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A review of the experimental and numerical studies on the compression behavior of the additively produced metallic lattice structures at high and low strain rates
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作者 Muhammad Arslan Bin Riaz Mustafa Guden 《Defence Technology(防务技术)》 2025年第7期1-49,共49页
Recent advances in additive manufacturing have enabled the construction of metallic lattice structures with tailored mechanical and functional properties.One potential application of metallic lattice struc-tures is in... Recent advances in additive manufacturing have enabled the construction of metallic lattice structures with tailored mechanical and functional properties.One potential application of metallic lattice struc-tures is in the impact load mitigation where an external kinetic energy is absorbed by the deformation/crushing of lattice cells.This has motivated a growing number of experimental and numerical studies,recently,on the crushing behavior of additively produced lattice structures.The present study overviews the dynamic and quasi-static crushing behavior of additively produced Ti64,316L,and AlSiMg alloy lattice structures.The first part of the study summarizes the main features of two most commonly used additive processing techniques for lattice structures,namely selective-laser-melt(SLM)and electro-beam-melt(EBM),along with a description of commonly observed process induced defects.In the second part,the deformation and strain rate sensitivities of the selected alloy lattices are outlined together with the most widely used dynamic test methods,followed by a part on the observed micro-structures of the SLM and EBM-processed Ti64,316L and AlSiMg alloys.Finally,the experimental and numerical studies on the quasi-static and dynamic compression behavior of the additively processed Ti64,316L,and AlSiMg alloy lattices are reviewed.The results of the experimental and numerical studies of the dynamic properties of various types of lattices,including graded,non-uniform strut size,hollow,non-uniform cell size,and bio-inspired,were tabulated together with the used dynamic testing methods.The dynamic tests have been noted to be mostly conducted in compression Split Hopkinson Pressure Bar(SHPB)or Taylor-and direct-impact tests using the SHPB set-up,in all of which relatively small-size test specimens were tested.The test specimen size effect on the compression behavior of the lattices was further emphasized.It has also been shown that the lattices of Ti64 and AlSiMg alloys are relatively brittle as compared with the lattices of 316L alloy.Finally,the challenges associated with modelling lattice structures were explained and the micro tension tests and multi-scale modeling techniques combining microstructural characteristics with macroscopic lattice dynamics were recommended to improve the accuracy of the numerical simulations of the dynamic compression deformations of metallic lattice structures. 展开更多
关键词 Metallic lattice structures Additive manufacturing strain rate sensitivity MICROSTRUCTURE Dynamic compression High strain rate loading MODELLING
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Improving the fracture strain of graphite materials by in-situ porosity introduction by two-step sintering
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作者 GU Shi-jia CHEN Han-lin +3 位作者 WANG Jun-zhuo LU Xiao-fang WANG Lian-jun JIANG Wan 《新型炭材料(中英文)》 北大核心 2025年第3期703-716,共14页
High-performance graphite materials have important roles in aerospace and nuclear reactor technologies because of their outstanding chemical stability and high-temperature performance.Their traditional production meth... High-performance graphite materials have important roles in aerospace and nuclear reactor technologies because of their outstanding chemical stability and high-temperature performance.Their traditional production method relies on repeated impregnation-carbonization and graphitization,and is plagued by lengthy preparation cycles and high energy consumption.Phase transition-assisted self-pressurized selfsintering technology can rapidly produce high-strength graphite materials,but the fracture strain of the graphite materials produced is poor.To solve this problem,this study used a two-step sintering method to uniformly introduce micro-nano pores into natural graphite-based bulk graphite,achieving