A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s...A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.展开更多
Behaviors of sika deer in spring were studied by scan sampling, ad libitum sampling, and all-occurrence recording methods during 1998. The results showed that behaviors of sika deer in spring can be classified by seve...Behaviors of sika deer in spring were studied by scan sampling, ad libitum sampling, and all-occurrence recording methods during 1998. The results showed that behaviors of sika deer in spring can be classified by seven categories: grazing, ruminating, bedding, moving, standing, drinking, alert, agonistic and other behaviors. Various behavioral models were more regular. Grazing behavior was a kind of mainly behavioral model.展开更多
[目的]探究梅花鹿富含半胱氨酸和甘氨酸蛋白3(cysteine and glycine-rich protein 3,CSRP3)基因的生物学作用及其在不同肌肉组织中的表达情况。[方法]以梅花鹿背最长肌为试验材料,克隆获得CSRP3基因,测序后进行相似性比对,构建系统进化...[目的]探究梅花鹿富含半胱氨酸和甘氨酸蛋白3(cysteine and glycine-rich protein 3,CSRP3)基因的生物学作用及其在不同肌肉组织中的表达情况。[方法]以梅花鹿背最长肌为试验材料,克隆获得CSRP3基因,测序后进行相似性比对,构建系统进化树,并对CSRP3蛋白进行生物信息学分析。运用实时荧光定量PCR技术来研究梅花鹿背最长肌、臀大肌、股四头肌、肋间肌中CSRP3基因的表达差异。[结果]成功克隆出梅花鹿CSRP3基因,其编码区片段大小为585 bp,共编码194个氨基酸。BLAST比对结果显示,梅花鹿CSRP3基因与马鹿的相似性最高,为99.83%,与白尾鹿、牛、羊的相似性均超过95%,说明梅花鹿CSRP3基因在进化中具有高度保守性;根据梅花鹿CSRP3基因构建系统进化树,结果表明,梅花鹿与马鹿的亲缘关系最近,与虎和虎鲸的亲缘关系最远。CSRP3基因编码蛋白分子式为C_(903)H_(1403)N_(263)O_(275)S_(19),相对分子质量为20 952.81,理论等电点(pI)为8.89,脂肪系数为43.81,其二级结构以不规则卷曲为主;亚细胞定位于细胞核内。实时荧光定量PCR结果表明,CSRP3基因在梅花鹿不同部位肌肉组织中均有表达,在梅花鹿肋间肌中的表达量最高,与背最长肌、臀大肌、股四头肌存在显著性差异(P<0.05),而背最长肌、臀大肌和股四头肌之间CSRP3基因表达量差异并不显著(P>0.05)。[结论]本研究成功克隆了梅花鹿CSRP3基因,其编码蛋白位于细胞核内;CSRP3基因在梅花鹿不同肌肉组织中存在差异表达。本研究结果为CSRP3基因在梅花鹿肉品质方面的调控机制研究提供了数据基础。展开更多
【目的】通过分析梅花鹿早期妊娠不同时期的血液转录组数据,筛选参与梅花鹿早期妊娠的关键基因。【方法】分别采集人工授精后0、7、15和20 d的雌性梅花鹿血液样品,利用Illumina HiSeq TM 3000C测序平台进行转录组分析。对不同时期的血...【目的】通过分析梅花鹿早期妊娠不同时期的血液转录组数据,筛选参与梅花鹿早期妊娠的关键基因。【方法】分别采集人工授精后0、7、15和20 d的雌性梅花鹿血液样品,利用Illumina HiSeq TM 3000C测序平台进行转录组分析。对不同时期的血液样本进行主成分分析和组间表达相关性分析,鉴定梅花鹿早期妊娠的关键阶段,利用DESeq筛选各时期的差异表达基因(DEGs),并进行GO和KEGG功能分析以及蛋白互作网络构建。【结果】转录组测序结果显示,妊娠第15天时梅花鹿血液出现了转录高峰,比较组D7 vs D0、D15 vs D7、D20 vs D15分别筛选到181、996、265个DEGs。其中比较组D7 vs D0共筛选到ERAS、MET、KDR等7个关键基因。综合GO和KEGG功能富集结果显示,基因主要富集在细胞对刺激的响应、信号转导等生物过程,以及PI3K-Akt信号通路、MAPK信号通路等通路中;比较组D15 vs D7共筛选到HRAS、HIF 1A、MAPK 1、JAK 1等10个关键基因,主要富集在免疫系统进程、免疫反应、趋化因子信号通路、C型凝集素受体信号通路等信号通路中;比较组D20 vs D15共筛选到MAPK 1、MMP 2等4个关键基因,主要富集在雌激素信号通路和松弛素信号通路中。【结论】本研究分析了梅花鹿早期妊娠关键时间点血液转录组的动态表达,筛选到KDR、HRAS、MAPK 1、JAK 1等关键基因,为梅花鹿早期妊娠诊断标志物的挖掘提供参考。展开更多
文摘A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.
