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美国博士研究生拔尖创新人才培养的跨学科创新及其启示——基于斯坦福大学SHIPS项目的案例考察 被引量:2
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作者 田贤鹏 史洁 《研究生教育研究》 北大核心 2025年第4期110-117,共8页
在全球化的人才竞争中,具有跨学科协作能力的博士研究生拔尖创新人才备受青睐,如何推动拔尖创新人才培养的跨学科创新已成为世界各国高校面临的迫切议题。以斯坦福大学SHIPS博士项目为例,深入考察博士研究生拔尖创新人才培养的跨学科机... 在全球化的人才竞争中,具有跨学科协作能力的博士研究生拔尖创新人才备受青睐,如何推动拔尖创新人才培养的跨学科创新已成为世界各国高校面临的迫切议题。以斯坦福大学SHIPS博士项目为例,深入考察博士研究生拔尖创新人才培养的跨学科机制及其深层影响机制。结果发现:美国博士研究生拔尖创新人才培养的跨学科机制呈现四方面特征:一是“跨学科+问题”双向驱动的跨学科理念引领;二是“母学科+跨学科”矩阵组合的跨学科知识整合;三是“厚基础+强思维”互补嵌入的跨学科课程供给;四是“内协同+外联动”资源聚集的跨学科系统支持。研究认为,博士研究生拔尖创新人才培养的跨学科机制构建应当始终贯穿“学科为基、问题导向”的跨学科复合育人理念;强化“横向扩展、纵向深入”的跨学科课程群建设;着力突破“院系为界、学段壁垒”的跨学科组织限制;全面建设“政校企社、协同共育”的跨学科系统生态。 展开更多
关键词 跨学科 ships项目 拔尖创新人才 斯坦福大学 博士研究生
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Numerical Modeling of Ship-Ice-Water Interaction for Freerunning Ships in Pack Ice
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作者 ZOU Ming ZOU Zao−jian +1 位作者 ZOU Lu ZHU Sheng−tao 《船舶力学》 北大核心 2025年第6期878-887,共10页
Ice-going ships play a crucial role in polar transportation and resource extraction.Different from the existing modeling approach which assumes that ships remain stationary,dynamic overset grid technology and DFBI(Dyn... Ice-going ships play a crucial role in polar transportation and resource extraction.Different from the existing modeling approach which assumes that ships remain stationary,dynamic overset grid technology and DFBI(Dynamic Fluid-Body Interaction)method are employed in this paper to enable the free-running motion of the ship in modeling.A numerical model capable of simulating a ship navigating through pack ice area is proposed,which uses Computational Fluid Dynamics(CFD)method to solve the flow field and applies the Discrete Element Method(DEM)to simulate ship-ice and ice-ice interactions.Besides,the proposed high-precision method for generating pack ice area can be used in conjunction with the proposed numerical model.By comparing the numerical results with the available model test data and experimental observations,the effectiveness of the numerical model is validated,demonstrating its strong capability of predicting resistance and simulating ship navigation in pack ice,as well as its significant potential and applicability for further studies. 展开更多
关键词 pack ice ship-ice-water interaction CFD-DEM dynamic overset grid technology ship resistance
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Adaptive robust dissipative designs on straight path control for underactuated ships 被引量:3
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作者 Li Tieshan Yang Yansheng Hong Biguang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2006年第1期177-181,共5页
An adaptive robust control algorithm for ship straight path control system in the presence of both modeling uncertainties and the bounded disturbances is proposed. Motivated by the backstepping approach, the algorithm... An adaptive robust control algorithm for ship straight path control system in the presence of both modeling uncertainties and the bounded disturbances is proposed. Motivated by the backstepping approach, the algorithm is developed by using the dissipation theory, such that the resulting dosed-loop system is both strictly dissipative and asymptotically adaptively stable for all admissible uncertainties. Also, it is able to steer an underactuated ship along a prescribed straight path with ultimate bounds under external disturbances induced by wave, wind and ocean current. When there are no disturbances, the straight path control can be implemented in a locally asymptotically stable manner. Simulation results on an ocean-going training ship ‘YULONG' are presented to validate the effectiveness of the algorithm. 展开更多
关键词 underactuated ship straight path control nonlinear system adaptive backstepping design robust control.