improved fracture strain of the samples without reducing their density and mechanical properties.Using natural graphite powder,micron-diamond,and nano-diamond as raw materials,and by precisely controlling the staged pressure release process,the degree of diamond phase transition expansion was effectively regulated.The strain-to-failure of the graphite samples reached 1.2%,a 35%increase compared to samples produced by fullpressure sintering.Meanwhile,their flexural strength exceeded 110 MPa,and their density was over 1.9 g/cm^(3).The process therefore produced both a high strength and a high fracture strain.The interface evolution and toughening mechanism during the two-step sintering process were investigated.It is believed that the micro-nano pores formed have two roles:as stress concentrators they induce yielding by shear and as multi-crack propagation paths they significantly lengthen the crack propagation path.The two-step sintering phase transition strategy introduces pores and provides a new approach for increasing the fracture strain of brittle materials. 展开更多
关键词 High-performance graphite Phase transition control Two-step sintering process Fracture strain IN-SITU
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Abnormal texture and sensitivity to strain rate during hot-tension of Mg alloy sheets
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作者 ZHANG Hong-yang NIE Hui-hui +1 位作者 XU Xiong LIANG Wei 《Journal of Central South University》 2025年第3期991-1007,共17页
The deformation behavior of hot-rolled AZ31 magnesium(Mg)alloy sheet was analyzed when subjected to uniaxial tension along its normal direction at temperatures ranging from 100 to 400℃and strain rates ranging from 0.... The deformation behavior of hot-rolled AZ31 magnesium(Mg)alloy sheet was analyzed when subjected to uniaxial tension along its normal direction at temperatures ranging from 100 to 400℃and strain rates ranging from 0.5 to 100 mm/min.Based on the stress−strain curves and the dynamic material model,the hot processing map was established,which demonstrates that the power dissipation factor(η)is the most sensitive to strain rate at 400℃via absorption of dislocations.At 400℃,sample at 0.5 mm/min possessesηof 0.89 because of its lower kernel average misorientation(KAM)value of 0.51,while sample at 100 mm/min possessesηof 0.46 with a higher KAM value of 1.147.In addition,the flow stress presents a slight decrease of 25.94 MPa at 10 mm/min compared to that at 100 mm/min and 100℃.The reasons are twofold:a special~34°texture component during 100℃-100 mm/min favoring the activation of basal slip,and dynamic recrystallization(DRX)also providing softening effect to some extent by absorbing dislocations.Difference in activation of basal slip among twin laminas during 100℃-100 mm/min results in deformation inhomogeneity within the grains,which generates stress that helps matrix grains tilt to a direction favorable to basal slip,forming the special~34°texture component. 展开更多
关键词 Mg alloy sheet hot deformation TWINNING abnormal texture strain rate
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A new damage constitutive model for rock strain softening based on an improved Logistic function
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作者 GUO Yun-peng LIU Dong-qiao +1 位作者 YANG Sheng-kai LI Jie-yu 《Journal of Central South University》 2025年第8期3070-3094,共25页
This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening ... This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening and damage process.Simultaneously,it established a constitutive model capable of describing the entire process of rock pre-peak compaction and post-peak strain softening deformation,considering the nonlinear effects of the initial compaction stage of rocks,combined with damage mechanics theory and effective medium theory.In addition,this research verified the rationality of the constructed damage constitutive model using results from uniaxial and conventional triaxial compression tests on Miluo granite,yellow sandstone,mudstone,and glutenite.The results indicate that based on the improved Logistic function,the theoretical damage model accurately describes the entire evolution of damage characteristics during rock compression deformation,from maintenance through gradual onset,accelerated development to deceleration and termination,in a simple and unified expression.At the same time,the constructed constitutive model can accurately simulate the stress-strain process of different rock types under uniaxial and conventional triaxial compression,and the theoretical model curve closely aligns with experimental data.Compared to existing constitutive models,the proposed model has significant advantages.The damage model parameters a,r and β have clear physical meanings and interact competitively,where the three parameters collectively determine the shape of the theoretical stress−strain curve. 展开更多
