Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In ...Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers.The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 min.After the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker selection.In the projection of positive mode,DM/DN patients and the controls were obviously divided into three groups.Furthermore,two potential markers were identified as phytosphingosine and sphinganine,respectively.Phytosphingosine and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid metabolism.展开更多
采用质子传递反应-飞行时间质谱仪(Proton Transfer Reaction-Time of Flight-Mass Spectrometry,PTR-TOFMS)与气相色谱-质谱联用技术(Gas Chromatography-Mass Spectrometer,GC-MS)对不同品种武夷岩茶(水仙、肉桂)香气成分进行分析,结...采用质子传递反应-飞行时间质谱仪(Proton Transfer Reaction-Time of Flight-Mass Spectrometry,PTR-TOFMS)与气相色谱-质谱联用技术(Gas Chromatography-Mass Spectrometer,GC-MS)对不同品种武夷岩茶(水仙、肉桂)香气成分进行分析,结果表明:武夷水仙以反式橙花叔醇、(E,E)-2,4-庚二烯醛、芳樟醇及其氧化物等为主;武夷肉桂是反式橙花叔醇、α-法尼烯、苯甲醛为主,且在武夷山肉桂中存在高浓度的m/z 207、223、281、291几个未知化合物。2种分析技术所检测到的香气成分和相对含量比例差异显著,两者各有优劣,相互补充,联合分析更加完整,更有利于准确地进行香气分析,同时发现PTR-TOF-MS法更能真实地反映茶叶香气的完整信息。展开更多
文摘Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers.The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 min.After the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker selection.In the projection of positive mode,DM/DN patients and the controls were obviously divided into three groups.Furthermore,two potential markers were identified as phytosphingosine and sphinganine,respectively.Phytosphingosine and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid metabolism.
文摘采用质子传递反应-飞行时间质谱仪(Proton Transfer Reaction-Time of Flight-Mass Spectrometry,PTR-TOFMS)与气相色谱-质谱联用技术(Gas Chromatography-Mass Spectrometer,GC-MS)对不同品种武夷岩茶(水仙、肉桂)香气成分进行分析,结果表明:武夷水仙以反式橙花叔醇、(E,E)-2,4-庚二烯醛、芳樟醇及其氧化物等为主;武夷肉桂是反式橙花叔醇、α-法尼烯、苯甲醛为主,且在武夷山肉桂中存在高浓度的m/z 207、223、281、291几个未知化合物。2种分析技术所检测到的香气成分和相对含量比例差异显著,两者各有优劣,相互补充,联合分析更加完整,更有利于准确地进行香气分析,同时发现PTR-TOF-MS法更能真实地反映茶叶香气的完整信息。
