaticembryo.A stable homeotic variant(chvl)ofcotton from regenerated plants was identified.Using light microscope and scanning electronmicroscope to investigate the morphologicalchanges in chvl,the results revealed that all thefloral organs in the chvl plants converted展开更多
Based on introduction characteristics and classific at ion of Mass Customization (MC), the paper expounds foundations and design me thods of MC. The foundations of MC production are standardization, seriation and norm...Based on introduction characteristics and classific at ion of Mass Customization (MC), the paper expounds foundations and design me thods of MC. The foundations of MC production are standardization, seriation and normality of production, guiding thought of Concurrent Engineering (CE), ap plication of CAD/CAM technology and etc. And design methods of MC products are d esign for product family, modular design, parameterization CAD, variant design, design for manufacturing (DFM) and design by customers. Design for product famil y is a method that first to design a basic form of the product, and then to with draw variant parameters to become a variable product model, makes a family or a series of product design at the same time. Modular design is that based on produ ct function analysis of different function or the same function but different sp ecification within a certain scope, divides and designs out a series of function modular, and through selection and combination of the modular can make up diffe rent products. Parameterization CAD is a CAD method that has function of dimensi on drive. Variant design is a method that under circumstance of keeping principl e constant and construction similar or likeness, properly adjusts part of constr uction and design parameters to adapt design demands. DFM demands the designer t o consider problems that will appear in manufacture in the stage of design, and to avoid them through optimal design. And design by customers is guiding custome rs to design within the scope of production capacity of enterprise and optional items that enterprise has designed to satisfy their requirements of form, functi on and price. Among them modular design, parameterization CAD and variant design are key technology of design methods of MC products. And design by customers is a main characteristic of design of customization product, and an effective sale means also.展开更多
Toll-like receptor （TLR） 4 plays an important role in the innate immune system and has been involved in resistance/ susceptibility to a number of diseases as revealed by studies in human and other domestic animals. ...Toll-like receptor （TLR） 4 plays an important role in the innate immune system and has been involved in resistance/ susceptibility to a number of diseases as revealed by studies in human and other domestic animals. Wild boar survives in natural environment without artificial interference and may be different from domestic pig in innate immune system. Here, the complete coding sequence of TLR4 and TLR4A was cloned in wild boar, and two other alternative splicing variants, TLR4B and TLR4C, were obtained. Compared to the counterpart from domestic pig （GcnBank No. AJ628065）, there were five SNPs, c.510T〉C, c.960A〉G, c.962A〉G, c.1605T〉G and c.1824A〉G, in the coding sequence of wild boar TLR4A gene. TLR4 gene was expressed in all the tissues from wild boar studied with the most abundance in spleen tissue, and mRNA level was significantly lower in spleen from wild boar than that from Min pig. The allele distribution was significantly different at polymorphic loci c.962G〉A and c.1027C〉A （p〈0.01） between wild boar and Min pig. The results would contribute to understand the innate immune system in wild boar.展开更多
文摘An improved method for showing Gc band by sulfosalicylic acidafter IEF is reported. This method provides us an effective mean to identify theGc subtypes and its variants within 4 h without using anti-serum for immunofix-ation. And new variants Gc<sup>1c3</sup> and Gc<sup>2c7</sup> are discovered in Chinese people, andtheir frequencies are 0. 0008 and 0. 0004 respectively.
文摘OBJECTIVE The specificity of drug therapy in individuals and races has promoted the development and improvement of pharmacogenomics and precision medicine.While there is a few cognition on the minorities in China,especially in Lisu nationality from the Yunnan province.Therefore,we performed the research to improve the role of pharmacogenomics in the Lisu population from the Yunnan province of China.METHODS 54 variants of very important pharmacogenes selected from the PharmGKB database were genotyped in 199 unrelated and healthy Lisu adults from the Yunnan province of China,and then,genotyping data wtih χ^(2) test were analyzed.RESULTS We compared our data with those of other 26 populations from the 1000 Genomes Project,and acquired that the Lisu ethnicity is similar with the CDX(Chinese Dai in Xishuangbanna,China)and CHS(Southern Han Chinese,China).Furthermore,rs776746(CYP3A5),rs1805123(KCNH2),rs4291(ACE),rs1051298(SLC19A1)and rs1065852(CYP2D6)were deemed as the most varying loci.The MAF of“G”at rs1805123(KCNH2)in the Lisu population was the largest with the value of 51.0%.CONCLUSION There are significant differences in single nucleotide polymorphism loci,supplementing the pharmacogenomic information of the Lisu population in Yunnan province,China,and can provide a theoretical basis for individualized medication in the future.
文摘Physiological and genetic variations exist richlyin the cotton plants regenerated from somaticembryo.A stable homeotic variant(chvl)ofcotton from regenerated plants was identified.Using light microscope and scanning electronmicroscope to investigate the morphologicalchanges in chvl,the results revealed that all thefloral organs in the chvl plants converted
文摘Based on introduction characteristics and classific at ion of Mass Customization (MC), the paper expounds foundations and design me thods of MC. The foundations of MC production are standardization, seriation and normality of production, guiding thought of Concurrent Engineering (CE), ap plication of CAD/CAM technology and etc. And design methods of MC products are d esign for product family, modular design, parameterization CAD, variant design, design for manufacturing (DFM) and design by customers. Design for product famil y is a method that first to design a basic form of the product, and then to with draw variant parameters to become a variable product model, makes a family or a series of product design at the same time. Modular design is that based on produ ct function analysis of different function or the same function but different sp ecification within a certain scope, divides and designs out a series of function modular, and through selection and combination of the modular can make up diffe rent products. Parameterization CAD is a CAD method that has function of dimensi on drive. Variant design is a method that under circumstance of keeping principl e constant and construction similar or likeness, properly adjusts part of constr uction and design parameters to adapt design demands. DFM demands the designer t o consider problems that will appear in manufacture in the stage of design, and to avoid them through optimal design. And design by customers is guiding custome rs to design within the scope of production capacity of enterprise and optional items that enterprise has designed to satisfy their requirements of form, functi on and price. Among them modular design, parameterization CAD and variant design are key technology of design methods of MC products. And design by customers is a main characteristic of design of customization product, and an effective sale means also.
基金Supported by the National Natural Science Foundation of China(31072007)China Agriculture Research System(CARS-36)
文摘Toll-like receptor （TLR） 4 plays an important role in the innate immune system and has been involved in resistance/ susceptibility to a number of diseases as revealed by studies in human and other domestic animals. Wild boar survives in natural environment without artificial interference and may be different from domestic pig in innate immune system. Here, the complete coding sequence of TLR4 and TLR4A was cloned in wild boar, and two other alternative splicing variants, TLR4B and TLR4C, were obtained. Compared to the counterpart from domestic pig （GcnBank No. AJ628065）, there were five SNPs, c.510T〉C, c.960A〉G, c.962A〉G, c.1605T〉G and c.1824A〉G, in the coding sequence of wild boar TLR4A gene. TLR4 gene was expressed in all the tissues from wild boar studied with the most abundance in spleen tissue, and mRNA level was significantly lower in spleen from wild boar than that from Min pig. The allele distribution was significantly different at polymorphic loci c.962G〉A and c.1027C〉A （p〈0.01） between wild boar and Min pig. The results would contribute to understand the innate immune system in wild boar.