关键词 rock mechanics strain softening improved Logistic function S-shaped model damage evolution constitutive model
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Dynamic instantaneous dissolution of the precipitates in aged Mg-Zn-Zr alloy at high strain rate
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作者 LIU Yue-yang YANG Yang +6 位作者 HU Li-xiang CHEN Yi KE Yu-bin LI Dan WEI Shao-hong XU Wen-lin CHEN Xiang 《Journal of Central South University》 2025年第6期2038-2050,共13页
The commercial ZK 60 magnesium alloy with extruded state experienced aging heat treatment(T 6)was dynamically loaded at strain rate of 3000 s−1 by means of the split Hopkinson pressure bar(SHPB)in this paper.Transmiss... The commercial ZK 60 magnesium alloy with extruded state experienced aging heat treatment(T 6)was dynamically loaded at strain rate of 3000 s−1 by means of the split Hopkinson pressure bar(SHPB)in this paper.Transmission electron microscopy(TEM)observations showed that the precipitatedβ′_(1) phases partially dissolved(spheroidized)with blurred interfaces within 160μs at 3000 s^(−1).The average length and diameter of the rod-shapedβ′_(1) phase particles were 48.5 and 9.8 nm after the T 6 heat treatment;while the average diameter of the sphericalβ′_(1) phases changed to 8.8 nm after loading.The deformedβ′_(1) phase generated larger lattice distortion energy than Mg matrix under high strain rate loading.Therefore,the difference of free energy(the driving force of dissolution)between theβ′_(1) phase and the matrix increased,making the instantaneous dissolution of theβ′_(1) phase thermodynamically feasible.The dissolution(spheroidization)of theβ′_(1) phase particles was kinetically promoted because the diffusion rate of the solute Zn atoms was accelerated by combined actions of adiabatic temperature rise,high density of dislocations(vacancies)and high deviatoric stresses during high strain rate loading.The increase in hardness of ZK 60-T 6 alloy could be attributed to solid solution strengthening,dislocation strengthening and second phase particle strengthening. 展开更多
关键词 dynamic dissolution(spheroidization) THERMODYNAMICS kinetics high strain rate ZK 60-T 6 magnesium alloy
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Experimental studies and failure mechanisms of strain andfault-slip rockburst:A review
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作者 ZHANG Qing-he WEI Chun-xu +3 位作者 YUAN Liang LIANG Zhi-wei YANG Fa-wang WANG Xiao-rui 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第10期3741-3781,共41页
In recent years,rockburst have gained significant attention as a crucial topic in rock engineering.Strain andfault-slip rockburst are two common types that occur frequently and cause substantial damage.The objective o... In recent years,rockburst have gained significant attention as a crucial topic in rock engineering.Strain andfault-slip rockburst are two common types that occur frequently and cause substantial damage.The objective of thisreview is to conduct a comprehensive study on the experiments and failure mechanisms of strain and fault-slip rockburst.Firstly,the article analyzes the evolving trends in experimental research on rockburst in the past decade,highlightingmechanical properties and failure modes as the primary research focuses in understanding rockburst mechanisms.Subsequently,it provides an overview of the experimental techniques and methods employed for studying both types ofrockburst.Then,with a focus on the mechanical properties and failure modes,the article conducts an extensive analysisof the failure mechanisms associated with strain and fault-slip rockburst.By analyzing experimental data and observingthe failure characteristics of samples,it discusses the variations and common features exhibited by these two types ofrockburst under various test conditions.This analysis is of paramount importance in revealing the causes of rockburstformation and development,as well as in predicting rockburst trends and assessing associated risks.Lastly,thelimitations of current rockburst experiments and future research directions are discussed,followed by a comprehensivesummary of the entire article. 展开更多