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Multi-static InISAR imaging for ships under sparse aperture 被引量:3
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作者 JI Bingren WANG Yong +1 位作者 ZHAO Bin XU Rongqing 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2022年第3期575-584,共10页
This paper concentrates on super-resolution imaging of the ship target under the sparse aperture situation.Firstly,a multi-static configuration is utilized to solve the coherent processing interval(CPI)problem caused ... This paper concentrates on super-resolution imaging of the ship target under the sparse aperture situation.Firstly,a multi-static configuration is utilized to solve the coherent processing interval(CPI)problem caused by the slow-speed motion of ship targets.Then,we realize signal restoration and image reconstruction with the alternating direction method of multipliers(ADMM).Furthermore,we adopt the interferometric technique to produce the three-dimensional(3D)images of ship targets,namely interferometric inverse synthetic aperture radar(InISAR)imaging.Experiments based on the simulated data are utilized to verify the validity of the proposed method. 展开更多
关键词 multi-static sparse aperture signal recovery inter-ferometric inverse synthetic aperture radar(InISAR) ship target alternating direction method of multipliers(ADMM)
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Sliding-mode control of path following for underactuated ships based on high gain observer 被引量:1
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作者 秦梓荷 林壮 +1 位作者 孙寒冰 杨东梅 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第12期3356-3364,共9页
A nonlinear robust control strategy is proposed to force an underactuated surface ship to follow a predefined path with uncertain environmental disturbance and parameters.In the controller design,a high-gain observer ... A nonlinear robust control strategy is proposed to force an underactuated surface ship to follow a predefined path with uncertain environmental disturbance and parameters.In the controller design,a high-gain observer is used to estimate velocities,thus only position and yaw angle measurements are required.The control problem of underactuated system is transformed into a control of fully actuated system through adopting an improved line-of-sight(LOS) guidance law.A sliding-mode controller is designed to eliminate the yaw angle error,and provide the control system robustness.The control law is proved semi-globally exponentially stable(SGES) by applying Lyapunov stability theory,and numerical simulation using real data of a monohull ship illustrates the effectiveness and robustness of the proposed methodology. 展开更多
关键词 underactuated ship path following sliding-mode control line-of-sight guidance high gain observer
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Adaptive back-stepping control on container ships for path following 被引量:1
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作者 ZHAO Yang DONG Lili 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2020年第4期780-790,共11页