文摘Behaviors of sika deer in spring were studied by scan sampling, ad libitum sampling, and all-occurrence recording methods during 1998. The results showed that behaviors of sika deer in spring can be classified by seven categories: grazing, ruminating, bedding, moving, standing, drinking, alert, agonistic and other behaviors. Various behavioral models were more regular. Grazing behavior was a kind of mainly behavioral model.
文摘[目的]探究梅花鹿富含半胱氨酸和甘氨酸蛋白3(cysteine and glycine-rich protein 3,CSRP3)基因的生物学作用及其在不同肌肉组织中的表达情况。[方法]以梅花鹿背最长肌为试验材料,克隆获得CSRP3基因,测序后进行相似性比对,构建系统进化树,并对CSRP3蛋白进行生物信息学分析。运用实时荧光定量PCR技术来研究梅花鹿背最长肌、臀大肌、股四头肌、肋间肌中CSRP3基因的表达差异。[结果]成功克隆出梅花鹿CSRP3基因,其编码区片段大小为585 bp,共编码194个氨基酸。BLAST比对结果显示,梅花鹿CSRP3基因与马鹿的相似性最高,为99.83%,与白尾鹿、牛、羊的相似性均超过95%,说明梅花鹿CSRP3基因在进化中具有高度保守性;根据梅花鹿CSRP3基因构建系统进化树,结果表明,梅花鹿与马鹿的亲缘关系最近,与虎和虎鲸的亲缘关系最远。CSRP3基因编码蛋白分子式为C_(903)H_(1403)N_(263)O_(275)S_(19),相对分子质量为20 952.81,理论等电点(pI)为8.89,脂肪系数为43.81,其二级结构以不规则卷曲为主;亚细胞定位于细胞核内。实时荧光定量PCR结果表明,CSRP3基因在梅花鹿不同部位肌肉组织中均有表达,在梅花鹿肋间肌中的表达量最高,与背最长肌、臀大肌、股四头肌存在显著性差异(P<0.05),而背最长肌、臀大肌和股四头肌之间CSRP3基因表达量差异并不显著(P>0.05)。[结论]本研究成功克隆了梅花鹿CSRP3基因,其编码蛋白位于细胞核内;CSRP3基因在梅花鹿不同肌肉组织中存在差异表达。本研究结果为CSRP3基因在梅花鹿肉品质方面的调控机制研究提供了数据基础。
文摘【目的】通过分析梅花鹿早期妊娠不同时期的血液转录组数据,筛选参与梅花鹿早期妊娠的关键基因。【方法】分别采集人工授精后0、7、15和20 d的雌性梅花鹿血液样品,利用Illumina HiSeq TM 3000C测序平台进行转录组分析。对不同时期的血液样本进行主成分分析和组间表达相关性分析,鉴定梅花鹿早期妊娠的关键阶段,利用DESeq筛选各时期的差异表达基因(DEGs),并进行GO和KEGG功能分析以及蛋白互作网络构建。【结果】转录组测序结果显示,妊娠第15天时梅花鹿血液出现了转录高峰,比较组D7 vs D0、D15 vs D7、D20 vs D15分别筛选到181、996、265个DEGs。其中比较组D7 vs D0共筛选到ERAS、MET、KDR等7个关键基因。综合GO和KEGG功能富集结果显示,基因主要富集在细胞对刺激的响应、信号转导等生物过程,以及PI3K-Akt信号通路、MAPK信号通路等通路中;比较组D15 vs D7共筛选到HRAS、HIF 1A、MAPK 1、JAK 1等10个关键基因,主要富集在免疫系统进程、免疫反应、趋化因子信号通路、C型凝集素受体信号通路等信号通路中;比较组D20 vs D15共筛选到MAPK 1、MMP 2等4个关键基因,主要富集在雌激素信号通路和松弛素信号通路中。【结论】本研究分析了梅花鹿早期妊娠关键时间点血液转录组的动态表达,筛选到KDR、HRAS、MAPK 1、JAK 1等关键基因,为梅花鹿早期妊娠诊断标志物的挖掘提供参考。