关键词 strain rockburst fault-slip rockburst experimental techniques experimental methods failure mechanisms
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Effect of Elastic Strains on Adsorption Energies of C,H and O on Transition Metal Oxides
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作者 XIE Tian SONG Erhong 《无机材料学报》 SCIE EI CAS CSCD 北大核心 2024年第11期1292-1302,共11页
Platinum(Pt)-based noble metal catalysts(PGMs)are the most widely used commercial catalysts,but they have the problems of high cost,low reserves,and susceptibility to small-molecule toxicity.Transition metal oxides(TM... Platinum(Pt)-based noble metal catalysts(PGMs)are the most widely used commercial catalysts,but they have the problems of high cost,low reserves,and susceptibility to small-molecule toxicity.Transition metal oxides(TMOs)are regarded as potential substitutes for PGMs because of their stability in oxidizing environments and excellent catalytic performance.In this study,comprehensive investigation into the influence of elastic strains on the adsorption energies of carbon(C),hydrogen(H)and oxygen(O)on TMOs was conducted.Based on density functional theory(DFT)calculations,these effects in both tetragonal structures(PtO_(2),PdO_(2))and hexagonal structures(ZnO,CdO),along with their respective transition metals were systematically explored.It was identified that the optimal adsorption sites on metal oxides pinpointed the top of oxygen or the top of metal atom,while face-centered cubic(FCC)and hexagonal close-packed(HCP)holes were preferred for the transition metals.Furthermore,under the influence of elastic strains,the results demonstrated significant disparities in the adsorption energies of H and O between oxides and transition metals.Despite these differences,the effect of elastic strains on the adsorption energies of C,H and O on TMOs mirrored those on transition metals:adsorption energies increased under compressive strains,indicating weaker adsorption,and decreased under tension strains,indicating stronger adsorption.This behavior was rationalized based on the d-band model for adsorption atop a metallic atom or the p-band model for adsorption atop an oxygen atom.Consequently,elastic strains present a promising avenue for tailoring the catalytic properties of TMOs. 展开更多
关键词 density functional theory adsorption energy elastic strain engineering transition metal oxide CATALYST
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Entire deformational characteristics and strain localization of jointed rock specimen in plane strain compression 被引量:4
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作者 王学滨 《Journal of Central South University of Technology》 EI 2006年第3期300-306,共7页
Shear band (SB), axial, lateral and volumetric strains as well as Poisson’s ratio of anisotropic jointed rock specimen (JRS) were modeled by Fast Lagrangian Analysis of Continua (FLAC). Failure criterion of rock was ... Shear band (SB), axial, lateral and volumetric strains as well as Poisson’s ratio of anisotropic jointed rock specimen (JRS) were modeled by Fast Lagrangian Analysis of Continua (FLAC). Failure criterion of rock was a composited Mohr-Coulomb criterion with tension cut-off. An inclined joint was treated as square elements of ideal plastic material beyond the peak strength. Several FISH functions were written to automatically find the addresses of elements in the joint and to calculate the entire deformational characteristics of plane strain JRS. The results show that for moderate joint inclination (JI), strain is only concentrated into the joint governing the behavior of JRS, leading to ideal plastic responses in axial and lateral directions. For higher JI, the post-peak stress-axial and lateral strain curves become steeper as JI increases owing to the increase of new SB’s length. Lateral expansion and precursor to the unstable failure are the most apparent, resulting in the highest Poisson’s ratio and even negative volumetric strain. For lower JI, the entire post-peak deformational characteristics are independent of JI. The lowest lateral expansion occurs, leading to the lowest Poisson’s ratio and positive volumetric strain all along. The present prediction on anisotropic strength in plane strain compression qualitatively agrees with the results in triaxial tests of rocks. The JI calculated by Jaeger’s formula overestimates that related to the minimum strength. Advantages of the present numerical model over the Jaeger’s model are pointed out. 展开更多