A feedback-dominance based adaptive back-stepping(FDBAB) controller is designed to drive a container ship to follow a predefined path. In reality, current, wave and wind act on the ship and produce unwanted disturbanc... A feedback-dominance based adaptive back-stepping(FDBAB) controller is designed to drive a container ship to follow a predefined path. In reality, current, wave and wind act on the ship and produce unwanted disturbances to the ship control system.The FDBAB controller has to compensate for such disturbances and steer the ship to track the predefined(or desired) path. The difference between the actual and the desired path along which the ship is to sail is defined as the tracking error. The FDBAB controller is built on the tracking error model which is developed based on Serret-Frenet frame transformation(SFFT). In additional to being affected by external disturbances, the ship has more outputs than inputs(under-actuated), and is inherently nonlinear.The back-stepping controller in FDBAB is used to compensate the nonlinearity. The adaptive algorithms in FDBAB is employed to approximate disturbances. Lyapunov's direct method is used to prove the stability of the control system. The FDBAB controlled system is implemented in Matlab/Simulink. The simulation results verify the effectiveness of the controller in terms of successful path tracking and disturbance rejection. 展开更多
关键词 under-actuated NONLINEAR environmental disturbance path following Serret-Frenet frame transformation(SFFT) ship steering
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Energy Efficiency Operating Indicator Forecasting and Speed Design Optimization for Polar Ice Class Merchant Vessels
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作者 LU Yu LI Chen−ran +3 位作者 ZHU Xiang−hang LI Shi−an GU Zhu−hao LIU She−wen 《船舶力学》 北大核心 2025年第6期901-911,共11页
In order to accurately forecast the main engine fuel consumption and reduce the Energy Efficiency Operational Indicator(EEOI)of merchant ships in polar ice areas,the energy transfer relationship between ship-machine-p... In order to accurately forecast the main engine fuel consumption and reduce the Energy Efficiency Operational Indicator(EEOI)of merchant ships in polar ice areas,the energy transfer relationship between ship-machine-propeller is studied by analyzing the complex force situation during ship navigation and building a MATLAB/Simulink simulation platform based on multi-environmental resistance,propeller efficiency,main engine power,fuel consumption,fuel consumption rate and EEOI calculation module.Considering the environmental factors of wind,wave and ice,the route is divided into sections,the calculation of main engine power,main engine fuel consumption and EEOI for each section is completed,and the speed design is optimized based on the simulation model for each section.Under the requirements of the voyage plan,the optimization results show that the energy efficiency operation index of the whole route is reduced by 3.114%and the fuel consumption is reduced by 9.17 t. 展开更多
关键词 Energy Efficiency Operational Indicator ice-class ships segment division design optimization
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食管鳞状细胞癌中的SHIP2表达及其与细胞增殖、迁移和侵袭的关系
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作者 陈双双 杨莹 +2 位作者 李萍 陈希贤 刘红春 《临床与实验病理学杂志》 北大核心 2025年第7期868-875,885,共9页
