A new nanocomposite material for construction of glucose biosensor was prepared. The biosensor was formed by entrapping glucose oxidase(Gox) into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite fil...A new nanocomposite material for construction of glucose biosensor was prepared. The biosensor was formed by entrapping glucose oxidase(Gox) into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film. In this biosensing thin film, the multiwalled carbon nanotubes can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of glucose oxidase and exhibits a wide linear response range from 8 μmol/L to 3 mmol/L with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit of the biosensor are determined to be 6 s and 3.5 μmol/L, respectively. Another attractive characteristic is that the biosensor is inexpensive, stable and reliable.展开更多
A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and ...A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition ofpH 5.5, 0.8 V and 65℃.展开更多
A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to ...A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10 -6 to 2.4×10 -3 mol/L with detection limit of 1.0×10 -6 mol/L. The apparent Michaelis-Menten constant K m is found to be 3.2×10 -3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol -1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.展开更多
基金Project (20060532006) supported by Specialized Research Fund for the Doctoral Program of Higher Education
文摘A new nanocomposite material for construction of glucose biosensor was prepared. The biosensor was formed by entrapping glucose oxidase(Gox) into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film. In this biosensing thin film, the multiwalled carbon nanotubes can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of glucose oxidase and exhibits a wide linear response range from 8 μmol/L to 3 mmol/L with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit of the biosensor are determined to be 6 s and 3.5 μmol/L, respectively. Another attractive characteristic is that the biosensor is inexpensive, stable and reliable.
基金Projects(50473022, 20673036) supported by the National Natural Science Foundation of China project(2005) supported by the State Key Laboratory of Chemo/Biosensing and Chemometrics of China+1 种基金 project(2006FJ4100) supported by the Science Technology Project of Hunan Province project(2006) supported by the Postdoctor Foundation of Hunan University
文摘A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition ofpH 5.5, 0.8 V and 65℃.
文摘A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10 -6 to 2.4×10 -3 mol/L with detection limit of 1.0×10 -6 mol/L. The apparent Michaelis-Menten constant K m is found to be 3.2×10 -3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol -1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.
