There is increasing evidence that stress can activate the hypothalamic-pituitary-adrenal-axis and hypothalamic-pituitary- thyroid-axis, and further affect the synthesis and secretion of corticotrophin-releasing hormo...There is increasing evidence that stress can activate the hypothalamic-pituitary-adrenal-axis and hypothalamic-pituitary- thyroid-axis, and further affect the synthesis and secretion of corticotrophin-releasing hormone （CRH） and thyrotropin-releasing hormone （TRH）. To evaluate the effect of cold stress on the hypothalamic CRH and TRH messenger RNA （mRNA） levels in Yisha chickens, male Yisha chickens were subjected to acute （1, 6, 12 h） and chronic （5, 10, 20 d） cold stress （12±1）℃. Hypothalami were collected for assessment of mRNA levels by semi-quantitative RT-PCR. Acute stress resulted in a significant decrease of CRH mRNA levels at 6 and 12 h, and a significant increase of TRH mRNA levels at every stress time point. Chronic cold stress resulted in a significant increase of CRH mRNA levels and a significant decrease of TRH mRNA levels compared with the control group at every stress time point. The results suggest that the two genes differently respond to cold stress at the mRNA levels. And the different degrees of cold stress will produce different effects on the identical gene.展开更多
In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quan...In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quantitative RT-PCR using the housekeeping gene recF as an internal standard.The expression of ArcA was undetectable until about 3 hours under standard culture conditions,but it was promptly and highly expressed in anaerobic culture.The results are consistent with ArcA being a potential virulence gene of Actinobacillus pleuropneumoniae,and likely playing an important role in pathogenesis caused by this organism.展开更多
文摘There is increasing evidence that stress can activate the hypothalamic-pituitary-adrenal-axis and hypothalamic-pituitary- thyroid-axis, and further affect the synthesis and secretion of corticotrophin-releasing hormone （CRH） and thyrotropin-releasing hormone （TRH）. To evaluate the effect of cold stress on the hypothalamic CRH and TRH messenger RNA （mRNA） levels in Yisha chickens, male Yisha chickens were subjected to acute （1, 6, 12 h） and chronic （5, 10, 20 d） cold stress （12±1）℃. Hypothalami were collected for assessment of mRNA levels by semi-quantitative RT-PCR. Acute stress resulted in a significant decrease of CRH mRNA levels at 6 and 12 h, and a significant increase of TRH mRNA levels at every stress time point. Chronic cold stress resulted in a significant increase of CRH mRNA levels and a significant decrease of TRH mRNA levels compared with the control group at every stress time point. The results suggest that the two genes differently respond to cold stress at the mRNA levels. And the different degrees of cold stress will produce different effects on the identical gene.
基金supported by the Biotechnological Key Project of Sichuan Province,China(05NG020-016)The Yangtze River Scholar and Innovation Team develop Project of Ministry of Education,China(IRT0555-9)
文摘In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quantitative RT-PCR using the housekeeping gene recF as an internal standard.The expression of ArcA was undetectable until about 3 hours under standard culture conditions,but it was promptly and highly expressed in anaerobic culture.The results are consistent with ArcA being a potential virulence gene of Actinobacillus pleuropneumoniae,and likely playing an important role in pathogenesis caused by this organism.