目的 探讨SHIP2在食管鳞状细胞癌(esophageal squamous cell carcinoma, ESCC)中的表达及其对ESCC细胞恶性生物学行为的影响。方法 利用UALCAN数据库分析SHIP2在食管癌中的表达,应用qRT-PCR和免疫组化SP法分析ESCC组织中SHIP2的表达,通... 目的 探讨SHIP2在食管鳞状细胞癌(esophageal squamous cell carcinoma, ESCC)中的表达及其对ESCC细胞恶性生物学行为的影响。方法 利用UALCAN数据库分析SHIP2在食管癌中的表达,应用qRT-PCR和免疫组化SP法分析ESCC组织中SHIP2的表达,通过qRT-PCR和Western blot检测SHIP2在人正常食管上皮细胞(human esophageal epithelial cells, HEEC)和ESCC细胞(KYSE150和EC109)中的表达情况。将KYSE150和EC109细胞分别分为两组:转染阴性对照siRNA组(NC组)和转染靶向SHIP2的siRNA组(si-SHIP2组)。采用CCK-8实验、EdU实验、划痕实验及Transwell实验,观察细胞的增殖、迁移和侵袭情况。通过Western blot检测上皮-间充质转化(epithelial-mesenchymal transition, EMT)相关蛋白(E-cadherin、N-cadherin和vimentin)的表达。结果 UALCAN数据库显示SHIP2在食管癌中的表达高于正常组织(P<0.05)。SHIP2在ESCC癌组织中的mRNA(2.19±3.20)表达和染色评分(5.33±3.83)均高于癌旁组织(1.00±0.80;0.87±1.07,P均<0.05)。SHIP2在ESCC细胞系中的mRNA(KYSE150、EC109:1.91±0.22、3.73±1.06)和蛋白(KYSE150、EC109:0.93±0.12、1.05±0.13)表达均高于HEEC(1.06±0.40;0.31±0.04,P均<0.05)。细胞功能实验显示,与NC组(CCK-8实验:KYSE150、EC109:2.44±0.12、3.56±0.07,EdU实验:KYSE150、EC109:44.46±4.74、38.82±3.79;划痕实验:KYSE150、EC109:0.85±0.07、0.70±0.06,Transwell迁移实验:KYSE150、EC109:130.30±9.53、39.25±3.30;Transwell侵袭实验:KYSE150、EC109:121.00±9.54、88.67±6.66)相比,si-SHIP2组细胞的增殖(CCK-8实验:KYSE150、EC109:1.56±0.03、2.85±0.02,EdU实验:KYSE150、EC109:19.34±6.24、17.39±1.14)、迁移(划痕实验:KYSE150、EC109:0.51±0.09、0.36±0.02,Transwell迁移实验:KYSE150、EC109:71.50±12.07、20.75±2.99)和侵袭(Transwell侵袭实验:KYSE150、EC109:73.33±4.04、12.67±2.31)能力均明显降低(P均<0.05)。Western blot结果显示,与NC组(0.48±0.21、0.42±0.24;1.00±0.04、1.17±0.18;1.34±0.10、1.00±0.13)相比,si-SHIP2组E-cadherin蛋白(1.10±0.22、1.02±0.20)表达增加(P<0.05),而N-cadherin蛋白(0.59±0.20、0.84±0.08)和vimentin蛋白(0.41±0.06、0.338±0.19)表达降低(P均<0.05)。结论 SHIP2在ESCC中高表达,下调SHIP2表达能够抑制ESCC细胞的增殖、迁移和侵袭行为。 展开更多
关键词 食管肿瘤 鳞状细胞癌 SHIP2 上皮-间充质转化 侵袭
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A New Approach for Melnikov Analysis of the Stability of a Ship with Water on Deck
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作者 WANG Ying−guang 《船舶力学》 北大核心 2025年第6期912-923,共12页
To study the rolling motion of a ship in the presence of water on its deck,a linear-plus-quadratic damping term was incorporated into its equation of motion.Ship model tests indicates that the key dynamics of the phys... To study the rolling motion of a ship in the presence of water on its deck,a linear-plus-quadratic damping term was incorporated into its equation of motion.Ship model tests indicates that the key dynamics of the physical system are preserved in the ship rolling equation with the linear-plus-quadratic type damping term.To take into account the presence of randomness in the excitation and the response,a new method was developed and a Melnikov criterion was obtained to provide an upper bound on the domain of the potential chaotic rolling motion(erratic rocking).Additionally,the Melnikov criterion proposed in this study was verified by the utilization of phase plane diagrams and Poincare maps.Furthermore,this research has made the initial endeavor to systematically modify the system parameters in the rolling equation of motion for ship stability analysis. 展开更多
关键词 ship stability Melnikov criterion erratic rocking Poincare section
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Surface Pressure Loading Technology of Ship Structures 被引量:2
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作者 DAI Ze-yu WEI Peng-yu +3 位作者 CHEN Xiao-ping JIANG Ze CHEN Zhe TANG Qin 《船舶力学》 EI CSCD 北大核心 2024年第12期1940-1952,共13页