关键词 jointed rock STRESS axial strain lateral strain volumetric strain Poisson's ratio shear band
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Characteristics and mechanisms of strain waves generated in rock by cylindrical explosive charges 被引量:13
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作者 刘科伟 李萧翰 +3 位作者 李夕兵 姚志华 舒宗宪 袁明华 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第11期2951-2957,共7页
A superposing principle, by suitably adding the strain waves from a number of concentrated explosive charges to approximate the waves generated by a cylindrical charge based on the strain wave of a point or small sphe... A superposing principle, by suitably adding the strain waves from a number of concentrated explosive charges to approximate the waves generated by a cylindrical charge based on the strain wave of a point or small spherical explosive charge generated in rock, is used to further study the triggering time of strain gauges installed in radial direction at same distances but different positions surrounding a cylindrical explosive charge in rock. The duration of the first compression phase and peak value of strain wave, and furthermore, their differences are analyzed and some explanations are given. Besides that, the gauge orientation in which the maximum peak value occurs is also discussed. At last, the effect of velocity of detonation(V.O.D.) of a cylindrical explosive charge on the strain waves generated in the surrounding rock is taken as key research and the pattern of peak amplitude of a strain wave varies with the V.O.D. is likely to have been found. 展开更多
关键词 cylindrical explosive charge strain wave concentrated explosive charge elemental strain wave superposing principle velocity of detonation(V O D velocity of propagation(V O P
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Effect of strain hardening and strain softening on welding distortion and residual stress of A7N01-T4 aluminum alloy by simulation analysis 被引量:8
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作者 闫德俊 刘雪松 +2 位作者 李军 杨建国 方洪渊 《Journal of Central South University》 SCIE EI CAS 2010年第4期666-673,共8页
The effect of strain hardening and strain softening behavior of flow stress changing with temperature on welding residual stress, plastic strain and welding distortion of ATN0 1-T4 aluminum alloy was studied by finite... The effect of strain hardening and strain softening behavior of flow stress changing with temperature on welding residual stress, plastic strain and welding distortion of ATN0 1-T4 aluminum alloy was studied by finite simulation method. The simulation results show that the weld seam undergoes strain hardening in the temperature range of 180-250 ℃, however, it exhibits strain softening at temperature above 250 ℃ during welding heating and cooling process. As a result, the strain hardening and strain softening effects counteract each other, introducing slightly influence on the welding residual stress, residual plastic strain and distortion. The welding longitudinal residual stress was determined by ultrasonic stress measurement method for the flat plates of A7N01-T4 aluminum alloy. The simulation results are well accordant with test ones. 展开更多
关键词 strain hardening strain softening plastic strain welding residual stress
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Strain rate effects on sand and its quantitative analysis 被引量:1
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作者 李福林 彭芳乐 +1 位作者 李建中 KONGKITKUL W 《Journal of Central South University》 SCIE EI CAS 2009年第4期658-662,共5页
