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Essential oil content and composition of Indian sandalwood (Santalum album) in Sri Lanka 被引量:10
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作者 Upul Subasinghe Manuri Gamage D.S. Hettiarachchi 《Journal of Forestry Research》 SCIE CAS CSCD 2013年第1期127-130,共4页
Santalum album (Indian Sandalwood) is found in the mountainous regions of the intermediate zone of Sri Lanka. Few studies have been conducted on sandalwood ecology in this region, and ours is the first recorded stud... Santalum album (Indian Sandalwood) is found in the mountainous regions of the intermediate zone of Sri Lanka. Few studies have been conducted on sandalwood ecology in this region, and ours is the first recorded study of essential oil content and chemical composition of heartwood. We harvested two trees with State permission and took cross-sections for analysis. We demonstrated a difference in the heartwood formation and oil yield of the trees. The composition of the oil was found to be consistent between trees and along the trunk of the tree. Main aromatic compounds were santalols and other compounds are recorded in lesser quantities. Results of this study comply with the other published work on sandalwood elsewhere. This initial study on S. album in Sri Lanka provided promising results for the future of sandalwood agroforestry. 展开更多
关键词 essential oil Sri Lanka SANDALWOOD Santalum album santalol
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檀香醇对耐甲氧西林金黄色葡萄球菌USA300的抑制作用 被引量:7
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作者 谷可欣 张天翼 +3 位作者 何泾正 袁中伟 范维 尹立子 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2020年第5期594-600,共7页
采用倍比稀释法与菌落计数法,分别测定檀香醇对耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcusaureus,MRSA)标准菌株USA300的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);通过测定菌液电导率与DNA外渗量,探究檀香醇对USA30... 采用倍比稀释法与菌落计数法,分别测定檀香醇对耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcusaureus,MRSA)标准菌株USA300的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);通过测定菌液电导率与DNA外渗量,探究檀香醇对USA300细胞膜和细胞壁的影响;通过SDS–PAGE试验,探讨檀香醇对USA300可溶性蛋白代谢的作用;采用扫描电镜和透视电镜,观察经檀香醇处理后的USA300的超微结构;采用结晶紫染色法,研究檀香醇对USA300生物被膜的影响。结果表明:檀香醇能在一定程度上抑制USA300的生长繁殖,其MIC和MBC分别为32、64μg/m L;与对照组相比,经64μg/m L檀香醇处理1h后的USA300菌体电导率增加3.40%±0.54%,经64、32μg/mL檀香醇处理6 h后的菌体细胞内的DNA质量浓度显著增加(P<0.05),经64、32、16μg/m L檀香醇处理2、6 h后的菌体的可溶性蛋白均极显著降低(P<0.01);扫描电镜和透射电镜观察结果显示,檀香醇处理过的USA300菌体细胞膜和细胞壁变化不大,但是细胞的二分裂增殖出现明显的异常;亚抑菌浓度的檀香醇能明显抑制USA300生物被膜的形成。综上可知,檀香醇主要通过干扰细菌蛋白质代谢过程,可明显降低菌体内的可溶性蛋白质含量,进而影响细菌的生命活动,而对细胞膜和细胞壁的影响甚微。由此可见,檀香醇在新型抗MRSA药物的开发过程中具有较大的潜力。 展开更多
关键词 耐甲氧西林金黄色葡萄球菌 檀香醇 DNA外渗量 可溶性蛋白质 电导率 超微形态 生物被膜 抑菌作用
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