Physiological studies of soybean [Glycine max(L.)Merr.]genotypes with wide differences in seed protein concentration may permit detection of important yield related processes.In order to research the law of protein a...Physiological studies of soybean [Glycine max(L.)Merr.]genotypes with wide differences in seed protein concentration may permit detection of important yield related processes.In order to research the law of protein accumulation and the characteristics of N accumulation and translocation,we did an experiment with three soybean cultivars which have different protein content and the similar phase in pot culture.The results showed that the laws of protein accumulation of three soybean cultivars are similar in the course of seeding;protein content descended in the early stage,and increased steadily in the middle period,then increased quickly in the later period.But the speed of protein accumulation in soybean seeds was difference in different period.In addition,high protein cultivar (Dongnong 42) and intermediate protein cultivar (Dongnong 7819) were more than those of low protein cultivar (Dongnong 434),including nitrogen contents in leaf and petiole,stem and pod,peak value of nitrogen accumulation of the whole plant,value of nitrogen translocation,its efficiency.展开更多
Three different genotypes soybeans were adopted in this experiment under three fertilizer levels. The object of this study was to investigate protein accumulation regulation of soybean cultivars under the condition of...Three different genotypes soybeans were adopted in this experiment under three fertilizer levels. The object of this study was to investigate protein accumulation regulation of soybean cultivars under the condition of different nutrient levels, and their effects on soybean yield and quality, and to provide theoretical evidence for breed, cultivation and agricultural production, also man-powered controllable locations. The concentration of N in the leaves declined after seedling stage, then increased again at stage of early flowering, and started to decrease up to leaf senescence, declined rapidly from seed-filling season to stage of yellow ripeness. The concentration of N in the stems and pod walls declined with growth stage. High seed protein genotypes exhibited higher N assimilating and partitioning during whole growth stages. Pod walls were media of N partitioning. Protein was accumulated mainly during the later period of reproductive growth stage up to harvest, so plant growth after stage of yellow ripeness could not be neglected.展开更多
Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduce...Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduced plant stature and deficiency in various 7S and 11S subunits,designated as snd1 mutants.Under normal growth conditions,the snd1 mutants showed a severe dwarf phenotype,with plant height of about 25 cm.Compared with wild-type DN47,the mutant snd1 exhibited no obvious morphological differences at the early stage of development.All the snd1 mutants examined had fewer nodes and shorter than normal internodes;the leaves were similar in shape to normal parents,but were dark-green at the mature stage.The flower size was similar to DN47;however,the flowering period was shorter than in the wild-type.Significant variation was noted for protein content,oil content of the seeds and size of seeds(weight of 100 seeds)among 17 snd1 dwarf lines.Genetic analysis indicated that the dwarfism of snd1 was controlled by a single recessive gene.The snd1 dwarf mutant had markedly different dynamic levels of the endogenous hormones gibberellin(GA),brassinosteroid,indole-3-acetic acid and abscisic acid,at the seedling stage.Exogenous GA3 treatment led to recovery of the plant height phenotype of the snd1 mutant;GA3 at 0.1 mm had the largest effect on enhancing plant height.Using molecular markers,snd1 gene was approximately mapped in an interval of 603 kb between markers Satt166 and Satt561 on chromosome 19.Snd1 mutant provided valuable material for hypoallergenic soybean breeding and the snd1 gene might be a novel gene related to plant height in soybean.展开更多
A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference ...A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.展开更多
Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explore...Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explored.In this study,the impact of simulated GI digestion and transepithelial transport on various antioxidant properties of SPH were investigated.SPH displayed DPPH radical scavenging(IC50=4.22 mg/m L),ABTS·+radical scavenging(IC50=2.93 mg/m L),reducing power and metal ion-chelating activities(IC50=0.67 mg/m L).Furthermore,SPH significantly(P<0.05)inhibited the generation of intracellular reactive oxygen species(ROS)in Caco-2 cells.After simulated GI digestion,the antioxidant properties of SPH were enhanced,except for a decrease in ABTS·+radical scavenging activity.After transepithelial transport,the permeates maintained partial antioxidant activity and the LC-MS/MS data further identified the absorbed soybean peptides.These results suggest that SPH contains the antioxidant peptides that are potentially bioavailable and can be regarded as a promising source of functional food ingredients.展开更多