A hull structure is prone to local deformation and damage due to the pressure load on the surface.How to simulate surface pressure is an important issue in ship structure test.The loading mode of hydraulic actuator co... A hull structure is prone to local deformation and damage due to the pressure load on the surface.How to simulate surface pressure is an important issue in ship structure test.The loading mode of hydraulic actuator combined with high-pressure flexible bladder was proposed,and the numerical model of the loading device based on flexible bladder was established.The design and analysis method of high-pressure flexible bladder based on aramid-fiber reinforced thermoplastic polyurethane was proposed to break through the surface pressure loading technology of ship structures.The surface pressure loading system based on flexible bladder was developed.The ultimate strength verification test of the box girder under the combined action of bending moment and pressure was carried out to systematically verify the feasibility and applicability of the loading system.The results show that the surface pressure loading technology can be used well for applying uniform pressure to ship structures.Compared with the traditional surface loading methods,the improved device can be applied with horizontal constant pressure load,with rapid response and safe process,and the pressure load is always stable with the increase of the bending moment load during the test.The requirement for uniform loading in the comprehensive strength test of large structural models is satisfied and the accuracy of the test results is improved by this system. 展开更多
关键词 surface pressure load loading system ship structure strength test flexible bladder
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A Framework of LSTM Neural Network Model in Multi-Time Scale Real-Time Prediction of Ship Motions in Head Waves 被引量:1
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作者 CHEN Zhan-yang ZHAN Zheng-yong +2 位作者 CHANG Shao-ping XU Shao-feng LIU Xing-yun 《船舶力学》 EI CSCD 北大核心 2024年第12期1803-1819,共17页
Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive act... Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive activities.However,the obvious memory effect of ship motion time series brings certain difficulty to rapid and accurate prediction.Therefore,a real-time framework based on the Long-Short Term Memory(LSTM)neural network model is proposed to predict ship motions in regular and irregular head waves.A 15000 TEU container ship model is employed to illustrate the proposed framework.The numerical implementation and the real-time ship motion prediction in irregular head waves corresponding to the different time scales are carried out based on the container ship model.The related experimental data were employed to verify the numerical simulation results.The results show that the proposed method is more robust than the classical extreme short-term prediction method based on potential flow theory in the prediction of nonlinear ship motions. 展开更多
关键词 deep learning LSTM ship motion real-time prediction irregular waves
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Novel method for extraction of ship target with overlaps in SAR image via EM algorithm 被引量:1
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作者 CAO Rui WANG Yong 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第4期874-887,共14页
