To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents （Dilu...To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents （Diluent I, Diluent II and Diluent III） and three boar breed semens （Yorkshire, Landrace and Duroc） were utilized. The sperm motility, effective survival time, survival index, catalase （CAT）, the total anti-oxidative capacity （T-AOC） and malondialdehyde （MDA） levels were evaluated. The results showed that there were significant interaction effects between diluents and breeds on the boar sperm motility （P〈0.001）, survival time （P〈0.001）, CAT levels （P〈0.001） and T-AOC levels （P〈0.001）, but neither effects nor interaction effects between diluents and breeds on survival index （P〉0.05）. All of the parameters varied significantly with the increase of the storage time （P〈0.001）. The survival time increased 12.9% in Yorkshire boar semen diluted with Diluent III than with Diluent II, while the survival time increased 6.6% in Landrace boar semen diluted with Diluent II than with Diluent III. Both CAT and T-AOC levels were significantly positive correlated with sperm motility in all the three boar breeds （P〈0.001）, while MDA levels were significantly negative correlated with sperm motility （P〈0.001）. These results indicated that Diluent III and Diluent II were the optimal commercial diluents for Yorkshire and Landrace boar semen stored at 17 ℃, respectively.展开更多
In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen i...In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI.展开更多
immature male mice were selected in the experiment to assess the effects of Deer semen on the development of testes. 20 mice were fed with 0.05 mL semen·d -1 for 15 days and the rest fed with the same volume...immature male mice were selected in the experiment to assess the effects of Deer semen on the development of testes. 20 mice were fed with 0.05 mL semen·d -1 for 15 days and the rest fed with the same volume of albumeh for the same period as controls. The relative weight of testis to body weight was higher in treated group than that in the control (P<0.05), so was the volume of testis (P<0.05). The density of testis was lower in treated group than in the control (P<0.05). Eighty percent of mice in the treated group showed the appearance of tubule cavity and spermatozoa in the testis, but only about 20% in the control. It was concluded that the Deer semen had improvement effects on the development of testes and sexual maturity of mice.展开更多
基金Supported by the National Swine Industry Technology System(CARS-36)the Scientifc and Technological Project of Yangling Demonstration Zone(2014NY-22)
文摘To investigate the effects of different diluents on the quality of the boar semen stored at 17℃, and assess the relationship between sperm motility and the relative levels of enzymes, three commercial diluents （Diluent I, Diluent II and Diluent III） and three boar breed semens （Yorkshire, Landrace and Duroc） were utilized. The sperm motility, effective survival time, survival index, catalase （CAT）, the total anti-oxidative capacity （T-AOC） and malondialdehyde （MDA） levels were evaluated. The results showed that there were significant interaction effects between diluents and breeds on the boar sperm motility （P〈0.001）, survival time （P〈0.001）, CAT levels （P〈0.001） and T-AOC levels （P〈0.001）, but neither effects nor interaction effects between diluents and breeds on survival index （P〉0.05）. All of the parameters varied significantly with the increase of the storage time （P〈0.001）. The survival time increased 12.9% in Yorkshire boar semen diluted with Diluent III than with Diluent II, while the survival time increased 6.6% in Landrace boar semen diluted with Diluent II than with Diluent III. Both CAT and T-AOC levels were significantly positive correlated with sperm motility in all the three boar breeds （P〈0.001）, while MDA levels were significantly negative correlated with sperm motility （P〈0.001）. These results indicated that Diluent III and Diluent II were the optimal commercial diluents for Yorkshire and Landrace boar semen stored at 17 ℃, respectively.
文摘In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI.
文摘immature male mice were selected in the experiment to assess the effects of Deer semen on the development of testes. 20 mice were fed with 0.05 mL semen·d -1 for 15 days and the rest fed with the same volume of albumeh for the same period as controls. The relative weight of testis to body weight was higher in treated group than that in the control (P<0.05), so was the volume of testis (P<0.05). The density of testis was lower in treated group than in the control (P<0.05). Eighty percent of mice in the treated group showed the appearance of tubule cavity and spermatozoa in the testis, but only about 20% in the control. It was concluded that the Deer semen had improvement effects on the development of testes and sexual maturity of mice.
