【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference fo...【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.展开更多
The present study was conducted to see the short term impact of organic and inorganic fertilizers on soil microbial biomass both in spring and summer. Also aimed to observe the correlation between soil microbial bioma...The present study was conducted to see the short term impact of organic and inorganic fertilizers on soil microbial biomass both in spring and summer. Also aimed to observe the correlation between soil microbial biomass and soil DNA. The study concluded that type of fertilizer might alter the soil microbial biomass and DNA contents. In soil treated with organic fertilizers resulted in higher concentrations of microbial biomass and DNA contents in summer as compared to spring dute to increase in temperature. Correspondingly, in case of inorganic fertilizer, concentrations of soil microbial biomass and DNA detected higher in summer instead of spring. The statistical correlation between soil microbial biomass, DNA and ODR in spring and summer along with organic and inorganic fertilizers were calculated highly significant （p〉0.01）. This study demonstrated the impact of fertilizers and seasonal variations on soil microbial biomass and also revealed significant correlation between soil microbial biomass and soil DNA.展开更多
The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in He...The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in Heilongjiang Province. The application of soil phosphorus activator was able to increase the quantity of bacteria and fungi in soil, but its effect on actinomycetes in soil was not significant. The application of microbial inoculants increased the urease and sucrase activities in soil over the growing season, but only at the maturing stage soil acid phosphatase activity was enhanced with the applying soil phosphorus activator. The application of soil phosphorus activator increased alkali-hydrolyzable nitrogen and available phosphorus contents in soil, but did not increase available potassium content in soil. The optimal microbial inoculant application rate as applied as soil phosphorus activator was 7.5 kg hm-2.展开更多
[目的]为明确水肥管理和设施间作等措施对蔬菜土壤微生态及黄瓜、芹菜品质的影响。[方法]通过连续3 a的田间试验,采用连续水肥(CF,continuous fertilization in irrigation)和隔水一肥(AF,alternating fertilization in irrigation)2种...[目的]为明确水肥管理和设施间作等措施对蔬菜土壤微生态及黄瓜、芹菜品质的影响。[方法]通过连续3 a的田间试验,采用连续水肥(CF,continuous fertilization in irrigation)和隔水一肥(AF,alternating fertilization in irrigation)2种水肥模式,结合黄瓜单作(CU,cucumber monoculture)、芹菜单作(CE,celery monoculture)、黄瓜与芹菜间作(CC,cucumber and celery intercropping)3种种植模式,系统评估不同处理对作物品质、土壤理化性质和微生物群落的影响。[结果]CCCF处理显著提升黄瓜和芹菜的维生素C含量(提升16.6%~200%),而CUCF处理则在可溶性糖积累方面表现出优势。XGB模型分析揭示,速效钾和pH分别是调控维生素C和可溶性糖含量的关键因子。微生物组分析显示,间作处理富集特异性ASVs(7371~7526个),显著提高Steroidobacter、Flavobacterium等促生菌的丰度,同时抑制病原真菌的生长。功能预测表明,水肥管理主要影响氮循环功能,间作则增强甲烷营养功能。微生物网络分析进一步揭示,CF形成紧密的细菌互作网络,AF提高网络模块性,而CC则在保持高连接性的同时维持适度模块性。[结论]不同水肥和种植管理措施通过调控土壤理化性质、微生物群落结构和生态网络,对作物品质产生差异化影响。其中,CCCF和CUCF分别适合提升维生素C和可溶性糖含量,为设施农业精准调控作物品质提供新思路。展开更多
[目的]解决烤烟生长连作障碍。[方法]通过2021—2023年生物有机肥定位试验,研究连续3年生物有机肥施用对连作植烟土壤养分及微生多样性的影响。[结果]连作种植下连续3年施用生物有机肥的植烟土壤pH及有机质呈上升趋势,碱解氮含量降低,...[目的]解决烤烟生长连作障碍。[方法]通过2021—2023年生物有机肥定位试验,研究连续3年生物有机肥施用对连作植烟土壤养分及微生多样性的影响。[结果]连作种植下连续3年施用生物有机肥的植烟土壤pH及有机质呈上升趋势,碱解氮含量降低,有效磷及速效钾含量保持稳定。MiSeq高通量测序技术对植烟土壤细菌16S DNA V3~V4区域及真菌ITS ITS1的检测结果显示,连作种植下随着生物有机肥施用年限的增加,植烟土壤中的细菌及真菌OTUs数目、Chao1指数、香浓指数及物种数目呈增加趋势。在门水平上,连作植烟土壤酸杆菌门(Acidobacteria)丰富度随着生物有机肥施用年限的增加而增加,而拟杆菌门(Bacteroidetes)相对丰度减少;植烟土壤生物有机肥施用1年,子囊菌门(Ascomycota)丰富度最高,连续施用2年担子菌门(Basidiomycota)丰富度最高。[结论]生物有机肥能够降低连作植烟土壤酸化状况,提高土壤有机质含量,增加土壤微生物多样性,对稳定土壤速效养分具有一定的作用,为解决烟区连作障碍提供一定的理论依据。展开更多
基金Key Research project of Gansu Province of China(22YF7NA108)National Natural Science Foundation of China(31860549)+1 种基金Industry Supporting Project from Education Department of Gansu Province(2023CYZC-49)Major Science and Technology project of Gansu province(24ZDNA006)。
文摘【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.