Strain rate effects on the stress-strain behavior of sand were investigated by performing special plane strain and triaxial compression tests on saturated and air-dried sand specimens. In these tests, the loading stra... Strain rate effects on the stress-strain behavior of sand were investigated by performing special plane strain and triaxial compression tests on saturated and air-dried sand specimens. In these tests, the loading strain rate was changed many times by a factor of up to 1 000 during otherwise monotonous loading at a constant axial strain rate. Test results show that the stress jump upon a stepwise change in the strain rate decays with an increase in the irreversible strain when monotonous loading continues at the changed strain rate and the amount of stress jump is essentially proportional to the instantaneous stress. Based on the amount of these stress jumps, a parameter fl called the rate-sensitivity coefficient is introduced to represent the quantity of the observed viscous properties of sand, which equals 0.021 3 and 0.024 2 respectively for Hostun and Toyoura sands. Further analyses on the results indicate that the effect of the presence of pore water is deemed to be negligible with sand and the fl value is rather independent of loading method, wet condition and confining pressure. 展开更多
关键词 SAND strain rate viscous properties stress-strain relation
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Effect of Strain Rate on Compression Behavior of Vinyl Ester Resin Casting
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作者 XIONG Tao YANG Bin XIONG Jie XU Xian-jian ZHOU Kai MAO Ming-zhong 《材料工程》 EI CAS CSCD 北大核心 2006年第5期63-67,72,共6页
Quasi-static and high strain rate compressive experiments on vinyl ester casting were carried out by means of MTS (Material Test System) and Hopkinson bar. The behaviors of the compressed unstable and fracture of the ... Quasi-static and high strain rate compressive experiments on vinyl ester casting were carried out by means of MTS (Material Test System) and Hopkinson bar. The behaviors of the compressed unstable and fracture of the resin casting at different strain rates were investigated.The results indicate that the response behavior of the resin casting is controlled by different mechanisms at different strain rate, and some mechanical properties of vinyl ester casting are rate-dependent: the casting are destroyed in toughness model under strain rate 3.3×10 -4~6.6×10 -3/s, while the casting are destroyed in brittleness model under strain rate 950~5800/s. The yield stress, yield strain energy density are all increased with the increasing strain rates at quasi-static as well as at high strain rates. What is interesting is that the yield strain decreased with the strain rates increasing at quasi-static while increased at high strain rates. It is considered that the casting occurred forcing high elastic deformation at high strain rates. The damage of the specimens is mainly controlled by axial stress before unstable deformation, while mainly controlled by shear stress after unstable deformation, and then developed to fracture finally. This progress is rate-dependent: the development of the cracks inside the castings increased with the strain rate increasing. 展开更多
关键词 vinyl ester resin QUASI-STATIC high strain rate strain rate response and crack development
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Genetic Diversity and Global Distribution of Citrus tristeza virus (CTV) Strains
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作者 Wu Xiao-yun Cheng Xiao-fei +1 位作者 Luo Lu Wu Xiao-xia 《Journal of Northeast Agricultural University(English Edition)》 CAS 2012年第2期9-18,共10页
Citrus tristeza virus (CTV), the most devastating viral pathogen in citrus, causes tremendous economic losses to citrus industry worldwide. The CTV isolates exhibit variable pathogenicities on their hosts indicating... Citrus tristeza virus (CTV), the most devastating viral pathogen in citrus, causes tremendous economic losses to citrus industry worldwide. The CTV isolates exhibit variable pathogenicities on their hosts indicating a mixed population of the CTV in nature. Several fragments within the CTV genome have been used for studying the genetic diversity of the CTV, however, the best region for rapid the CTV strain differentiation is still absent at present. In present study, a systemic analysis was carried out to evaluate the best region within the CTV genome for rapid CTV strain differentiation. Results of our study showed that the major coat protein (CP) coding region was the best region for this purpose. Using pair-wise distance frequency distribution plot, a reasonable genetic distance cut-off value was set for the CTV CP gene for the CTV strain differentiation. Using this criterion, eight CTV strains, including seven well characterized and a new strain, were successfully differentiated using 537 CTV isolates reported from 38 countries. The global strain distribution pattern was then determined and discussed. Our results also provided a new insight into the evolution and spreading of the virus, as well as the information for developing proper disease management strategy. 展开更多
