Endoscop c manometry of sphincter of Oddi(SO)and serumlevels of gastrin,glucagon,and somatostatin were measured in patients withpostcholecystectomy syndrome(n=12),asymptomatic cholecystectomised pa-tients(n=6),and con...Endoscop c manometry of sphincter of Oddi(SO)and serumlevels of gastrin,glucagon,and somatostatin were measured in patients withpostcholecystectomy syndrome(n=12),asymptomatic cholecystectomised pa-tients(n=6),and controlled subjects(n=14).Pentagastrin-stimulated gastricacid secretion test was also performed in part of patients who had symptoms orno symptoms after gallbladder resection.The results showed that the patientsof symptomatic group had hypertonic dyskinesia of SO as shown by deep andwide waves superimposed on high basal pressure plateau of SO.The symptoma-tic group also had a higher serum level of gastrin and a greater BAO than tho-se of other two groups.No difference of serum levels of glucagon and soma-tostatin was found among these three groups.The hypertonic dyskinesia of SOand hypergastrinemia are considered as possibly important factors in the patho-genesis of postcholecystectomy syndrome.展开更多
Objective: To discuss the relationship between hypercholesterolemic disease and the functional and structural changes of Sphincter of Oddi (SO) by the study of effect of Cholesterol Liposome (CL) on structural and qua...Objective: To discuss the relationship between hypercholesterolemic disease and the functional and structural changes of Sphincter of Oddi (SO) by the study of effect of Cholesterol Liposome (CL) on structural and quantitative changes of SO cells. Methods: Rabbit SO was isolated for primary cell culture and subculture. After subcultured with different concentration of CL culture medium for 20 h, the structural and quantitative changes of SO cells were analyzed and detected by MTT-test, flow cytometer (FCM), electronic microscope and electrophoresis technique respectively. Results: CL contributed a prominent stimulus to SO cells proliferation at middle concentration (<0. 5 - 0. 8 mg/ml), which could be confirmed by FCM analysis which indicated the number of SO cells in S-phase increasing remarkably; however, high concentration of CL inhibited SO cells' proliferation (>1. 0 mg/ml) and induced apoptosis of SO cells. Swelled mitochondria and dilated endoplasmic reticulum as well as disjoined and diminished microfilaments were found in SO cells by electronic microscopy. The content of SO cells actin decreased with the increment of cholesterol concentration. There was a significant difference of actin content between CL groups and control group (P<0. 05). Conclusion: CL may change SO cell membrane's function, organelle's structure and especially the quantity and configuration of microfilaments, at the same time, CL at different concentration can induce changes of SO cells cycle and lead to different changes in the number of SO cells.展开更多
目的系统评价和整合直肠癌保肛术患者肠道症状的真实体验,为更好地管理肠道症状提供参考。方法计算机检索中国知网、万方、维普、CBM、PubMed、EMbase、Web of Science、Science Direct、CINAHL数据库中关于直肠癌保肛术患者肠道症状体...目的系统评价和整合直肠癌保肛术患者肠道症状的真实体验,为更好地管理肠道症状提供参考。方法计算机检索中国知网、万方、维普、CBM、PubMed、EMbase、Web of Science、Science Direct、CINAHL数据库中关于直肠癌保肛术患者肠道症状体验的质性研究,检索时限为建库至2023年10月23日。使用英国牛津大学循证医学中心文献质量评价项目评价纳入的文献,采用汇集性整合方法对结果进行分析。结果共纳入14篇文献,提炼出44个研究结果,将相似的结果归纳为10个新类别,整合成3个结果:保肛术患者肠道症状体验差;保肛术患者采取应对肠道症状的策略;保肛术患者面对肠道症状存在的信息和情感需求突出。结论直肠癌保肛术后的肠道症状给患者带来较大的身心困扰,在与症状共存过程中患者通过观察与自我调节,采取不同的应对策略,不断积累自我管理经验,但仍感到知识及情感方面的支持不足,医护人员应加强对直肠癌患者术后肠道症状的重视,针对患者自我管理中的薄弱环节,进一步完善干预策略,为患者提供支持性护理,提高其自我管理能力。展开更多
文摘Endoscop c manometry of sphincter of Oddi(SO)and serumlevels of gastrin,glucagon,and somatostatin were measured in patients withpostcholecystectomy syndrome(n=12),asymptomatic cholecystectomised pa-tients(n=6),and controlled subjects(n=14).Pentagastrin-stimulated gastricacid secretion test was also performed in part of patients who had symptoms orno symptoms after gallbladder resection.The results showed that the patientsof symptomatic group had hypertonic dyskinesia of SO as shown by deep andwide waves superimposed on high basal pressure plateau of SO.The symptoma-tic group also had a higher serum level of gastrin and a greater BAO than tho-se of other two groups.No difference of serum levels of glucagon and soma-tostatin was found among these three groups.The hypertonic dyskinesia of SOand hypergastrinemia are considered as possibly important factors in the patho-genesis of postcholecystectomy syndrome.
文摘Objective: To discuss the relationship between hypercholesterolemic disease and the functional and structural changes of Sphincter of Oddi (SO) by the study of effect of Cholesterol Liposome (CL) on structural and quantitative changes of SO cells. Methods: Rabbit SO was isolated for primary cell culture and subculture. After subcultured with different concentration of CL culture medium for 20 h, the structural and quantitative changes of SO cells were analyzed and detected by MTT-test, flow cytometer (FCM), electronic microscope and electrophoresis technique respectively. Results: CL contributed a prominent stimulus to SO cells proliferation at middle concentration (<0. 5 - 0. 8 mg/ml), which could be confirmed by FCM analysis which indicated the number of SO cells in S-phase increasing remarkably; however, high concentration of CL inhibited SO cells' proliferation (>1. 0 mg/ml) and induced apoptosis of SO cells. Swelled mitochondria and dilated endoplasmic reticulum as well as disjoined and diminished microfilaments were found in SO cells by electronic microscopy. The content of SO cells actin decreased with the increment of cholesterol concentration. There was a significant difference of actin content between CL groups and control group (P<0. 05). Conclusion: CL may change SO cell membrane's function, organelle's structure and especially the quantity and configuration of microfilaments, at the same time, CL at different concentration can induce changes of SO cells cycle and lead to different changes in the number of SO cells.
文摘目的系统评价和整合直肠癌保肛术患者肠道症状的真实体验,为更好地管理肠道症状提供参考。方法计算机检索中国知网、万方、维普、CBM、PubMed、EMbase、Web of Science、Science Direct、CINAHL数据库中关于直肠癌保肛术患者肠道症状体验的质性研究,检索时限为建库至2023年10月23日。使用英国牛津大学循证医学中心文献质量评价项目评价纳入的文献,采用汇集性整合方法对结果进行分析。结果共纳入14篇文献,提炼出44个研究结果,将相似的结果归纳为10个新类别,整合成3个结果:保肛术患者肠道症状体验差;保肛术患者采取应对肠道症状的策略;保肛术患者面对肠道症状存在的信息和情感需求突出。结论直肠癌保肛术后的肠道症状给患者带来较大的身心困扰,在与症状共存过程中患者通过观察与自我调节,采取不同的应对策略,不断积累自我管理经验,但仍感到知识及情感方面的支持不足,医护人员应加强对直肠癌患者术后肠道症状的重视,针对患者自我管理中的薄弱环节,进一步完善干预策略,为患者提供支持性护理,提高其自我管理能力。
