Lead(Pb)and zinc(Zn)are widely recognized as common environmental contaminants,contributing to soil degradation and posing risks to environmental health.Combining functional carbon-based materials with microorganisms ...Lead(Pb)and zinc(Zn)are widely recognized as common environmental contaminants,contributing to soil degradation and posing risks to environmental health.Combining functional carbon-based materials with microorganisms has been considered as an effective and environmentally friendly strategy for remediating Pb/Zn-contaminated soil.However,there is still a lack of understanding the connection between heavy metal immobilization and plant responses,which hampers practical applications.Here,a 90-day pot experiment was conducted to investigate the integrated effects of biochar(WS700)and microorganisms including inorganic phosphate-solubilizing bacteria(IPSB)and sulfate reducing bacteria(SRB)on Pb and Zn synchronous immobilization and the physiological responses of Brassica rapa var.chinensis(Brassica).Compared with CK,bacteria-loaded biochar treatment declined the exchangeable Pb and Zn fraction by 94.69%−98.37%and 94.55%−99.52%,while increasing the residual state Pb and Zn by 75.50%−208.58%and 96.71%−110.85%,respectively.Three amendments enhanced Brassica growth by improving total chlorophyll content and superoxide dismutase(SOD)and peroxidase(POD)activities.The bacteria-loaded biochar treatment effectively regulated stomatal conductance and reduced intercellular CO_(2) concentration.Moreover,compared with CK,three amendments reduced MDA content by 28.84%,28.30%and 41.60%,respectively,under the high concentration of Pb and Zn.The findings demonstrated the significant role of bacterial-biochar consortia in immobilizing Pb and Zn and mitigating Pb and Zn-induced stress in plants by regulating photosynthetic characteristics and antioxidant enzyme activities.展开更多
Rare earth has a long history of agricultural use and can improve the yield and quality of agricultural by-products,but its regulation mechanism is still unclear.The aim of the study was to investigate the effects of ...Rare earth has a long history of agricultural use and can improve the yield and quality of agricultural by-products,but its regulation mechanism is still unclear.The aim of the study was to investigate the effects of lanthanum and cerium on the activities of peroxidase,catalase and ascorbate peroxidase in leaves of soybeans[Glycine max(L.)Merr].Soybean varieties of Dongnong 42,Dongnong 47 and Dongnong 52 from Northeast China were used as experimental materials.The samples were tested by spraying different concentrations of LaCl3(120,150 and 180 mg·L-1),CeCl3(30,60 and 90 mg·L-1)and LaCl3+CeCl3(30,40 and60 mg·L-1 mix)solutions on the leaves at the seedling stage and the early flowering stage of soybeans.The results showed that the suitable species and concentrations of lanthanum and cerium could increased the activities of antioxidant enzymes in soybean leaves,but the effects of lanthanum and cerium on different varieties of soybeans were also different.For Dongnong 42,the optimum lanthanum and cerium treatment was 60 mg·L-1 LaCl3+CeCl3,and the activities of peroxidase,catalase and ascorbate peroxidase increased by 11.36%(p<0.05),13.89%(p>0.05)and 50.00%(p<0.05)compared with those of CK.The optimal lanthanum and cerium treatment of Dongnong 47 was40 mg·L-1 LaCl3+CeCl3.Compared with those of CK,the above three enzyme activities increased by 15.80%(p<0.05),51.52%(p<0.05)and 20.75%(p>0.05).The optimal lanthanum and cerium treatment of Dongnong 52 was 60 mg·L-1 LaCl3+CeCl3,which increased the activities of the three enzymes by 33.49%(p<0.05),19.23%(p>0.05)and 23.53%(p<0.05)compared with those of CK.展开更多
Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of N...Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism.展开更多
基金Projects(2019NY-200,2020ZDLNY06-06,2020ZDLNY07-10)supported by the Key Research and Development Program of Shaanxi Province,ChinaProject(2019YFC1803604)supported by the National Key Research and Development Program of China。
文摘Lead(Pb)and zinc(Zn)are widely recognized as common environmental contaminants,contributing to soil degradation and posing risks to environmental health.Combining functional carbon-based materials with microorganisms has been considered as an effective and environmentally friendly strategy for remediating Pb/Zn-contaminated soil.However,there is still a lack of understanding the connection between heavy metal immobilization and plant responses,which hampers practical applications.Here,a 90-day pot experiment was conducted to investigate the integrated effects of biochar(WS700)and microorganisms including inorganic phosphate-solubilizing bacteria(IPSB)and sulfate reducing bacteria(SRB)on Pb and Zn synchronous immobilization and the physiological responses of Brassica rapa var.chinensis(Brassica).Compared with CK,bacteria-loaded biochar treatment declined the exchangeable Pb and Zn fraction by 94.69%−98.37%and 94.55%−99.52%,while increasing the residual state Pb and Zn by 75.50%−208.58%and 96.71%−110.85%,respectively.Three amendments enhanced Brassica growth by improving total chlorophyll content and superoxide dismutase(SOD)and peroxidase(POD)activities.The bacteria-loaded biochar treatment effectively regulated stomatal conductance and reduced intercellular CO_(2) concentration.Moreover,compared with CK,three amendments reduced MDA content by 28.84%,28.30%and 41.60%,respectively,under the high concentration of Pb and Zn.The findings demonstrated the significant role of bacterial-biochar consortia in immobilizing Pb and Zn and mitigating Pb and Zn-induced stress in plants by regulating photosynthetic characteristics and antioxidant enzyme activities.
基金Supported by the National Natural Science Foundation of China(31471440)。
文摘Rare earth has a long history of agricultural use and can improve the yield and quality of agricultural by-products,but its regulation mechanism is still unclear.The aim of the study was to investigate the effects of lanthanum and cerium on the activities of peroxidase,catalase and ascorbate peroxidase in leaves of soybeans[Glycine max(L.)Merr].Soybean varieties of Dongnong 42,Dongnong 47 and Dongnong 52 from Northeast China were used as experimental materials.The samples were tested by spraying different concentrations of LaCl3(120,150 and 180 mg·L-1),CeCl3(30,60 and 90 mg·L-1)and LaCl3+CeCl3(30,40 and60 mg·L-1 mix)solutions on the leaves at the seedling stage and the early flowering stage of soybeans.The results showed that the suitable species and concentrations of lanthanum and cerium could increased the activities of antioxidant enzymes in soybean leaves,but the effects of lanthanum and cerium on different varieties of soybeans were also different.For Dongnong 42,the optimum lanthanum and cerium treatment was 60 mg·L-1 LaCl3+CeCl3,and the activities of peroxidase,catalase and ascorbate peroxidase increased by 11.36%(p<0.05),13.89%(p>0.05)and 50.00%(p<0.05)compared with those of CK.The optimal lanthanum and cerium treatment of Dongnong 47 was40 mg·L-1 LaCl3+CeCl3.Compared with those of CK,the above three enzyme activities increased by 15.80%(p<0.05),51.52%(p<0.05)and 20.75%(p>0.05).The optimal lanthanum and cerium treatment of Dongnong 52 was 60 mg·L-1 LaCl3+CeCl3,which increased the activities of the three enzymes by 33.49%(p<0.05),19.23%(p>0.05)and 23.53%(p<0.05)compared with those of CK.
基金Supported by the Natural Science Foundation of Heilongjiang Province(LH2019C021)。
文摘Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism.