Soybeans are known as a promising source of bioactive peptides.However,knowledge on the antioxidant behaviors of soybean protein hydrolysate(SPH)in the human intestinal epithelium is limited.In this study,SPH was prep...Soybeans are known as a promising source of bioactive peptides.However,knowledge on the antioxidant behaviors of soybean protein hydrolysate(SPH)in the human intestinal epithelium is limited.In this study,SPH was prepared with Alcalase and subsequently ultrafiltered into four peptide fractions as SPH-I(<3 kDa),SPH-II(3~5 k Da),SPH-III(5~10 k Da)and SPH-IV(>10 kDa).The antioxidant properties of SPH and membrane fractions were investigated using different chemical assays and their protective effects against oxidative stress were evaluated using H2 O2-stressed human intestinal Caco-2 cells.Results showed that SPH-I exhibited the strongest 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging activity(IC50=2.56 mg/m L)and reducing capacity while SPH-III had the best metal ion-chelating activity(IC50=0.29 mg/m L).Both SPH and the peptide fractions dose-dependently suppressed intracellular reactive oxygen species(ROS)accumulation induced by H2O2 in Caco-2 cells,but the strongest inhibitory effect was observed for SPH-I.Amino acid(AA)results revealed that SPH-I was rich in hydrophobic and antioxidant AAs,which could contribute to its stronger antioxidant properties.Additionally,SPH-I protected Caco-2 cells from H2O2-induced oxidative stress via inhibiting lipid peroxidation and stimulating antioxidant enzyme activities.These results suggest that SPH-I and constitutive peptides can be beneficial ingredients with antioxidant properties and protective effects against ROS-mediated intestinal injury.展开更多
Genetic characters of 10 soybean breeds in Heilongjiang Province were studied using RAPD technique. We calculated the genetic similarity and genetic distance, made the cluster analysis, and also studied the genetic re...Genetic characters of 10 soybean breeds in Heilongjiang Province were studied using RAPD technique. We calculated the genetic similarity and genetic distance, made the cluster analysis, and also studied the genetic relationship among the 10 breeds. We found 93 amplification segments by RAPE） analysis, in which there were 70 polymorphism segments, 75.26% of amplification segments, using 13 random primers, 11, 12, 5, 4, 7, 8, 3, 7, 2, 5, 10, 10, 9 amplificated bands were found. There were 7.15 bands per primer in average, The genetic similarity among the breeds ranged from 0.6848 to 0.9019, and the genetic distance ranged from 0.0981 to 0.3152. Genetic relationship between 90035 and 9674 was the closest, and between Heifeng 27 and 90035 was the farthest. Protein analysis revealed that the closest genetic relationship was between 90035 and 90024, and the farthest was between 1330 and Sui 4, and between 1330 and Heinong 39.展开更多
Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease c...Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.展开更多
大豆分离蛋白(Soy Protein Isolate,SPI)功能特性决定应用领域,加工过程中NaOH添加量影响SPI功能特性。文章究利用Matlab模拟,采用传统比例积分微分(proportional integral derivative,PID)和模糊自适应控制(Adaptive Fuzzy Control,AFC...大豆分离蛋白(Soy Protein Isolate,SPI)功能特性决定应用领域,加工过程中NaOH添加量影响SPI功能特性。文章究利用Matlab模拟,采用传统比例积分微分(proportional integral derivative,PID)和模糊自适应控制(Adaptive Fuzzy Control,AFC)方法调控NaOH,中和大豆凝乳,并分析其对SPI结构和功能特性的影响。结果表明,pH值为7时,常规PID控制方法NaOH超调量为1.86%,调节时间为47.1 s;模糊自适应控制方法无超调量,调节时间为35.8 s。模糊自适应控制下,SPI傅里叶红外光谱吸收峰更稳定,紫外吸收特性更佳,二级结构向有序转变,表面疏水性较高,且溶解性、起泡性、泡沫稳定性、乳化性和乳化稳定性均优于常规PID控制方法。展开更多
The expression of protein and oil content of soybean seeds in worldwide was studied. The results shown that: Latitude and year's weather conditions affected protein content of soybean seeds importantly. Elevation ...The expression of protein and oil content of soybean seeds in worldwide was studied. The results shown that: Latitude and year's weather conditions affected protein content of soybean seeds importantly. Elevation affected oil content significantly. There was important difference in protein / oil content among different eco - geographical regions. The zone, in which there was the highest content of protein / oil, was that one with latitude from 0°to 20°59′ . And in this zone, there were different elevation regions, in which protein or oil content was the highest. For high pro-tein, it was the elevation region with 500 - 1000 m; for oil, it was 0~ 500 m. Protein / oil content was also different among different years. However, the changing range among years in Tropical and Subtropical regions was smaller than that in Temperate Regions. The relationship between protein / oil content and latitude, elevation or soil pH in the regions outside the Tropic of concerned and the Tropic of Capricorn was more significant than that in the region inside Regression Lines, i. e. Equatorial Rainforest and Tropical Grassland regions.展开更多
基金Research wassubsidized by thegrandtechnology- surm ounted research items of the National Science Committee ofthe Tenth five- year plan:the research and demonstration of the technology for good- quality soybean
文摘Physiological studies of soybean [Glycine max(L.)Merr.]genotypes with wide differences in seed protein concentration may permit detection of important yield related processes.In order to research the law of protein accumulation and the characteristics of N accumulation and translocation,we did an experiment with three soybean cultivars which have different protein content and the similar phase in pot culture.The results showed that the laws of protein accumulation of three soybean cultivars are similar in the course of seeding;protein content descended in the early stage,and increased steadily in the middle period,then increased quickly in the later period.But the speed of protein accumulation in soybean seeds was difference in different period.In addition,high protein cultivar (Dongnong 42) and intermediate protein cultivar (Dongnong 7819) were more than those of low protein cultivar (Dongnong 434),including nitrogen contents in leaf and petiole,stem and pod,peak value of nitrogen accumulation of the whole plant,value of nitrogen translocation,its efficiency.
