bZIP transcription factor family is one of the largest groups of the plant transcription factor families and plays an important role in plant growth and adaption to the abiotic stresses. In this study, two AtbZIP1 mut...bZIP transcription factor family is one of the largest groups of the plant transcription factor families and plays an important role in plant growth and adaption to the abiotic stresses. In this study, two AtbZIP1 mutant Arabidopsis （bzipl） were used with T-DNA inserted into two different sites, designated as SALK-556773 and SALK-660942, in order to identify different effects on AtbZIP1 gene expression by different T-DNA insertion sites. PCR and RT-PCR results revealed that T-DNA insertion in CDS region could effectively inhibit AtbZIP1 gene expression, while T-DNA insertion in 3＇-UTR couldn＇t. The phenotype analysis further confirmed the differences and showed that T-DNA insertion in CDS region decreased plants＇ drought resistance, while in 3＇-UTR couldn＇t. The phenotype assays also suggested that AtbZIP1 held pivotal roles in plant response to drought stress.展开更多
The mechanistic basis of cellulose biosynthesis in plants has gained ground during last decade or so.The isolation of plant cDNA clones encoding cotton homologs of the bacterial cellulose
Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Col0) was transformed by spraying Agrobacterium tumefacience with Tri...Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Col0) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase (ThEn-42) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86.7%) 10 days after inoculation with the pathogen.展开更多
为了探究Mn_(3)O_(4)纳米酶对改善盐胁迫下拟南芥的耐受作用,文章通过水热法合成Mn_(3)O_(4)纳米酶,采用透射电子显微镜(transmission electron microscopy,TEM)对Mn_(3)O_(4)纳米酶的形貌进行表征,测定Mn_(3)O_(4)纳米酶活性,并进一步...为了探究Mn_(3)O_(4)纳米酶对改善盐胁迫下拟南芥的耐受作用,文章通过水热法合成Mn_(3)O_(4)纳米酶,采用透射电子显微镜(transmission electron microscopy,TEM)对Mn_(3)O_(4)纳米酶的形貌进行表征,测定Mn_(3)O_(4)纳米酶活性,并进一步研究Mn_(3)O_(4)纳米酶在盐胁迫下对拟南芥生长及光合作用的影响。结果表明:合成的Mn_(3)O_(4)纳米酶尺寸为10nm左右;Mn_(3)O_(4)纳米酶对H_(2)O_(2)、羟基自由基(·OH)及超氧阴离子自由基(·O_(2)^(-))等多种活性氧(reactive oxygen species,ROS)具有清除能力;施加Mn_(3)O_(4)纳米酶可以改善盐胁迫对拟南芥生长的抑制作用及光合作用。研究结果可为解决盐胁迫下抑制植物的生长提供一种解决方案。展开更多
基金Supported by National Natural Science Foundation of China (30570990)National Major Project for Cultivation of Transgenic Crops (20082x08004)+1 种基金Key Research Plan of Heilongjiang Province (GA06B103)Innovation Research Group of NEAU (CXT004)
文摘bZIP transcription factor family is one of the largest groups of the plant transcription factor families and plays an important role in plant growth and adaption to the abiotic stresses. In this study, two AtbZIP1 mutant Arabidopsis （bzipl） were used with T-DNA inserted into two different sites, designated as SALK-556773 and SALK-660942, in order to identify different effects on AtbZIP1 gene expression by different T-DNA insertion sites. PCR and RT-PCR results revealed that T-DNA insertion in CDS region could effectively inhibit AtbZIP1 gene expression, while T-DNA insertion in 3＇-UTR couldn＇t. The phenotype analysis further confirmed the differences and showed that T-DNA insertion in CDS region decreased plants＇ drought resistance, while in 3＇-UTR couldn＇t. The phenotype assays also suggested that AtbZIP1 held pivotal roles in plant response to drought stress.
文摘The mechanistic basis of cellulose biosynthesis in plants has gained ground during last decade or so.The isolation of plant cDNA clones encoding cotton homologs of the bacterial cellulose
文摘Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Col0) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase (ThEn-42) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86.7%) 10 days after inoculation with the pathogen.
文摘为了探究Mn_(3)O_(4)纳米酶对改善盐胁迫下拟南芥的耐受作用,文章通过水热法合成Mn_(3)O_(4)纳米酶,采用透射电子显微镜(transmission electron microscopy,TEM)对Mn_(3)O_(4)纳米酶的形貌进行表征,测定Mn_(3)O_(4)纳米酶活性,并进一步研究Mn_(3)O_(4)纳米酶在盐胁迫下对拟南芥生长及光合作用的影响。结果表明:合成的Mn_(3)O_(4)纳米酶尺寸为10nm左右;Mn_(3)O_(4)纳米酶对H_(2)O_(2)、羟基自由基(·OH)及超氧阴离子自由基(·O_(2)^(-))等多种活性氧(reactive oxygen species,ROS)具有清除能力;施加Mn_(3)O_(4)纳米酶可以改善盐胁迫对拟南芥生长的抑制作用及光合作用。研究结果可为解决盐胁迫下抑制植物的生长提供一种解决方案。