The quality of synthetic aperture radar(SAR)image degrades in the case of multiple imaging projection planes(IPPs)and multiple overlapping ship targets,and then the performance of target classification and recognition... The quality of synthetic aperture radar(SAR)image degrades in the case of multiple imaging projection planes(IPPs)and multiple overlapping ship targets,and then the performance of target classification and recognition can be influenced.For addressing this issue,a method for extracting ship targets with overlaps via the expectation maximization(EM)algorithm is pro-posed.First,the scatterers of ship targets are obtained via the target detection technique.Then,the EM algorithm is applied to extract the scatterers of a single ship target with a single IPP.Afterwards,a novel image amplitude estimation approach is pro-posed,with which the radar image of a single target with a sin-gle IPP can be generated.The proposed method can accom-plish IPP selection and targets separation in the image domain,which can improve the image quality and reserve the target information most possibly.Results of simulated and real mea-sured data demonstrate the effectiveness of the proposed method. 展开更多
关键词 expectation maximization(EM)algorithm image processing imaging projection plane(IPP) overlapping ship tar-get synthetic aperture radar(SAR)
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Numerical Simulation of Multiple Collisions Between Ice Ridge and Polar Ship
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作者 JIA Bin JU Lei +2 位作者 SHI Li WANG Qing PANG Fu-zhen 《船舶力学》 EI CSCD 北大核心 2024年第12期1908-1924,共17页
Ships navigating in ice-covered regions will inevitably collide with ice ridges.Compared to other ice bodies,ice ridges exhibit more complicated mechanical behaviors due to the scale and structure characteristics.In t... Ships navigating in ice-covered regions will inevitably collide with ice ridges.Compared to other ice bodies,ice ridges exhibit more complicated mechanical behaviors due to the scale and structure characteristics.In this paper,nonlinear finite element method is used to investigate the interaction between a polar ship and an ice ridge.The ice ridge is modelled as elastic-plastic material based on Drucker-Prager yield function,with the consideration of the influence of cohesion,friction angle and material hardening.The material model is developed in LS-DYNA and solved using semi-implicit mapping algorithm.The stress distribution of ice ridge and ship,and the ice load history are evaluated through the simulation of multiple collisions.In addition,parametric analysis is performed to investigate the influence of ridge thickness and impact velocity on the ice load and energy absorption. 展开更多
关键词 ice ridge SHIP nonlinear FEM ice load interaction STRENGTH
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Theoretical and Experimental Research of High-Static-Low Dynamic Torsional Vibration Isolator for Ship Shafting
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作者 LI Lin-tao LU Jia-zhong +2 位作者 YANG Zhi-rong XIAO Wang-qiang RAO Zhu-shi 《船舶力学》 EI CSCD 北大核心 2024年第12期1970-1982,共13页
High-static-low-dynamic stiffness (HSLDS) vibration isolators have been demonstrated to be an effective means of attenuating low-frequency vibrations, and may be utilized for ship shafting applications to mitigate tor... High-static-low-dynamic stiffness (HSLDS) vibration isolators have been demonstrated to be an effective means of attenuating low-frequency vibrations, and may be utilized for ship shafting applications to mitigate torsional vibration. This paper presents the construction of a highly compact HSLDS torsional vibration isolator by connecting positive and negative stiffness components in paral lel. Based on mechanical model analysis, the restoring torque of negative stiffness components is de rived from their springs and connecting rods, while that of positive stiffness