关键词 Citrus tristeza virus phylogenetic analysis strain differentiation recombination strain distribution pattern EVOLUTION
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Desulfovibrio dechloracetivorans strain SF3三价铁还原酶的定位和性质的研究(英文) 被引量:2
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作者 王佳晨 林洋 孙宝林 《中国科学技术大学学报》 CAS CSCD 北大核心 2009年第4期337-343,共7页
生理和生化实验结果表明Fe(Ⅲ)-EDTA是SF3偏好的一种可溶性三价铁.连二亚硫酸盐还原后的SF3经过可溶性三价铁氧化后,其紫外可见光谱显示c型细胞色素参与了三价铁的还原过程.铁还原酶的定位实验说明87%的铁还原酶活性定位于细胞可溶性组... 生理和生化实验结果表明Fe(Ⅲ)-EDTA是SF3偏好的一种可溶性三价铁.连二亚硫酸盐还原后的SF3经过可溶性三价铁氧化后,其紫外可见光谱显示c型细胞色素参与了三价铁的还原过程.铁还原酶的定位实验说明87%的铁还原酶活性定位于细胞可溶性组分,74%的活性分布于原生质体中,这种分布情况可能与一种和其他细菌不同的铁还原机制有关.SF3的铁还原酶在25℃,pH7.4时显示最高活性,并且在氧气中表现出较强的稳定性. 展开更多
关键词 三价铁还原酶 DESULFOVIBRIO dechloracetivorans strain SF3 定位 性质
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Providencia rettgeri strain HNR菌株羟胺氧化酶超声波法提取的研究 被引量:2
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作者 黄珏 何义亮 +2 位作者 赵彬 Shauna Taylor 张文英 《生物技术通报》 CAS CSCD 2008年第S1期384-388,共5页
为了更有效和便捷的提取菌株脱氮过程中的关键酶,本文利用超声波破碎法对异养脱氮菌Providencia rettgeri strain HNR进行破碎,通过单因素实验及正交试验,确定出最佳破碎条件,便于后续试验。实验结果表明:最佳超声破碎条件为菌液OD_(600... 为了更有效和便捷的提取菌株脱氮过程中的关键酶,本文利用超声波破碎法对异养脱氮菌Providencia rettgeri strain HNR进行破碎,通过单因素实验及正交试验,确定出最佳破碎条件,便于后续试验。实验结果表明:最佳超声破碎条件为菌液OD_(600)为1.996,工作次数为70次,工作/间歇为5/5s功率为400W的组合为最佳工作条件。在此工作条件下破碎后得到的酶粗提液对羟胺有降解,得到羟胺氧化酶。 展开更多
关键词 超声波法 细胞破碎 PROVIDENCIA rettgeri strain HNR
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Synechocystis sp.strain PCC 6803定量PCR模板制备方法的改进 被引量:2
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作者 阳桂丹 张巨源 陈雯莉 《华中农业大学学报》 CAS CSCD 北大核心 2016年第6期44-51,共8页
以Synechocystis sp.strain PCC 6803为实验菌株,设计改良制备定量PCR模板的方法;同时,对Synechocystis sp.strain PCC 6803总RNA的Trizol抽提法进行优化。结果显示,利用改良方法抽提得到的RNA结构更完整;同时,利用改良方法制备的模板... 以Synechocystis sp.strain PCC 6803为实验菌株,设计改良制备定量PCR模板的方法;同时,对Synechocystis sp.strain PCC 6803总RNA的Trizol抽提法进行优化。结果显示,利用改良方法抽提得到的RNA结构更完整;同时,利用改良方法制备的模板可以用于后续定量PCR实验;其中,只有高表达量基因适用于半定量PCR,低表达量基因则需要实时荧光定量PCR检测。 展开更多
关键词 定量PCR Synechocystissp.strain PCC6803 RNA抽提 qPCR模板制备
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Characteristics of present-day active strain field of Chinese mainland 被引量:9
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作者 郭良迁 李延兴 +1 位作者 杨国华 胡新康 《地震学报》 CSCD 北大核心 2008年第6期560-569,共10页
On the basis of the GPS data obtained from repeated measurements carried out in 2004 and 2007,the horizontal principal strain of the Chinese mainland is calculated,which shows that the direction of principal compressi... On the basis of the GPS data obtained from repeated measurements carried out in 2004 and 2007,the horizontal principal strain of the Chinese mainland is calculated,which shows that the direction of principal compressive strain axis of each subplate is basically consistent with the P-axis of focal mechanism solution and the principal compressive stress axis acquired by geological method.It indicates that the crustal tectonic stress field is relatively stable in regions in a long time.The principal compressive stress axes of Qinghai-Tibet and Xinjiang subplates in the western part of Chinese mainland direct to NS and NNE-SSW,which are controlled by the force from the col-lision of the Eurasia Plate and India Plate.The principal compressive strain axes of Heilongjiang and North China subplates in the eastern part direct to ENE-WSW,which shows that they are subject to the force from the collision and underthrust of the Eurasia Plate to the North America and Pacific plates.At the same time,they are also af-fected by the lateral force from Qinghai-Tibet and Xinjiang subplates.The principal compressive strain axis of South China plate is WNW-ESE,which reflects that it is affected by the force from the collision of Philippine Sea Plate and Eurasia Plate and it is also subject to the lateral force from Qinghai-Tibet subplate.It is apparent from the comparison between the principal compressive strain axes in the periods of 2004~2007 and 2001~2004 that the acting directions of principal compressive stress of subplates in both periods are basically consistent.However,there is certain difference between their directional concentrations of principal compressive stress axes.The sur-face strain rates of different tectonic units in both periods indicate that the events predominating by compressive variation decrease,while the events predominating by tensile change increase. 展开更多