基金Natural Science Foundation of Heilongjiang Province (IJN04-0403)
文摘Three different genotypes soybeans were adopted in this experiment under three fertilizer levels. The object of this study was to investigate protein accumulation regulation of soybean cultivars under the condition of different nutrient levels, and their effects on soybean yield and quality, and to provide theoretical evidence for breed, cultivation and agricultural production, also man-powered controllable locations. The concentration of N in the leaves declined after seedling stage, then increased again at stage of early flowering, and started to decrease up to leaf senescence, declined rapidly from seed-filling season to stage of yellow ripeness. The concentration of N in the stems and pod walls declined with growth stage. High seed protein genotypes exhibited higher N assimilating and partitioning during whole growth stages. Pod walls were media of N partitioning. Protein was accumulated mainly during the later period of reproductive growth stage up to harvest, so plant growth after stage of yellow ripeness could not be neglected.
基金Supported by the Ministry of Science and Technology of China(2016YFD0100500)Funding from Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)+4 种基金the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)National Natural Science Foundation of China(31801386)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-2018158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)
文摘Dwarfing is useful to reduce plant height,when breeding high-yielding and non-lodging crops.In this study,a set of natural storage protein subunit-null dwarf mutants of soybean was reported that showed strongly reduced plant stature and deficiency in various 7S and 11S subunits,designated as snd1 mutants.Under normal growth conditions,the snd1 mutants showed a severe dwarf phenotype,with plant height of about 25 cm.Compared with wild-type DN47,the mutant snd1 exhibited no obvious morphological differences at the early stage of development.All the snd1 mutants examined had fewer nodes and shorter than normal internodes;the leaves were similar in shape to normal parents,but were dark-green at the mature stage.The flower size was similar to DN47;however,the flowering period was shorter than in the wild-type.Significant variation was noted for protein content,oil content of the seeds and size of seeds(weight of 100 seeds)among 17 snd1 dwarf lines.Genetic analysis indicated that the dwarfism of snd1 was controlled by a single recessive gene.The snd1 dwarf mutant had markedly different dynamic levels of the endogenous hormones gibberellin(GA),brassinosteroid,indole-3-acetic acid and abscisic acid,at the seedling stage.Exogenous GA3 treatment led to recovery of the plant height phenotype of the snd1 mutant;GA3 at 0.1 mm had the largest effect on enhancing plant height.Using molecular markers,snd1 gene was approximately mapped in an interval of 603 kb between markers Satt166 and Satt561 on chromosome 19.Snd1 mutant provided valuable material for hypoallergenic soybean breeding and the snd1 gene might be a novel gene related to plant height in soybean.
基金Supported by Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)+3 种基金the National Natural Science Foundation of China(31801386)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-2018158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)
文摘A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.
基金the financial support received from National Natural Science Foundation of China(No.31430067 and 31601475)China Postdoctoral Science Foundation funded project(No.2017M610200)Postdoctoral Foundation of Heilongjiang Province(No.LBH-Z17011)
文摘Peptides from Alcalase-hydrolyzed soybean protein hydrolysate(SPH)may hold the potential as natural antioxidants.In addition,the effect of human gastrointestinal(GI)tract on peptide bioavailability needs to be explored.In this study,the impact of simulated GI digestion and transepithelial transport on various antioxidant properties of SPH were investigated.SPH displayed DPPH radical scavenging(IC50=4.22 mg/m L),ABTS·+radical scavenging(IC50=2.93 mg/m L),reducing power and metal ion-chelating activities(IC50=0.67 mg/m L).Furthermore,SPH significantly(P<0.05)inhibited the generation of intracellular reactive oxygen species(ROS)in Caco-2 cells.After simulated GI digestion,the antioxidant properties of SPH were enhanced,except for a decrease in ABTS·+radical scavenging activity.After transepithelial transport,the permeates maintained partial antioxidant activity and the LC-MS/MS data further identified the absorbed soybean peptides.These results suggest that SPH contains the antioxidant peptides that are potentially bioavailable and can be regarded as a promising source of functional food ingredients.
