In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(...In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(PPO) were investigated,and the contents of soluble protein,sugar and malondialdehyde(MDA) were determined in fresh tea leaves in the autumn.The results showed that the activities of POD and the content of soluble sugar were both much higher in the variety with strong cold resistance,and the content of soluble protein was associated with the cold resistance,while the cold resistance of the plant was not related with the activities of the CAT,SOD and PPO,and the content of MDA.It is considered that the activity of POD and contents of soluble sugar could be used as indicators to identify the cold resistance of tea varieties.展开更多
Tea plant absorbs from soil a large amount of fluorine(F) which is accumulated in leaf,resulting in elevated F concentration in tea.It is a controversial topic whether the fluoride in tea is associated with fluorosis....Tea plant absorbs from soil a large amount of fluorine(F) which is accumulated in leaf,resulting in elevated F concentration in tea.It is a controversial topic whether the fluoride in tea is associated with fluorosis.The research advances in F variation of various kinds of tea,factors influencing F level in tea and the associated health risk assessment were reviewed in the present paper.The F level in tea increases with the maturity of the tea leaf.Brick tea had a high F content because it was prepared using mature shoots and old tea leaves as raw material.Some low grade UK supermarket economy teas contained elevated F comparable to the Chinese brick tea.Tea F level is also affected by tea cultivar,soil condition,tea processing method and tea infusion preparation method.展开更多
为挖掘乌龙茶品种鲜叶的特征特性,以绿茶品种‘福鼎大毫茶’一芽二叶或三叶为对照(CK),分析比较了高香优质乌龙茶品种‘茗科1号’一芽二叶或三叶(TM)与中小开面二至四叶(MM)在转录和代谢水平的组成差异。结果表明,茶树品种特性和采摘标...为挖掘乌龙茶品种鲜叶的特征特性,以绿茶品种‘福鼎大毫茶’一芽二叶或三叶为对照(CK),分析比较了高香优质乌龙茶品种‘茗科1号’一芽二叶或三叶(TM)与中小开面二至四叶(MM)在转录和代谢水平的组成差异。结果表明,茶树品种特性和采摘标准为影响茶鲜叶转录和代谢轮廓的重要因素,其中CK、TM和MM的转录与代谢轮廓相互间均有良好的模式区分。差异表达基因与差异代谢物的富集和关联分析结果表明,TM vs CK与MM vs TM的差异表达基因在分子功能、细胞组分和生物过程方面存在不同的GO功能富集模式,且前者拥有较多的生物过程和较少的细胞组分;“植物次生代谢产物的生物合成”、“精氨酸和脯氨酸代谢”与“类胡萝卜素生物合成”、“苯丙素的生物合成”、“甘氨酸、丝氨酸和苏氨酸代谢”分别是TM vsCK、MM vs TM在2种组学分析中的前20条共有KEGG富集代谢通路。TM vs CK中L-苯丙氨酸、反式肉桂酸和4-胍基丁酸丰度的显著降低,以及MM vs TM中胆碱丰度的显著提高和L-苯丙氨酸、L-色氨酸、L-高丝氨酸丰度的显著降低均与相应共富集代谢通路中多个基因的显著上调或下调表达密切相关。此外,MM vs TM中脱落酸丰度的显著提高与“类胡萝卜素生物合成”通路中紫黄质脱环氧化酶和番茄红素β-环化酶基因的显著上调表达高度相关。这可为阐明‘茗科1号’茶鲜叶生物学特征及其多茶类兼制特性提供参考依据。展开更多
文摘In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(PPO) were investigated,and the contents of soluble protein,sugar and malondialdehyde(MDA) were determined in fresh tea leaves in the autumn.The results showed that the activities of POD and the content of soluble sugar were both much higher in the variety with strong cold resistance,and the content of soluble protein was associated with the cold resistance,while the cold resistance of the plant was not related with the activities of the CAT,SOD and PPO,and the content of MDA.It is considered that the activity of POD and contents of soluble sugar could be used as indicators to identify the cold resistance of tea varieties.
基金financially supported by the National Science Foundation of China (Project No.31170643)
文摘Tea plant absorbs from soil a large amount of fluorine(F) which is accumulated in leaf,resulting in elevated F concentration in tea.It is a controversial topic whether the fluoride in tea is associated with fluorosis.The research advances in F variation of various kinds of tea,factors influencing F level in tea and the associated health risk assessment were reviewed in the present paper.The F level in tea increases with the maturity of the tea leaf.Brick tea had a high F content because it was prepared using mature shoots and old tea leaves as raw material.Some low grade UK supermarket economy teas contained elevated F comparable to the Chinese brick tea.Tea F level is also affected by tea cultivar,soil condition,tea processing method and tea infusion preparation method.
文摘为挖掘乌龙茶品种鲜叶的特征特性,以绿茶品种‘福鼎大毫茶’一芽二叶或三叶为对照(CK),分析比较了高香优质乌龙茶品种‘茗科1号’一芽二叶或三叶(TM)与中小开面二至四叶(MM)在转录和代谢水平的组成差异。结果表明,茶树品种特性和采摘标准为影响茶鲜叶转录和代谢轮廓的重要因素,其中CK、TM和MM的转录与代谢轮廓相互间均有良好的模式区分。差异表达基因与差异代谢物的富集和关联分析结果表明,TM vs CK与MM vs TM的差异表达基因在分子功能、细胞组分和生物过程方面存在不同的GO功能富集模式,且前者拥有较多的生物过程和较少的细胞组分;“植物次生代谢产物的生物合成”、“精氨酸和脯氨酸代谢”与“类胡萝卜素生物合成”、“苯丙素的生物合成”、“甘氨酸、丝氨酸和苏氨酸代谢”分别是TM vsCK、MM vs TM在2种组学分析中的前20条共有KEGG富集代谢通路。TM vs CK中L-苯丙氨酸、反式肉桂酸和4-胍基丁酸丰度的显著降低,以及MM vs TM中胆碱丰度的显著提高和L-苯丙氨酸、L-色氨酸、L-高丝氨酸丰度的显著降低均与相应共富集代谢通路中多个基因的显著上调或下调表达密切相关。此外,MM vs TM中脱落酸丰度的显著提高与“类胡萝卜素生物合成”通路中紫黄质脱环氧化酶和番茄红素β-环化酶基因的显著上调表达高度相关。这可为阐明‘茗科1号’茶鲜叶生物学特征及其多茶类兼制特性提供参考依据。