关键词 GPS 应变场 压应变轴 应力 构造活动
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不同电子供体对Desulfovibrio dechloracetivorans strain SF3金属还原的影响(英文)
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作者 林洋 王佳晨 +1 位作者 储诚操 孙宝林 《中国科学技术大学学报》 CAS CSCD 北大核心 2009年第4期440-446,共7页
还原脱氯,脱硫细菌Desulfovibrio dechloracetivorans strain SF3可异化还原三价铁,六价铬和三价钴.乙酸盐,乙醇,氢气,丙酮酸盐,乳酸盐和柠檬酸盐可作为还原可溶性三价铁的电子供体,而只有丙酮酸盐和氢气做电子供体时SF3才能还原不可溶... 还原脱氯,脱硫细菌Desulfovibrio dechloracetivorans strain SF3可异化还原三价铁,六价铬和三价钴.乙酸盐,乙醇,氢气,丙酮酸盐,乳酸盐和柠檬酸盐可作为还原可溶性三价铁的电子供体,而只有丙酮酸盐和氢气做电子供体时SF3才能还原不可溶的非晶态三价铁氧化物.实验测定Fe(Ⅲ)-EDTA还原对乙酸盐氧化的比例为7.76±0.35,而且该过程中没有伴随细菌的生长.SF3可利用氢气,丙酮酸盐或乳酸盐作为电子供体还原六价铬和三价钴,但这一过程不能利用乙酸盐作为电子供体.据我们所知,这是首次发现一种SRB(硫酸盐还原菌)可利用乙酸盐还原可溶性三价铁,但无法利用其还原不可溶三价铁以及六价铬和三价钴,该发现说明SF3在金属还原过程中利用了较复杂的电子传递系统. 展开更多
关键词 金属还原 DESULFOVIBRIO dechloracetivorans strain SF3
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Effect of ultraviolet mutagenesis on heterotrophic strain mutation and bioleaching of low grade copper ore 被引量:5
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作者 WU Ai-xiang HU Kai-jian +2 位作者 WANG Hong-jiang ZHANG Ai-qing YANG Ying 《Journal of Central South University》 SCIE EI CAS CSCD 2017年第10期2245-2252,共8页
The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity... The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity of bacteria was improved after ultraviolet mutagenesis; the best irradiation time was 120 s. Compared to the original bacteria, the cells density of mutant bacteria at stationary phase increased by 26% and ammonia produced by mutant bacteria increased by 12%. Higher activity of bacteria leads to a higher copper extraction rate. The bioleaching performance of Providencia JAT-1 was improved after UV mutagenesis. The copper extraction rate with mutant bacteria increased by 10.6% compared to the original bacteria. The ore surface was corroded and the fine particles were absent after bioleaching. Free copper oxide and copper silicates could be leached out easily by using JAT-1; a small part of the copper sulfide can also be leached out. Bioleaching using JAT-1 is more effective than ammonia leaching and copper extraction rate with mutant bacteria was 21.1% higher than that by ammonia leaching under the same condition. 展开更多
关键词 ULTRAVIOLET MUTAGENESIS BIOLEACHING HETEROTROPHIC strain ammonia low grade copper ORE
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Energy consumption in rock fragmentation at intermediate strain rate 被引量:19
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作者 洪亮 周子龙 +2 位作者 尹土兵 廖国燕 叶洲元 《Journal of Central South University》 SCIE EI CAS 2009年第4期677-682,共6页
In order to determine the relationship among energy consumption of rock and its fragmentation, dynamic strength and strain rate, granite, sandstone and limestone specimens were chosen and tested on large-diameter spli... In order to determine the relationship among energy consumption of rock and its fragmentation, dynamic strength and strain rate, granite, sandstone and limestone specimens were chosen and tested on large-diameter split Hopkinson pressure bar (SHPB) equipment with half-sine waveform loading at the strain rates ranging from 40 to 150 s- 1. With recorded signals, the energy consumption, strain rate and dynamic strength were analyzed. And the fragmentation behaviors of specimens were investigated. The experimental results show that the energy consumption density of rock increases linearly with the total incident energy. The energy consumption density is of an exponent relationship with the average size of rock fragments. The higher the energy consumption density, the more serious the fragmentation, and the better the gradation of fragments. The energy consumption density takes a good logarithm relationship with the dynamic strength of rock. The dynamic strength of rock increases with the increase of strain rate, indicating higher strain rate sensitivity. 展开更多
关键词 ROCK large-diameter SHPB test intermediate strain rate energy consumption density fragment distribution dynamic strength
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