components is obtained through their circular section flexible rods. The quasizero stiffness characteristics of the HSLDS iso lator are achieved through a combination of static structural simulation and experimental test. The tor sional vibration isolation performance is assessed by means of numerical simulation and theory analy sis. Finally, the frequency-sweep vibration test is conducted. The test results indicate that the HSLDS torsional vibration isolator exhibits superior low-frequency isolation performance compared to its linear counterpart, rendering it a promising solution for mitigating low-frequency torsional vi bration in ship shafting. 展开更多
关键词 ship shafting high-static-low-dynamic stiffness torsional vibration isolator
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Ship recognition based on HRRP via multi-scale sparse preserving method
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作者 YANG Xueling ZHANG Gong SONG Hu 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期599-608,共10页
In order to extract the richer feature information of ship targets from sea clutter, and address the high dimensional data problem, a method termed as multi-scale fusion kernel sparse preserving projection(MSFKSPP) ba... In order to extract the richer feature information of ship targets from sea clutter, and address the high dimensional data problem, a method termed as multi-scale fusion kernel sparse preserving projection(MSFKSPP) based on the maximum margin criterion(MMC) is proposed for recognizing the class of ship targets utilizing the high-resolution range profile(HRRP). Multi-scale fusion is introduced to capture the local and detailed information in small-scale features, and the global and contour information in large-scale features, offering help to extract the edge information from sea clutter and further improving the target recognition accuracy. The proposed method can maximally preserve the multi-scale fusion sparse of data and maximize the class separability in the reduced dimensionality by reproducing kernel Hilbert space. Experimental results on the measured radar data show that the proposed method can effectively extract the features of ship target from sea clutter, further reduce the feature dimensionality, and improve target recognition performance. 展开更多
关键词 ship target recognition high-resolution range profile(HRRP) multi-scale fusion kernel sparse preserving projection(MSFKSPP) feature extraction dimensionality reduction
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舰艇用LZN-2型高性能阻尼材料释放物定性定量分析与评价 被引量:1
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作者 许林军 陈茜 +3 位作者 汪南平 徐新宏 燕锐 方晶晶 《分析测试学报》 CAS CSCD 北大核心 2004年第z1期181-182,186,共3页
  舰艇舱室多为密闭环境,舱室内使用的非金属材料释放物是舱室污染的主要来源,因此对所选用的非金属材料必须进行常温释放物的定性定量分析.由于舰艇的特殊性,不仅要求非金属材料在常温条件下释放物的浓度符合舰艇舱室容许浓度,还要...   舰艇舱室多为密闭环境,舱室内使用的非金属材料释放物是舱室污染的主要来源,因此对所选用的非金属材料必须进行常温释放物的定性定量分析.由于舰艇的特殊性,不仅要求非金属材料在常温条件下释放物的浓度符合舰艇舱室容许浓度,还要求材料在高温条件(火灾或爆炸)下不产生高毒性的物质.…… 展开更多
关键词 LZN-2 High performance damping material Air composition ships
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慢性粒细胞性白血病中SHIP1基因的表达变化及机制探讨 被引量:10
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作者 刘小军 罗建民 +5 位作者 杨琳 温树鹏 姚丽 杜行严 杨敬慈 董作仁 《解放军医学杂志》 CAS CSCD 北大核心 2008年第6期701-703,共3页