基金financially supported by the National Natural Science Foundation of China(No.31430067,31601475,31571876,and 31671807)the 13th Five-Year Plan(No.2016YFD0401402)
文摘Soybeans are known as a promising source of bioactive peptides.However,knowledge on the antioxidant behaviors of soybean protein hydrolysate(SPH)in the human intestinal epithelium is limited.In this study,SPH was prepared with Alcalase and subsequently ultrafiltered into four peptide fractions as SPH-I(<3 kDa),SPH-II(3~5 k Da),SPH-III(5~10 k Da)and SPH-IV(>10 kDa).The antioxidant properties of SPH and membrane fractions were investigated using different chemical assays and their protective effects against oxidative stress were evaluated using H2 O2-stressed human intestinal Caco-2 cells.Results showed that SPH-I exhibited the strongest 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical scavenging activity(IC50=2.56 mg/m L)and reducing capacity while SPH-III had the best metal ion-chelating activity(IC50=0.29 mg/m L).Both SPH and the peptide fractions dose-dependently suppressed intracellular reactive oxygen species(ROS)accumulation induced by H2O2 in Caco-2 cells,but the strongest inhibitory effect was observed for SPH-I.Amino acid(AA)results revealed that SPH-I was rich in hydrophobic and antioxidant AAs,which could contribute to its stronger antioxidant properties.Additionally,SPH-I protected Caco-2 cells from H2O2-induced oxidative stress via inhibiting lipid peroxidation and stimulating antioxidant enzyme activities.These results suggest that SPH-I and constitutive peptides can be beneficial ingredients with antioxidant properties and protective effects against ROS-mediated intestinal injury.
文摘Genetic characters of 10 soybean breeds in Heilongjiang Province were studied using RAPD technique. We calculated the genetic similarity and genetic distance, made the cluster analysis, and also studied the genetic relationship among the 10 breeds. We found 93 amplification segments by RAPE） analysis, in which there were 70 polymorphism segments, 75.26% of amplification segments, using 13 random primers, 11, 12, 5, 4, 7, 8, 3, 7, 2, 5, 10, 10, 9 amplificated bands were found. There were 7.15 bands per primer in average, The genetic similarity among the breeds ranged from 0.6848 to 0.9019, and the genetic distance ranged from 0.0981 to 0.3152. Genetic relationship between 90035 and 9674 was the closest, and between Heifeng 27 and 90035 was the farthest. Protein analysis revealed that the closest genetic relationship was between 90035 and 90024, and the farthest was between 1330 and Sui 4, and between 1330 and Heinong 39.
基金Supported by the National Items of Research and Industrial Development of Transgenic Plants(J99-B-013)
文摘Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.
文摘大豆分离蛋白(Soy Protein Isolate,SPI)功能特性决定应用领域,加工过程中NaOH添加量影响SPI功能特性。文章究利用Matlab模拟,采用传统比例积分微分(proportional integral derivative,PID)和模糊自适应控制(Adaptive Fuzzy Control,AFC)方法调控NaOH,中和大豆凝乳,并分析其对SPI结构和功能特性的影响。结果表明,pH值为7时,常规PID控制方法NaOH超调量为1.86%,调节时间为47.1 s;模糊自适应控制方法无超调量,调节时间为35.8 s。模糊自适应控制下,SPI傅里叶红外光谱吸收峰更稳定,紫外吸收特性更佳,二级结构向有序转变,表面疏水性较高,且溶解性、起泡性、泡沫稳定性、乳化性和乳化稳定性均优于常规PID控制方法。
文摘The expression of protein and oil content of soybean seeds in worldwide was studied. The results shown that: Latitude and year's weather conditions affected protein content of soybean seeds importantly. Elevation affected oil content significantly. There was important difference in protein / oil content among different eco - geographical regions. The zone, in which there was the highest content of protein / oil, was that one with latitude from 0°to 20°59′ . And in this zone, there were different elevation regions, in which protein or oil content was the highest. For high pro-tein, it was the elevation region with 500 - 1000 m; for oil, it was 0~ 500 m. Protein / oil content was also different among different years. However, the changing range among years in Tropical and Subtropical regions was smaller than that in Temperate Regions. The relationship between protein / oil content and latitude, elevation or soil pH in the regions outside the Tropic of concerned and the Tropic of Capricorn was more significant than that in the region inside Regression Lines, i. e. Equatorial Rainforest and Tropical Grassland regions.