基金Supported by the University Science and Technology Innovation Team Construction Projects of Heilongjiang Province(2013 TD003)
文摘The present study was conducted to see the short term impact of organic and inorganic fertilizers on soil microbial biomass both in spring and summer. Also aimed to observe the correlation between soil microbial biomass and soil DNA. The study concluded that type of fertilizer might alter the soil microbial biomass and DNA contents. In soil treated with organic fertilizers resulted in higher concentrations of microbial biomass and DNA contents in summer as compared to spring dute to increase in temperature. Correspondingly, in case of inorganic fertilizer, concentrations of soil microbial biomass and DNA detected higher in summer instead of spring. The statistical correlation between soil microbial biomass, DNA and ODR in spring and summer along with organic and inorganic fertilizers were calculated highly significant （p〉0.01）. This study demonstrated the impact of fertilizers and seasonal variations on soil microbial biomass and also revealed significant correlation between soil microbial biomass and soil DNA.
文摘The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in Heilongjiang Province. The application of soil phosphorus activator was able to increase the quantity of bacteria and fungi in soil, but its effect on actinomycetes in soil was not significant. The application of microbial inoculants increased the urease and sucrase activities in soil over the growing season, but only at the maturing stage soil acid phosphatase activity was enhanced with the applying soil phosphorus activator. The application of soil phosphorus activator increased alkali-hydrolyzable nitrogen and available phosphorus contents in soil, but did not increase available potassium content in soil. The optimal microbial inoculant application rate as applied as soil phosphorus activator was 7.5 kg hm-2.
文摘[目的]为明确水肥管理和设施间作等措施对蔬菜土壤微生态及黄瓜、芹菜品质的影响。[方法]通过连续3 a的田间试验,采用连续水肥(CF,continuous fertilization in irrigation)和隔水一肥(AF,alternating fertilization in irrigation)2种水肥模式,结合黄瓜单作(CU,cucumber monoculture)、芹菜单作(CE,celery monoculture)、黄瓜与芹菜间作(CC,cucumber and celery intercropping)3种种植模式,系统评估不同处理对作物品质、土壤理化性质和微生物群落的影响。[结果]CCCF处理显著提升黄瓜和芹菜的维生素C含量(提升16.6%~200%),而CUCF处理则在可溶性糖积累方面表现出优势。XGB模型分析揭示,速效钾和pH分别是调控维生素C和可溶性糖含量的关键因子。微生物组分析显示,间作处理富集特异性ASVs(7371~7526个),显著提高Steroidobacter、Flavobacterium等促生菌的丰度,同时抑制病原真菌的生长。功能预测表明,水肥管理主要影响氮循环功能,间作则增强甲烷营养功能。微生物网络分析进一步揭示,CF形成紧密的细菌互作网络,AF提高网络模块性,而CC则在保持高连接性的同时维持适度模块性。[结论]不同水肥和种植管理措施通过调控土壤理化性质、微生物群落结构和生态网络,对作物品质产生差异化影响。其中,CCCF和CUCF分别适合提升维生素C和可溶性糖含量,为设施农业精准调控作物品质提供新思路。
文摘[目的]解决烤烟生长连作障碍。[方法]通过2021—2023年生物有机肥定位试验,研究连续3年生物有机肥施用对连作植烟土壤养分及微生多样性的影响。[结果]连作种植下连续3年施用生物有机肥的植烟土壤pH及有机质呈上升趋势,碱解氮含量降低,有效磷及速效钾含量保持稳定。MiSeq高通量测序技术对植烟土壤细菌16S DNA V3~V4区域及真菌ITS ITS1的检测结果显示,连作种植下随着生物有机肥施用年限的增加,植烟土壤中的细菌及真菌OTUs数目、Chao1指数、香浓指数及物种数目呈增加趋势。在门水平上,连作植烟土壤酸杆菌门(Acidobacteria)丰富度随着生物有机肥施用年限的增加而增加,而拟杆菌门(Bacteroidetes)相对丰度减少;植烟土壤生物有机肥施用1年,子囊菌门(Ascomycota)丰富度最高,连续施用2年担子菌门(Basidiomycota)丰富度最高。[结论]生物有机肥能够降低连作植烟土壤酸化状况,提高土壤有机质含量,增加土壤微生物多样性,对稳定土壤速效养分具有一定的作用,为解决烟区连作障碍提供一定的理论依据。