目的观察SHIP1基因在慢性粒细胞性白血病(CML)患者中的表达变化,并通过特异性小干扰RNA(siRNA)封闭K562细胞株BCR-ABL基因的表达,探讨BCR-ABL基因表达对SHIP1基因的影响。方法应用RQ-PCR方法检测CML患者骨髓、正常人外周血白细胞及白血... 目的观察SHIP1基因在慢性粒细胞性白血病(CML)患者中的表达变化,并通过特异性小干扰RNA(siRNA)封闭K562细胞株BCR-ABL基因的表达,探讨BCR-ABL基因表达对SHIP1基因的影响。方法应用RQ-PCR方法检测CML患者骨髓、正常人外周血白细胞及白血病细胞株K562中SHIP1基因表达的差异。另取K562细胞分为3组:空白对照组(不加任何干扰成分);非特异性siRNA处理组(加非特异性siRNA);特异性siRNA处理组(加入特异性siRNA封闭BCR/ABL融合基因),应用RQ-PCR及Western blot方法分别检测3组中BCR/ABL、SHIP1基因的mRNA及其相应蛋白P210(BCR/ABL编码)、P145(SHIP1基因编码)的表达,并比较各组中表达水平的变化。结果CML患者骨髓白细胞和K562细胞中SHIP1基因的表达明显低于正常人的外周血白细胞。特异性siRNA处理组中BCR-ABL基因mRNA和P210蛋白的表达水平明显下降,SHIP1基因mRNA和P145蛋白表达水平随BCR-ABL表达下降而增加;非特异性siRNA处理组与空白组相比无明显差异。结论CML患者SHIP1基因的表达低于正常人;特异性siRNA能够抑制BCR-ABL基因的表达;BCR-ABL基因能抑制SHIP1基因及其蛋白表达。 展开更多
关键词 白血病 髓样 慢性 BCR/ABL基因 SHIP1基因 RNA 小分子干扰
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乳康饮对裸鼠乳腺癌组织PI3K/Akt信号通路相关因子表达的影响 被引量:11
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作者 李湘奇 王莹 +2 位作者 魏昌然 刘晓菲 李静蔚 《中华中医药学刊》 CAS 北大核心 2019年第7期1543-1547,共5页
目的:观察中药乳康饮对移植瘤小鼠乳腺癌PI3K/AKT信号通路相关因子表达的影响,探讨其抑制乳腺癌的作用机制。方法:60只BALB/c裸鼠乳腺原位种植人乳腺癌细胞株MDA-MB-231,建立乳腺癌移植瘤自发性转移模型,随机分成6组,分别为模型对照组,5... 目的:观察中药乳康饮对移植瘤小鼠乳腺癌PI3K/AKT信号通路相关因子表达的影响,探讨其抑制乳腺癌的作用机制。方法:60只BALB/c裸鼠乳腺原位种植人乳腺癌细胞株MDA-MB-231,建立乳腺癌移植瘤自发性转移模型,随机分成6组,分别为模型对照组,5-FU对照组、疏肝理气组、化痰散瘀组、益气健脾组、乳康饮组,每组10只。分别给予生理盐水、5-FU、乳康饮及各拆方(疏肝理气、化痰散瘀、益气健脾)药物灌胃,灌胃液体量均为0.4 mL/(d·只),均1次/d,连续6周后将裸鼠处死,检测裸鼠第2、3对乳腺原位肿瘤PI3K、Akt、PTEN、SHIP蛋白水平及mRNA的表达。结果:乳康饮及各拆方组药物对PI3K、Akt mRNA的表达均起到抑制作用,对PTEN、SHIP mRNA表达有促进作用,与模型对照组比较差异有统计学意义(均P<0.05或P<0.01),而益气健脾组和乳康饮全方效果更具优势;裸鼠乳腺癌组织PI3K、Akt、PTEN、SHIP蛋白水平的表达的检测结果,乳康饮组及各拆方组对PI3K、Akt蛋白的表达均有不同程度的抑制作用,对抑癌因子PTEN、SHIP蛋白的表达有促进作用,各拆方组之间也存在统计学差异,乳康饮的作用明显优于各拆方组(P<0.05或0.01)。结论:乳康饮可能通过抑制PI3K、Akt的表达,促进PTEN、SHIP的表达,调控PI3K/AKT信号通路,对乳腺癌的发生发展起到抑制作用。 展开更多
关键词 乳腺癌 乳康饮 磷脂酰肌醇3-激酶/丝苏氨酸蛋白激酶B信号通路 PTEN SHIP
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急性白血病细胞SHIP基因的突变分析(英文) 被引量:2
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作者 罗建民 刘泽林 +3 位作者 郝洪岭 王福旭 董作仁 大野竜三 《中国实验血液学杂志》 CAS CSCD 2004年第4期420-426,共7页
SHIP (SH2domaincontaininginositol5′ phosphatase)基因主要在造血细胞表达 ,并在造血细胞的发生发育中发挥关键的负调节作用。本研究旨在评价SHIP基因突变在白血病发病中的作用。利用RT PCR、SSCP及DNA序列分析技术检测了 32例急性... SHIP (SH2domaincontaininginositol5′ phosphatase)基因主要在造血细胞表达 ,并在造血细胞的发生发育中发挥关键的负调节作用。本研究旨在评价SHIP基因突变在白血病发病中的作用。利用RT PCR、SSCP及DNA序列分析技术检测了 32例急性髓细胞白血病、9例急性淋巴细胞白血病及正常对照骨髓或外周血标本中SHIP基因表达及突变情况。RT PCR显示所有标本中都有SHIP基因表达 ,2 2 % (7/32 )AML和 12 % (1/9)ALL标本中存在SHIP基因的突变 ,其中 1例AML患者发病时标本同时存在 2个错义突变 ,而完全缓解 (CR)后消失 ,而且其发病时的白血病细胞在体外随着IL 3的刺激其Akt的磷酸化明显增加。结论 :本研究首次发现急性白血病细胞中SHIP基因突变 ,提示SHIP基因的突变可能与白血病发病有关 ;在造血细胞中 ,它很可能做为一个抑癌基因通过负性调节PI3K/Akt信号通路发挥作用。 展开更多
关键词 SHIP基因 基因突变 急性白血病
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硼替佐米对K562细胞增殖、凋亡及SHIP基因表达的影响 被引量:2
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作者 贾志强 魏玉涛 +1 位作者 李爱明 成志勇 《中国实验血液学杂志》 CAS CSCD 北大核心 2013年第4期916-919,共4页
本研究旨在探讨蛋白酶体抑制剂硼替佐米(bortezomib)对K562细胞的增殖、凋亡及SHIP基因表达的影响。将不同浓度的硼替佐米作用于K562细胞,采用MTT法测定细胞增殖活性,用流式细胞术检测细胞凋亡,RT-PCR方法检测SHIP mRNA表达。结果表明,... 本研究旨在探讨蛋白酶体抑制剂硼替佐米(bortezomib)对K562细胞的增殖、凋亡及SHIP基因表达的影响。将不同浓度的硼替佐米作用于K562细胞,采用MTT法测定细胞增殖活性,用流式细胞术检测细胞凋亡,RT-PCR方法检测SHIP mRNA表达。结果表明,硼替佐米10、20、50和100 nmol/L作用于K562细胞24 h,细胞增殖抑制率分别为(5.76±1.47)%、(10.55±1.59)%、(17.14±2.05)%和(27.69±3.57)%,空白对照组为(1.30±0.10)%;20nmol/L硼替佐米作用于K562细胞24、48、72 h,细胞增殖抑制率分别为(10.55±1.59)%、(16.33±2.53)%、(19.78±1.56)%,24 h组与48 h组比较,差异具有统计学意义(P<0.05),10、20、50、100 nmol/L硼替佐米作用于K562细胞24h,Annexin V-FITC/PI双标法显示其凋亡率分别为(12.7±0.6)%、(26.9±0.9)%、(32.6±1.2)%、(72.5±1.5)%,均高于对照组(1.0±0.5)%(P<0.05)。RT-PCR法检测结果显示应用硼替佐米作用后SHIP mRNA表达上调明显,与空白对照组比较差异有统计学意义。结论:硼替佐米呈时间、浓度依赖性抑制K562细胞增殖,并能通过上调SHIP基因的表达诱导细胞凋亡。 展开更多
关键词 硼替佐米 K562细胞 SHIP基因 白血病
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