为探究胃肠消化对玉米活性肽α-葡萄糖苷酶抑制活性及抗氧化活性的影响,分别对消化前后玉米活性肽未超滤和分子量<1000 Da 2个组分的粒径分布、氨基酸组成、α-葡萄糖苷酶抑制活性、DPPH、ABTS^(+)自由基清除率进行比较分析。结果表...为探究胃肠消化对玉米活性肽α-葡萄糖苷酶抑制活性及抗氧化活性的影响,分别对消化前后玉米活性肽未超滤和分子量<1000 Da 2个组分的粒径分布、氨基酸组成、α-葡萄糖苷酶抑制活性、DPPH、ABTS^(+)自由基清除率进行比较分析。结果表明:玉米活性肽经体外模拟胃肠消化后,2个组分的平均粒径分别降低52.01%和66.92%,氨基酸组成变化无统计学意义,未超滤组分的α-葡萄糖苷酶抑制活性和DPPH自由基清除率分别提高至48.38%±1.88%和43.21%±2.21%,ABTS^(+)自由基清除率变化无统计学意义;分子量<1000Da组分的α-葡萄糖苷酶抑制活性和ABTS^(+)自由基清除率变化无统计学意义,DPPH自由基清除率降低了1.97%。因此,玉米活性肽能够抵抗胃肠消化,且消化产物持有更佳的降血糖及抗氧化活性,为玉米活性肽在功能食品中的应用提供了理论基础。展开更多
A small molecule peptide Leu-Asp-Tyr-Glu(LDYE) prepared from the enzymatic hydrolysate of zein was tested for its anti-oxidative activities. The effects of LDYE on the turgidity of mitochondria,the fluidity of membran...A small molecule peptide Leu-Asp-Tyr-Glu(LDYE) prepared from the enzymatic hydrolysate of zein was tested for its anti-oxidative activities. The effects of LDYE on the turgidity of mitochondria,the fluidity of membrane,the activity of cytochrome C oxidase and ATPase at subcellular level were investigated by using H\-2O\-2/Fe\+\{2+\} -induced mitochondria damage model system. It was found that the small peptide exhibited an obvious ability of removing free radicals and protecting the mitochondria from damage by oxidation. [WT5HZ]展开更多
文摘为探究胃肠消化对玉米活性肽α-葡萄糖苷酶抑制活性及抗氧化活性的影响,分别对消化前后玉米活性肽未超滤和分子量<1000 Da 2个组分的粒径分布、氨基酸组成、α-葡萄糖苷酶抑制活性、DPPH、ABTS^(+)自由基清除率进行比较分析。结果表明:玉米活性肽经体外模拟胃肠消化后,2个组分的平均粒径分别降低52.01%和66.92%,氨基酸组成变化无统计学意义,未超滤组分的α-葡萄糖苷酶抑制活性和DPPH自由基清除率分别提高至48.38%±1.88%和43.21%±2.21%,ABTS^(+)自由基清除率变化无统计学意义;分子量<1000Da组分的α-葡萄糖苷酶抑制活性和ABTS^(+)自由基清除率变化无统计学意义,DPPH自由基清除率降低了1.97%。因此,玉米活性肽能够抵抗胃肠消化,且消化产物持有更佳的降血糖及抗氧化活性,为玉米活性肽在功能食品中的应用提供了理论基础。
文摘A small molecule peptide Leu-Asp-Tyr-Glu(LDYE) prepared from the enzymatic hydrolysate of zein was tested for its anti-oxidative activities. The effects of LDYE on the turgidity of mitochondria,the fluidity of membrane,the activity of cytochrome C oxidase and ATPase at subcellular level were investigated by using H\-2O\-2/Fe\+\{2+\} -induced mitochondria damage model system. It was found that the small peptide exhibited an obvious ability of removing free radicals and protecting the mitochondria from damage by oxidation. [WT5HZ]
