Spiders spin multiple types of silks that are renowned for their superb mechanical properties.Cylindrical silk with novel molecular and mechanical properties,used in the construction of egg case outercover,is only one...Spiders spin multiple types of silks that are renowned for their superb mechanical properties.Cylindrical silk with novel molecular and mechanical properties,used in the construction of egg case outercover,is only one type of spider silk full-length cDNA reported data to date.In order to provide some valuable messages for large-scale mimicking the outstanding high-performance properties of native spider silk by bioengineering method in the future,two different sizes of cylindrical silk protein genes(full-length and partial cDNA sequences)and EGFP fusion genes were expressed in insect cells under the control of strong promoter by Bacto-Bac/AcNPV baculovirus expression system.EGFP fluorescence and western blotting analysis showed two different sizes of the expressed egg case silk fusion protein presented obviously different solubility within the aqueous cytosol of insect cells.The expression products of partial egg case silk fusion gene were soluble in aqueous cytosol while another larger fusion protein containing almost full-length egg case silk protein tends to form aggregate by self assemblage.Our results also suggested that the molecular size of spider silk protein could be important to assemble them into high-performance silk fiber.展开更多
Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together w...Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together with tissue engineering is increasingly becoming a potential choice of treatment.However,direct transplantation of stem cells without scaffolds has yielded poor clinical outcome.Here we show a strategy of using mouse embryonic stem cells(ESCs)cultured within a silk fibroin(SF)based,three-dimensional scaffold with oriented channels by a directional temperature field freezing technique and lysophilization.We find that the ESCs maintained proliferation and migrated in the scaffolds and the cells migrated fastest along the SF channels.SF scaffolds contributed to ESC differentiation into neural and glial cell like cells and expressions of the neural and glial cell markers MAP2 and GFAP were greatly elevated when retinoic acid was used as an inducing factor.Our results suggest that this approach may offer some hope in the future for spinal cord injury repair using SF scaffolds and ESCs.展开更多
Based on the rapid promotion of"Maritime Silk Road"and the proposal of the Belt and Road initiative by China,this empirical study established the initiative of"Maritime Silk Road"economic entity th...Based on the rapid promotion of"Maritime Silk Road"and the proposal of the Belt and Road initiative by China,this empirical study established the initiative of"Maritime Silk Road"economic entity through the participation of small and medium enterprises in trades along the maritime silk road.The study filled the gap of research on maritime economy in China,put forward the"maritime economy"priority development initiative,and thus discovered whether it's feasible to promote regional integration-the inspiration of maritime economy;it is of great significance in the economic sense to find out that how to face the interference of trade related to leading powers,and the inspiration of the manufacturing industry for maritime economy along the Belt and Road.展开更多
For Xi'an,the central city of the Silk Road Economic Belt,it is both the theoretical and practical problem to develop into an international metropolis,so how to develop into an international metropolis becomes the...For Xi'an,the central city of the Silk Road Economic Belt,it is both the theoretical and practical problem to develop into an international metropolis,so how to develop into an international metropolis becomes the research focus. This paper firstly introduces the necessity,the current situation and the evaluation index system of the construction of the city Xi'an in the context of the Silk Road Economic Belt; then it analyzes the econom ic developm ent of the three old districts,the three new districts,the three suburban districts and the four counties; and finally it puts forward the development path for Xi'an in the context of the Silk Road Economic Belt — the development pattern of "five districts,one international harbor area and two bases""nine-grid-pattern "economic development and the function-oriented coordinated development of the regional economy.展开更多
Translation,as a medium of globalization and the means of overcoming the Babylonian curse,is approached differently by varied translation theories,among which the Skopos approach seems to the one that guides the trans...Translation,as a medium of globalization and the means of overcoming the Babylonian curse,is approached differently by varied translation theories,among which the Skopos approach seems to the one that guides the translation of the nomination dossier in an implicit manner.The dossier,distinct from other genres of texts,has a very clear function which explains why the Skopos theory plays an invisible hand throughout its translation.展开更多
文摘Spiders spin multiple types of silks that are renowned for their superb mechanical properties.Cylindrical silk with novel molecular and mechanical properties,used in the construction of egg case outercover,is only one type of spider silk full-length cDNA reported data to date.In order to provide some valuable messages for large-scale mimicking the outstanding high-performance properties of native spider silk by bioengineering method in the future,two different sizes of cylindrical silk protein genes(full-length and partial cDNA sequences)and EGFP fusion genes were expressed in insect cells under the control of strong promoter by Bacto-Bac/AcNPV baculovirus expression system.EGFP fluorescence and western blotting analysis showed two different sizes of the expressed egg case silk fusion protein presented obviously different solubility within the aqueous cytosol of insect cells.The expression products of partial egg case silk fusion gene were soluble in aqueous cytosol while another larger fusion protein containing almost full-length egg case silk protein tends to form aggregate by self assemblage.Our results also suggested that the molecular size of spider silk protein could be important to assemble them into high-performance silk fiber.
基金supported by funds from Huazhong University of Science and Technology,Wuhan,China
文摘Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together with tissue engineering is increasingly becoming a potential choice of treatment.However,direct transplantation of stem cells without scaffolds has yielded poor clinical outcome.Here we show a strategy of using mouse embryonic stem cells(ESCs)cultured within a silk fibroin(SF)based,three-dimensional scaffold with oriented channels by a directional temperature field freezing technique and lysophilization.We find that the ESCs maintained proliferation and migrated in the scaffolds and the cells migrated fastest along the SF channels.SF scaffolds contributed to ESC differentiation into neural and glial cell like cells and expressions of the neural and glial cell markers MAP2 and GFAP were greatly elevated when retinoic acid was used as an inducing factor.Our results suggest that this approach may offer some hope in the future for spinal cord injury repair using SF scaffolds and ESCs.
文摘Based on the rapid promotion of"Maritime Silk Road"and the proposal of the Belt and Road initiative by China,this empirical study established the initiative of"Maritime Silk Road"economic entity through the participation of small and medium enterprises in trades along the maritime silk road.The study filled the gap of research on maritime economy in China,put forward the"maritime economy"priority development initiative,and thus discovered whether it's feasible to promote regional integration-the inspiration of maritime economy;it is of great significance in the economic sense to find out that how to face the interference of trade related to leading powers,and the inspiration of the manufacturing industry for maritime economy along the Belt and Road.
基金the staged achievement of the special project of liberal arts of the social sciences fund of Xi’an(14wl08)
文摘For Xi'an,the central city of the Silk Road Economic Belt,it is both the theoretical and practical problem to develop into an international metropolis,so how to develop into an international metropolis becomes the research focus. This paper firstly introduces the necessity,the current situation and the evaluation index system of the construction of the city Xi'an in the context of the Silk Road Economic Belt; then it analyzes the econom ic developm ent of the three old districts,the three new districts,the three suburban districts and the four counties; and finally it puts forward the development path for Xi'an in the context of the Silk Road Economic Belt — the development pattern of "five districts,one international harbor area and two bases""nine-grid-pattern "economic development and the function-oriented coordinated development of the regional economy.
基金the staged achievement of 2014 social sciences funded program of Education Department of Shaanxi Provincial Government-"Establishment of Bilingual Corpus of World Heritage Sites in Shaanxi Province"(14JK1768)
文摘Translation,as a medium of globalization and the means of overcoming the Babylonian curse,is approached differently by varied translation theories,among which the Skopos approach seems to the one that guides the translation of the nomination dossier in an implicit manner.The dossier,distinct from other genres of texts,has a very clear function which explains why the Skopos theory plays an invisible hand throughout its translation.
